ABSTRACT
Background and Aim: Among several factors, the sperm quality of poultry is affected by the rooster's body size and the availability of antioxidants like vitamin E. This study aimed to determine the effect of dietary vitamin E supplementation on rooster sperm quality through a meta-analysis. Materials and Methods: After verification and evaluation, a total of 19 articles were included in this study. Data, including dietary vitamin E, semen volume, concentration, total sperm cells, pH, motility, viability, percentage of dead and abnormal sperm, vitamin E sperm content, malondialdehyde (MDA) content, and testosterone levels, were tabulated in a database; these were subsequently analyzed using mixed modeling with vitamin E dose as a fixed effect and study identity as a random effect. Results: Dietary supplementation level of vitamin E significantly (p<0.001) affected sperm concentration, significantly affected motility (p<0.001), significantly affected sperm vitamin E (p<0.001), significantly affected viability (p<0.001), and significantly affected chicken sperm fertility (p=0.001). Vitamin E administration also significantly reduced the number of sperm cell deaths (p<0.001); however, increased dietary levels of vitamin E did not affect semen volume (p=0.853), pH (p=0.951), MDA (p=0.542), the percentage of abnormal sperm cells (p=0.343), nor testosterone levels (p=0.063). Conclusion: Dietary vitamin E supplementation is recommended for male chickens since it generally enhances the quality of their sperm.
ABSTRACT
Background and Aim: The quality of frozen bull sperm after thawing is influenced by the primary diluent and antioxidant. This meta-analysis was conducted to determine the effect of supplementing L-cysteine and its group analogs on the quality of frozen bull sperm. Materials and Methods: A total of 22 articles obtained from Google Scholar and Scopus were integrated into metadata. The effects of adding L-cysteine and its analogs (e.g., cysteine HCl and N-acetyl-L-cysteine), both of which are known as L-cysteine, were evaluated in this meta-analysis. The following parameters were examined: Abnormality, acrosome damage, acrosomal integrity, DNA damage, DNA integrity, malondialdehyde (MDA) content, plasma membrane integrity, pregnancy rate, progressive motility, sperm viability, and total motility. Data were analyzed using the mixed model methodology, with L-cysteine dosage as a fixed effect and different studies as random effects. Results: L-cysteine supplementation significantly increased the total motility (p < 0.05) and MDA content of semen, following a linear pattern. Progressive motility, acrosomal integrity, and plasma membrane integrity were significantly increased, showing a quadratic pattern (p < 0.05). Abnormality and acrosome damage were significantly decreased (p < 0.05), following a quadratic and linear pattern, respectively. Other parameters remained unaffected by L-cysteine supplementation. L-cysteine and cysteine HCl significantly inhibited (p = 0.001) acrosome damage in thawed frozen sperm compared with control sperm. Conclusion: Supplementing L-cysteine and its analog groups are recommended for freezing bull semen as it generally improves sperm quality.
