Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.

OBJECTIVES: The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass Halophila ovalis sulfated polysaccharide on HT-29 cell line. SUBJECTS AND METHODS: Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy. The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated in vitro. RESULTS: FT-IR spectra revealed that the presence of different functional groups and the presence of galactose (82.4%), xylose (7.6%), fructose (4.0%), mannose (2.0%), fucose (1.6%), glucose (1.2%), and arabinose (1.0%) was observed using LC-MS. Ho-SP and its fractions showed radical scavenging activity in hydroxyl, 2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, and ferric reducing antioxidant power assay in a dose-dependent manner. Noticeable anti-inflammatory activity of purified fraction Ho FrIV (IC 50= 43.85 µg/ml) was observed in a noncytotoxic range of concentrations and inhibited the tumor necrosis factor-α (TNF-α)-induced interleukin-8 (IL-8) secretion (0.27 ng/ml) in HT-29 cell line. CONCLUSION: Overall, the results presented in this study suggest that purified fraction Ho FrIV of Ho-SP could suppress the TNF-α-induced secretion of IL-8 in HT-29 and thus could be used as a promising antioxidant and anti-inflammatory candidate with potential benefits.

Antinociceptive and anti-inflammatory activities of Sargassum wightii and Halophila ovalis sulfated polysaccharides in experimental animal models.

The present study investigated the effects of sulfated polysaccharides from brown seaweed Sargassum wightii (Sw-SP) and seagrass Halophila ovalis (Ho-SP) in nociceptive and inflammatory models. In the formalin test, Sw-SP and Ho-SP significantly reduced licking time in both phases of the test at a dose of 10 mg/kg. In the hot plate test, the antinociceptive effect was observed only in animals treated with 10 mg/kg of Sw-SP and 5, 10 mg/kg of Ho-SP, suggesting that the analgesic effect occurs through a central action mechanism at the higher dose. Sw-SP and Ho-SP (10 mg/kg) significantly inhibited paw edema induced by carrageenan, especially at 3 h after treatment and potentially decreased neutrophil migration by 53% and 52%, respectively. In Freund's adjuvant-induced arthritic rats, there was a significant increase in the rat paw volume and decrease in body weight, but in Sw-SP- and Ho-SP-treated groups (10 mg/kg), a significant reduction in paw volume and a normal gain in body weight were observed. The present results indicate that Sw-SP and Ho-SP possess antinociceptive and anti-inflammatory effects and have potential usefulness for development as therapeutic agents.