Screening of the antileishmanial and antiplasmodial potential of synthetic 2-arylquinoline analogs.

In this study, six analogs of 2-arylquinoline were synthesized and evaluated for their in vitro and in vivo antiplasmodial and leishmanicidal activity. At a later stage, hemolytic activity and druggability were tested in vitro and in silico, respectively, observing as a result: firstly, compounds showed half-maximal effective concentration (EC50) values between 3.6 and 19.3 µM. Likewise, a treatment using the compounds 4a-f caused improvement in most of the treated hamsters and cured some of them. Regarding the antiplasmodial activity, the compounds showed moderate to high activity, although they did not show hemolytic activity. Furthermore, 4e and 4f compounds were not able to control P. berghei infection when administered to animal models. Molecular dynamic simulations, molecular docking and ligand binding affinity indicate good characteristics of the studied compounds, which are expected to be active. And lastly, the compounds are absorbable at the hematoencephalic barrier but not in the gastrointestinal tract. In summary, ADMET properties suggest that these molecules may be used as a safe treatment against Leishmania.

Exploration of Copper Oxide Nanoneedle Electrosynthesis Applied in the Degradation of Methylene Blue.

In this study, we report a low cost, fast and unexplored electrochemical synthesis strategy of copper oxide nanoneedles films as well as their morphological and chemical characterization. The nanostructured films were prepared using electrochemical anodization in alkaline electrolyte solutions of ethylene glycol, water and fluoride ions. The film morphology shows nanoneedle-shaped structures, with lengths up to 1-2 µm; meanwhile, high-resolution X-ray photoelectron spectroscopy (HRXPS) and spectroscopy Raman analyses indicate that a mixture of Cu(II) and Cu(I) oxides, or only Cu(I) oxide, is obtained as the percentage of water in the electrolyte solution decreases. A preliminary study was also carried out for the photocatalytic degradation of the methylene blue (MB) dye under irradiation with simulated sunlight in the presence of the nanoneedles obtained, presenting a maximum degradation value of 88% of MB and, thus, demonstrating the potential characteristics of the material investigated in the degradation of organic dyes.

Structural Characterization, DFT Calculation, NCI, Scan-Rate Analysis and Antifungal Activity against Botrytis cinerea of (E)-2-{[(2-Aminopyridin-2-yl)imino]-methyl}-4,6-di-tert-butylphenol (Pyridine Schiff Base).

Botrytis cinerea is a ubiquitous necrotrophic filamentous fungal phytopathogen that lacks host specificity and can affect more than 1000 different plant species. In this work, we explored L1 [(E)-2-{[(2-aminopyridin-2-yl)imino]-methyl}-4,6-di-tert-butylphenol], a pyridine Schiff base harboring an intramolecular bond (IHB), regarding their antifungal activity against Botrytis cinerea. Moreover, we present a full characterization of the L1 by NMR and powder diffraction, as well as UV-vis, in the presence of previously untested different organic solvents. Complementary time-dependent density functional theory (TD-DFT) calculations were performed, and the noncovalent interaction (NCI) index was determined. Moreover, we obtained a scan-rate study on cyclic voltammetry of L1. Finally, we tested the antifungal activity of L1 against two strains of Botrytis cinerea (B05.10, a standard laboratory strain; and A1, a wild type strains isolated from Chilean blueberries). We found that L1 acts as an efficient antifungal agent against Botrytis cinerea at 26 °C, even better than the commercial antifungal agent fenhexamid. Although the antifungal activity was also observed at 4 °C, the effect was less pronounced. These results show the high versatility of this kind of pyridine Schiff bases in biological applications.

Green Synthesis and Electrochemical Properties of Mono- and Dimers Derived from Phenylaminoisoquinolinequinones.

In the search for new quinoid compounds endowed with potential anticancer activity, the synthesis of novel heterodimers containing the cytotoxic 7-phenylaminoisoquinolinequinone and 2-phenylaminonaphthoquinone pharmacophores, connected through methylene and ethylene spacers, is reported. The heterodimers were prepared from their respective isoquinoline and naphthoquinones and 4,4'-diaminodiphenyl alkenes. The access to the target heterodimers and their corresponding monomers was performed both through oxidative amination reactions assisted by ultrasound and CeCl3·7H2O catalysis "in water". This eco-friendly procedure was successfully extended to the one-pot synthesis of homodimers derived from the 7-phenylaminoisoquinolinequinone pharmacophore. The electrochemical properties of the monomers and dimers were determined by cyclic and square wave voltammetry. The number of electrons transferred during the oxidation process, associated to the redox potential EI1/2, was determined by controlled potential coulometry.

In-Situ Preparation of CdTe Quantum Dots Capped with a ß-Cyclodextrin-Epichlorohydrin Polymer: Polymer Influence on the Nanocrystal's Optical Properties.

ß-Cyclodextrin (ßCD), the less water soluble of the cyclodextrins, has been used as a capping agent in the preparation of semiconductor nanocrystals or quantum dots (QDs). Nevertheless, no reports have been found in the use of the highly water-soluble polymer of this, prepared by the crosslinking of the ßCD units with epichlorohydrin in basic medium (ßCDP). This polymer, besides to overcome the low solubility of the ßCD, increases the inclusion constant of the guest; two parameters that deserve its use as capping agent, instead of the native cyclodextrin. In the present manuscript, we afforded the in-situ aqueous preparation of cadmium telluride (CdTe) QDs capped with ßCDP. The polymer influence on the photoluminescent properties of the nanocrystals was analyzed. The ßCDP controls the nanocrystals growth during the Oswald ripening stage. Consequently, the CdTe capped ßCDP QDs showed lower Stokes-shift values, higher photoluminescent efficiency, and narrower size distribution than for nanocrystals obtained in the absence of polymer. Transmission electron microscopy (TEM) micrographs and energy dispersive X-ray spectroscopy (EDS) analysis revealed the composition and crystallinity of the CdTe QDs. This ßCDP capped CdTe QDs is a potential scaffold for the supramolecular modification of QDs surface.

New Properties of a Bioinspired Pyridine Benzimidazole Compound as a Novel Differential Staining Agent for Endoplasmic Reticulum and Golgi Apparatus in Fluorescence Live Cell Imaging.

In this study, we explored new properties of the bioinspired pyridine benzimidazole compound B2 (2,4-di-tert-butyl-6-(3H-imidazo[4,5-c]pyridine-2-yl)phenol) regarding its potential use as a differential biomarker. For that, we performed 1D 1HNMR (TOCSY), UV-Vis absorption spectra in different organic solvents, voltammetry profile (including a scan-rate study), and TD-DFT calculations that including NBO analyses, to provide valuable information about B2 structure and luminescence. In our study, we found that the B2 structure is highly stable, where the presence of an intramolecular hydrogen bond (IHB) seems to have a crucial role in the stability of luminescence, and its emission can be assigned as fluorescence. In fact, we found that the relatively large Stokes Shift observed for B2 (around 175 nm) may be attributed to the stability of the B2 geometry and the strength of its IHB. On the other hand, we determined that B2 is biocompatible by cytotoxicity experiments in HeLa cells, an epithelial cell line. Furthermore, in cellular assays we found that B2 could be internalized by passive diffusion in absence of artificial permeabilization at short incubation times (15 min to 30 min). Fluorescence microscopy studies confirmed that B2 accumulates in the endoplasmic reticulum (ER) and Golgi apparatus, two organelles involved in the secretory pathway. Finally, we determined that B2 exhibited no noticeable blinking or bleaching after 1 h of continuous exposure. Thus, B2 provides a biocompatible, rapid, simple, and efficient way to fluorescently label particular organelles, producing similar results to that obtained with other well-established but more complex methods.

Two New Fluorinated Phenol Derivatives Pyridine Schiff Bases: Synthesis, Spectral, Theoretical Characterization, Inclusion in Epichlorohydrin-ß-Cyclodextrin Polymer, and Antifungal Effect.

It has been reported that the structure of the Schiff bases is fundamental for their function in biomedical applications. Pyridine Schiff bases are characterized by the presence of a pyridine and a phenolic ring, connected by an azomethine group. In this case, the nitrogen present in the pyridine is responsible for antifungal effects, where the phenolic ring may be also participating in this bioactivity. In this study, we synthesized two new pyridine Schiff Bases: (E)-2-[(3-Amino-pyridin-4-ylimino)-methyl]-4,6-difluoro-phenol (F1) and (E)- 2-[(3-Amino-pyridin-4-ylimino)-methyl]-6-fluoro-phenol (F2), which only differ in the fluorine substitutions in the phenolic ring. We fully characterized both F1 and F2 by FTIR, UV-vis, 1H; 13C; 19F-NMR, DEPT, HHCOSY, TOCSY, and cyclic voltammetry, as well as by computational studies (DFT), and NBO analysis. In addition, we assessed the antifungal activity of both F1 (two fluorine substitution at positions 4 and 6 in the phenolic ring) and F2 (one fluorine substitution at position 6 in the phenolic ring) against yeasts. We found that only F1 exerted a clear antifungal activity, showing that, for these kind of Schiff bases, the phenolic ring substitutions can modulate biological properties. In addition, we included F1 and F2 into in epichlorohydrin-ß-cyclodextrin polymer (ßCD), where the Schiff bases remained inside the ßCD as determined by the ki , TGA, DSC, and SBET. We found that the inclusion in ßCD improved the solubility in aqueous media and the antifungal activity of both F1 and F2, revealing antimicrobial effects normally hidden by the presence of common solvents (e.g., DMSO) with some cellular inhibitory activity. The study of structural prerequisites for antimicrobial activity, and the inclusion in polymers to improve solubility, is important for the design of new drugs.

Peripheral CD4+CD28null T-cells as predictors of damage in systemic lupus erythematosus patients.

OBJECTIVES: To determine whether the CD4+CD28null T-cells subpopulation predicts the occurrence of damage in SLE. METHODS: This longitudinal study was conducted in consecutive SLE patients seen every six months in our Rheumatology Department since 2012. Patients in whom CD4+CD28null T-cells had been measured and who had at least one subsequent visit were included in the study. Survival analyses (univariable and multivariable Cox-regression models) were performed to determine the risk of overall and domain damage (as per the SLICC Damage Index - SDI) as a function of the frequency of this T-cell subpopulation. The multivariable model was adjusted for pertinent confounders. All analyses were performed using SPSS 21.0. RESULTS: One hundred and nineteen patients were evaluated; their mean (SD) age was 43.5 (11.9) years, 113 (95.0%) were female. Disease duration was 7.8 (7.0) years, the SLEDAI 5.3 (4.1) and the SDI 1.0 (1.4). The percentage of CD4+CD28null T-cells was 17.4 (14.0). The mean follow-up was 2.1 (0.8) years, and the mean number of visits per patient 3.5 (1.1). Forty-six (38.7%) patients increase at least one SDI point. In the univariable and multivariable analyses, the percentage of CD4+CD28null predicted the occurrence of lung damage [HR: 1.042 (CI95%: 1.001-1.085); p=0.047 and HR: 1.099 (CI95%1.020-1.184); p=0.013, respectively] but neither the total SDI score nor all other SDI domain scores were predicted by the percentage of CD4+CD28null cells. CONCLUSIONS: In SLE patients, CD4+CD28null T-cells predict the occurrence of new lung damage, independently of other risk factors but not of overall damage or damage on other domains.

Relación de hallazgos en citologíascérvicovaginales y colposcopias con infecciónpor el virus del papiloma humano, en una poblaciónde la ciudad de Santa marta / Findings in relation smears cervico vaginales and colposcopies infected with human papiloma, in a population of the city of Santa marta

Introducción:la citología cérvicovaginal es una prueba de tamizaje para detectar las neoplasias intracervicales (NIC), que ha disminuido la incidencia y la mortalidad por cáncer de cuello uterino (CCU). El diagnóstico del NIC es más frecuente entre los 20 y 30 años y el de CCU tiene una mayor frecuencia entre los 30 y 40 años. Desde 1983 a 1991 la tasa de mortalidad por CCU en Colombia fue de 6,5/100.000 habitantes y el 50% de estos casos murieron por diagnóstico tardío. Aunque se divulga que la infección por el papilomavirus es la causa necesaria, no es suficiente para el desarrollo del CCU. Objetivo:determinar una relación entre VPH y el desarrollo de NIC y CCU.Materiales y métodos:se revisaron 11.992 citologías vaginales en el período de un año, y a las VPH positivas por patología se les realizó biopsia de cérvix. Resultados:hay mayor probabilidad de adquirir VPH entre los 30 y 39 años, que se reduce en menores de 19 y mayores de 50 años. Con la sola presencia del virus la probabilidad de desarrollar NIC-I es de 5.8%, la de desarrollar NICII-III es de 0.29% y la de desarrollar CCU es de 0.08%. Conclusiones: se encontraron9 casos de CCU en 735 citologías con VPH diagnosticadas por patología, que corresponde al 1.2%; y una probabilidad del 0.08% para el desarrollo del cáncer, teniendo en cuenta solo la presencia del VPH. Este resultado sugiere que se necesitan otros factores para el desarrollo del cáncer.

Introduction: cervical Pap smear is a test to detect potentially malignant cervical neoplasia (CIN), which has decreased the incidence and mortality from cervical (cervical cancer) cancer. NIC diagnosis is most common between 20 and 30 years and the cervical cancer has a higher frequency between 30 and 40 years. From 1983 to 1991, the mortality rate for cervical cancer in Colombia was 6.5 / 100,000 and 50% of these cases died late diagnosis. Although it is accepted that infection with human papillomavirus (HPV) is the necessary cause, itis not enough for the development of cervical cancer.Objective:to determine a relationship between HPV and cervical cancer development and NIC.Materials and Methods:11,992 Pap smears were reviewed in the period of one year and a positive HPV pathologyunderwent cervical biopsy.Results:no more likely to get HPV between 30 and 39, which is reduced by less than 19 and older than 50 years. The mere presence of the virus the probability of developing CIN-I is 5.8%, to develop NICII-III is 0.29% and that of developing cervical cancer is 0.08%.Conclusions:9 cases of cervical cancer were found in 735 HPV diagnosed by cytological pathology, corresponding to 1.2%; and a probability of 0.08% to cancer development, taking into account only the presence of HPV. This result suggests that other factors for cancer development are needed.

Comparison of the catalytic activity for O2 reduction of Fe and Co MN4 adsorbed on graphite electrodes and on carbon nanotubes.

We have compared the electrocatalytic activity of several substituted and unsubstituted Co and Fe N4-macrocyclic complexes (MN4) for the electro-reduction of oxygen with the complexes directly adsorbed on the edge plane of pyrolytic graphite or adsorbed on carbon nanotubes (CNTs). In the presence of CNTs, one order of magnitude higher surface concentrations of MN4 catalysts per geometric area unit could be adsorbed leading to a lower overpotential for the oxygen electro-reduction and activities in the same order of magnitude as the commercially available Pt/C catalysts in basic pH. From Koutecky-Levich regression analysis, the total number of electrons transferred was approximately 2 for all the Co complexes and 4 for all the Fe ones, both in the presence and in the absence of the carbon nanotubes. Furthermore, the Tafel slopes did not vary due to the presence of the CNTs and presented values in the range of -0.06 V decade-1 for the CoN4 compounds and in the range of -0.04 V decade-1 for FeN4. When plotting the log of kinetic current densities (i.e. log jk) at a constant potential for each complex divided by the surface concentration Γ, and the number of electrons transferred n for the ORR for each catalyst, versus the difference between the redox potential of the metal active site of the Co(ii)/(i) or Fe(iii)/(ii) catalyst and the reversible potential of the reaction they promote, the catalytic activity increases when the formal potential of the complex becomes more positive and the data obtained with complexes adsorbed on graphite are in agreement with the data obtained when using CNTs indicating that the increase in jk when CNTs are present is only due to an increase in the number of active sites per geometric area of the electrode.

Theoretical Method for an Accurate Elucidation of Energy Transfer Pathways in Europium(III) Complexes with Dipyridophenazine (dppz) Ligand: One More Step in the Study of the Molecular Antenna Effect.

A theoretical protocol to study the sensitization and emission mechanism in lanthanide compounds on the basis of multireference CASSCF/PT2 calculations is proposed and applied to [Eu(NO3)3(dppz-CN)] and [Eu(NO3)3(dppz-NO2)] compounds synthesized and characterized herein. The method consists of a fragmentation scheme where both the ligand and the lanthanide fragments were calculated separately but at the same level of theory, using ab initio wave-function-based methods which are adequate for the treatment of quasi-degenerate states. This is based on the fact that the absorption is ligand-localized and the emission is europium-centered. This characteristic allowed us to describe the most probable energy transfer pathways that take place in the complexes, which involved an ISC between the S1 to T1 ligand states, energy transfer to 5D2 in the lanthanide fragment, and further 5D0 → 7FJ emission. For both compounds, the triplet and 5D2 states were determined at the CASPT2 level to be around ∼26000 and ∼22400 cm-1, respectively. This difference is in the optimal range for the energy transfer process. Finally, the emissive state 5D0 was found at ∼18000 cm-1 and the emission bands in the range 550-700 nm, in quite good agreement with the experimental results.

Relación de la enfermedad periodontal con el parto pretérmino en gestantes en Arequipa / Relationship of periodontal disease with preterm delivery in pregnant women in Arequipa

Introducción: Esta investigación tuvo como propósito investigar la relación entre la enfermedad periodontal y el parto prematuro, debido a la diferencia de opiniones que existe en cuanto a la participación de la enfermedad periodontal en el desarrollo de enfermedades perinatales adversas. Métodos: Se realizó el examen periodontal mediante el Índice de Necesidad de Tratamiento Periodontal de la Comunidad, examinando 49 gestantes que acudieron a labor de parto al Hospital Nacional Carlos Albero Seguín Escobedo entre los meses de julio y agosto del 2014, los datos de tiempo gestacional fue recabo mediante la revisión de las historias clínicas. Resultados: Se encontró relación inversa entre el tiempo de gestación y el índice enfermedad periodontal; las gestantes que solo presentaron sangrado gingival tuvieron un tiempo gestacional de 38.8 semanas, mientras que en quienes existió presencia de placa y sangrado la media fue de 36.8 semanas de gestación, para las gestantes con presencia de bolsas entre 4 y 5 mm la media fue de 34.6 semana.sConclusiones: Se determinó que si existe relación entre la enfermedad periodontal y el parto prematuro en las gestantes resultando que 32 mujeres estudiadas (65.3%) presentaron enfermedad periodontal entre moderada y severa, adicionalmente 17 gestantes no presentaron parto prematuro, representando el 34.7% de la muestra evaluada, las cuales presentaron enfermedad periodontal leve. (AU)

Introduction: This research was aimed to investigate the relationship between periodontal disease and preterm delivery, due to the difference of opinion exists as to the involvement of periodontal diseasein the development of adverse perinatal diseases. Methods: Was the periodontal examination using the index of need of community Periodontal treatment, examining 49 women who attended the Hospital National Carlos Albero Seguin Escobedo between the months of July and August of 2014 to of labor, gestational time data was collected through the review of the medical records. Results: Was found inverse relationship between gestational age and the periodontal disease index; the pregnant who only presented gingival bleeding had a gestational period of 38.8 weeks, while in those who existed presence of plaque and bleeding had a mean of 36.8 weeks of gestation, pregnant with presence of bags between 4 mm and 5 mm for the mean was 34.6 weeks Conclusions: It was determined that if there is a relationship between periodontal disease and preterm birth in pregnant resulting to 32 women studied (65.3%) presented moderate to severe periodontal disease, in addition 17 pregnant women did not show premature birth, representing 34.7% of the evaluated sample, which showed mild periodontal disease. (AU)

Circulating naive and memory CD4+ T cells and metabolic syndrome in patients with systemic lupus erythematosus: data from a primarily Mestizo population.

OBJECTIVE: The aim of this study was to determine whether the proportions of naive and memory CD4(+) T cell are independently associated with the metabolic syndrome (MetS) in patients with SLE. METHODS: This cross-sectional study was conducted in SLE patients seen at our rheumatology department between September 2013 and April 2014. CD4(+) T cell subpopulations were examined by flow cytometry. The association of MetS and CD4(+) T cell subpopulations was examined by Mann-Whitney U-test and by multivariable analysis, adjusting for all possible confounding variables. RESULTS: One hundred and seventeen patients were evaluated. Their mean age was 44.6 years (S.D. 12.6), 109 (93.2%) were female and all patients were Mestizo (mixed Caucasian and Amerindian ancestry). Fifty-two patients (44.4%) presented with MetS. Disease duration was 7.6 years (S.D. 6.8). The percentage of naive CD4(+) T cells was 25.0 (S.D. 12.7) and memory CD4(+) T cells was 66.7 (S.D. 13.2) and the memory:naive CD4(+) T cell ratio was 4.3 (S.D. 5.6). In multivariable analysis, the percentage of naive CD4(+) T cells was negatively associated with the presence of MetS [odds ratio (OR) 0.959 (95% CI 0.923, 0.997), P = 0.033], whereas the percentage of memory CD4(+)T cells and the memory:naive CD4(+) T cell ratio were positively associated with its presence [OR 1.040 (95% CI 1.003, 1.078), P = 0.031 and OR 1.238 (95% CI 1.041, 1.472), P = 0.016, respectively]. CONCLUSION: In the SLE patients studied, a lower percentage of naive CD4(+) T cells, a higher percentage of memory CD4(+) T cells and the memory:naive CD4(+) T cell ratio were independently associated with the presence of MetS. This association could reflect the impact of immunosenescence among SLE patients with cardiovascular morbidity.

[Complications of conventional and laparoscopic gastric bypass. Prospective and comparative study]. / Complicaciones del bypass gástrico convencional y laparoscópico. Estudio prospectivo y comparativo.

BACKGROUND: Roux-en-y gastric bypass for the management of morbid obesity is currently the safest and more efficient treatment option. the laparoscopic era has shown its benefits in this type of surgery, so some authors advocate its implementation in a practical systematic way. all of which has lead in recent years to the unavoidable change in the surgical approach. OBJECTIVES: To evaluate and compare the complications of laparoscopic gastric bypass and the conventional one in patients with morbid obesity. Application site: self-administration national university hospital. DESIGN: Comparative, prospective no randomized study. POPULATION: Between 2002 and 2011, 420 morbidly obese patients received surgical treatment for the obesity either open (167) or laparoscopic (253) surgery RESULTS: Early postoperative complication rate was 4% in the lbg and 34% in the cbg (p < 05, 95%ci 22-38), mostly represented by seromas (25%) and wall abscesses (5.4%). the percentage of late postoperative complications was 2.8% in the lbg group and 9.6% in the cbg (p=0.004, 95%ci 1.4-12.2), the most frequent being incisional hernias (7.18%). CONCLUSIONS: According to our experience and consulted literature, laparoscopic gastric bypass is a safe, reproducible and with a lower rate of both early and late complications than cbg, and so we consider and offer lbg as the ideal technique for the treatment of patients with morbid obesity.

Antecedentes: el bypass gástrico en y de roux para el tratamiento de la obesidad mórbida representa hoy en día la opción terapéutica más segura y eficiente. la era laparoscópica ha demostrado sus beneficios en este tipo de cirugía, por lo que algunos autores abogan por su implementación en forma casi sistemática, conduciendo en los últimos años al inexorable cambio en la vía de abordaje. Objetivos: evaluar y comparar las complicaciones del bypass gástrico laparoscópico y convencional en pacientes con obesidad mórbida. Lugar de aplicación: hospital nacional universitario de autogestión. Diseño: estudio comparativo, prospectivo no randomizado. Población: entre el 2002 y 2011, 420 pacientes con obesidad mórbida recibieron un tratamiento quirúrgico para la obesidad ya sea en forma laparoscópica (253) o convencional (167). Resultados: el porcentaje de complicaciones postoperatorias tempranas fue del 4% en el grupo bgl y del 34% en el bgc (p <05, ic95% 22 - 38), representadas en su mayoría por seromas (25%) y abscesos de pared (5,4%). el porcentaje de complicaciones postoperatorias tardías fue del 2,8% en el grupo bgl y del 9,6% en el bgc (p= 0.004, ic95% 1,4 - 12,2), siendo las más frecuentes las eventraciones (7,2%). Conclusiones: de acuerdo a nuestra experiencia y a la literatura consultada, el bypass gástrico laparoscópico es un procedimiento seguro, reproducible y con una menor tasa de complicaciones tanto tempranas como tardías que el bg c, por lo que consideramos y ofrecemos al bgl como técnica ideal en el tratamiento de los pacientes con obesidad mórbida.

Complicaciones del bypass gástrico convencional y laparoscópico. Estudio prospectivo y comparativo / [Complications of conventional and laparoscopic gastric bypass. Prospective and comparative study].

BACKGROUND: Roux-en-y gastric bypass for the management of morbid obesity is currently the safest and more efficient treatment option. the laparoscopic era has shown its benefits in this type of surgery, so some authors advocate its implementation in a practical systematic way. all of which has lead in recent years to the unavoidable change in the surgical approach. OBJECTIVES: To evaluate and compare the complications of laparoscopic gastric bypass and the conventional one in patients with morbid obesity. Application site: self-administration national university hospital. DESIGN: Comparative, prospective no randomized study. POPULATION: Between 2002 and 2011, 420 morbidly obese patients received surgical treatment for the obesity either open (167) or laparoscopic (253) surgery RESULTS: Early postoperative complication rate was 4

in the lbg and 34

in the cbg (p < 05, 95

ci 22-38), mostly represented by seromas (25

) and wall abscesses (5.4

). the percentage of late postoperative complications was 2.8

in the lbg group and 9.6

in the cbg (p=0.004, 95

ci 1.4-12.2), the most frequent being incisional hernias (7.18

). CONCLUSIONS: According to our experience and consulted literature, laparoscopic gastric bypass is a safe, reproducible and with a lower rate of both early and late complications than cbg, and so we consider and offer lbg as the ideal technique for the treatment of patients with morbid obesity.

Complicaciones del bypass gástrico convencional y laparoscópico. Estudio prospectivo y comparativo. / [Complications of conventional and laparoscopic gastric bypass. Prospective and comparative study].

BACKGROUND: Roux-en-y gastric bypass for the management of morbid obesity is currently the safest and more efficient treatment option. the laparoscopic era has shown its benefits in this type of surgery, so some authors advocate its implementation in a practical systematic way. all of which has lead in recent years to the unavoidable change in the surgical approach. OBJECTIVES: To evaluate and compare the complications of laparoscopic gastric bypass and the conventional one in patients with morbid obesity. Application site: self-administration national university hospital. DESIGN: Comparative, prospective no randomized study. POPULATION: Between 2002 and 2011, 420 morbidly obese patients received surgical treatment for the obesity either open (167) or laparoscopic (253) surgery RESULTS: Early postoperative complication rate was 4

in the lbg and 34

in the cbg (p < 05, 95

ci 22-38), mostly represented by seromas (25

) and wall abscesses (5.4

). the percentage of late postoperative complications was 2.8

in the lbg group and 9.6

in the cbg (p=0.004, 95

ci 1.4-12.2), the most frequent being incisional hernias (7.18

). CONCLUSIONS: According to our experience and consulted literature, laparoscopic gastric bypass is a safe, reproducible and with a lower rate of both early and late complications than cbg, and so we consider and offer lbg as the ideal technique for the treatment of patients with morbid obesity.

[Right iliac fossa pain and Alvarado Score]. / Dolor en fosa ilíaca derecha y Score de Alvarado.

INTRODUCTION: Unsuspected or undiagnosed acute appendicitis could progress to a perforation or could lead to the removal of a normal appendix. OBJECTIVES: using a clinical score system as a diagnostic tool for this pathology and can decrease negative appendectomies. MATERIAL AND METHOD: Prospective and protocolized study. SETTING: National Clinicas Hospital. Córdoba. Argentina. Between May 2007 and June 2007 224 patients were studied of which 117 were male and 107 were female with a mean age of 26.65 years. An Alvarado score was performed on all patients on admission. Based on the clinical evidence and a second assessment with the Alvarado Score, surgical exploration was decided in 207 patients. The remaining 17 were excluded due to other pathology. The diagnosis of acute appendicitis was confirmed by surgical finding and histopathological studies. RESULTS: In the surgical findings it was shown that 189 (91%) patients had acute appendicitis. There was no surgical mortality. As regards morbidity, 3.86% had medical and 27% had surgical complications. The histopathology reports showed a normal caecal appendix in 18. Whereas the incidence of negative appendicectomy was about 8.69%. CONCLUSIONS: The use of the Alvarado Score together with the surgical and histopathology findings confirmed it was sensitive from 6 points for the diagnosis of acute appendicitis.

Dolor en fosa ilíaca derecha y Score de Alvarado / Right iliac fossa pain and Alvarado Score

Introducción. La apendicitis aguda no sospechada ni diagnosticada puede evolucionar hacia la perforación o, por el contrario, conduce a la remoción de un apéndice normal. El objetivo del estudio fue utilizar un sistema de clasificación, desde un punto de vista clínico, para el diagnóstico de esta enfermedad y lograr disminuir las apendicectomías negativas. Material y método. Estudio prospectivo y protocolizado, que se aplicó en el Hospital Nacional de Clínicas, Córdoba (Argentina). Comprende a 224 pacientes estudiados entre mayo de 2004 y junio de 2007. De ellos, 117 eran varones y 107, mujeres, con una edad promedio de 26,65 años. A todos los pacientes se les realizó al ingreso el Score de Alvarado. Según la clínica y una segunda valoración con el Score se decidió la exploración quirúrgica en 207 pacientes. Se excluyó a los 17 restantes por otras enfermedades. El diagnóstico de apendicitis aguda fue confirmado por los hallazgos quirúrgicos y la anatomía patológica. Resultados. En los hallazgos operatorios hubo 189 (91,3%) pacientes que tenían apendicitis aguda. No hubo mortalidad postoperatoria. En relación con la morbilidad hubo un 3,86% de complicaciones médicas y un 27% quirúrgicas. El informe anatomopatológico mostró en 18 pacientes un apéndice cecal normal. Por lo tanto, la incidencia de las apendicectomías negativas fue del 8,69%. Conclusiones. La utilización del Score en relación con los hallazgos quirúrgicos y anatomopatológicos confirma que fue sensible a partir de 6 puntos para el diagnóstico de apendicitis aguda (AU)

Introduction. Unsuspected or undiagnosed acute appendicitis could progress to a perforation or could lead to the removal of a normal appendix. Objectives: using a clinical score system as a diagnostic tool for this pathology and can decrease negative appendectomies. Material and method. Prospective and protocolized study. Setting: National Clinicas Hospital. Córdoba. Argentina. Between May 2007 and June 2007 224 patients were studied of which 117 were male and 107 were female with a mean age of 26.65 years. An Alvarado score was performed on all patients on admission. Based on the clinical evidence and a second assessment with the Alvarado Score, surgical exploration was decided in 207 patients. The remaining 17 were excluded due to other pathology. The diagnosis of acute appendicitis was confirmed by surgical finding and histopathological studies. Results. In the surgical findings it was shown that 189 (91%) patients had acute appendicitis. There was no surgical mortality. As regards morbidity, 3.86% had medical and 27% had surgical complications. The histopathology reports showed a normal caecal appendix in 18. Whereas the incidence of negative appendicectomy was about 8.69%. Conclusions. The use of the Alvarado Score together with the surgical and histopathology findings confirmed it was senstive from 6 points for the diagnosis of acute appendicitis (AU)

Methoxycarbonylation of olefins catalyzed by palladium complexes bearing P,N-donor ligands.

The methoxycarbonylation of alkenes catalyzed by palladium(II) complexes with P,N-donor ligands, 2-(diphenylphosphinoamino)pyridine (Ph2PNHpy), 2-[(diphenylphosphino)methyl]pyridine (Ph2PCH2py), and 2-(diphenylphosphino)quinoline (Ph2Pqn) has been investigated. The results show that the complex [PdCl(PPh3)(Ph2PNHpy)]Cl or an equimolar mixture of [PdCl2(Ph2PNHpy)] and PPh3, in the presence of p-toluensulfonic acid (TsOH), is an efficient catalyst for this reaction. This catalytic system promotes the conversion of styrene into methyl 2-phenylpropanoate and methyl 3-phenylpropanoate with nearly complete chemoselectivity, 98% regioselectivity in the branched isomer, and high turnover frequency, even at alkene/Pd molar ratios of 1000. Best results were obtained in toluene-MeOH (3 : 1) solvent. The Pd/Ph2PNHpy catalyst is also efficient in the methoxycarbonylation of cyclohexene and 1-hexene, although with lower rates than with styrene. Related palladium complexes [PdCl(PPh3)L]Cl (L = Ph2PCH2py and Ph2Pqn) show lower activity in the methoxycarbonylation of styrene than that of the 2-(diphenylphosphinoamino)pyridine ligand. Replacement of the last ligand by (diphenylphosphino)phenylamine (Ph2PNHPh) or 2-(diphenylphosphinoaminomethyl)pyridine (Ph2PNMepy) also reduces significantly the activity of the catalyst, indicating that both the presence of the pyridine fragment as well as the NH group, are required to achieve a high performing catalyst. Isotopic labeling experiments using MeOD are consistent with a hydride mechanism for the [PdCl(PPh3)(Ph2PNHpy)]Cl catalyst.