Cost benefit analysis of diversified farming systems across Europe: Incorporating non-market benefits of ecosystem services.

Crop diversification can enhance farm economic sustainability while reducing the negative impact on the environment and ecosystem services related. Despite the market and non-market benefits of crop diversification, monocropping is a widely used dominant practice in Europe. In this context, this works aims to assess the overall economic impact of several crop diversification systems across Europe and compared it to the monocropping system. For this purpose, an economic valuation by integrating market and non-market values for eight case studies distributed across three different European pedoclimatic regions (Southern Mediterranean, Northern Mediterranean and Boreal) is proposed. The economic valuation was conducted both in the short and medium-long term. For the short-term we conducted a social gross margin analysis, while for the medium-long term a cost-benefit analysis is developed. The results show an improvement in social gross margins for most of the diversification scenarios assessed when environmental and socio-cultural benefits are considered in the short-term. In the medium and long-term the transformation of cropping towards a more diversified agriculture is encouraged by greater economic benefits. These results provide a first insight in global economic performance of diversified cropping systems, whose main contribution relies on the integration of market and non-market values of ecosystem services from crop diversification. They are expected to be useful for guiding policy makers to promote crop diversification practices as a key instrument for building resilience in farming systems for an adaptive management to climate change.

Are citizens willing to pay for the ecosystem services supported by Common Agricultural Policy? A non-market valuation by choice experiment.

Public attention to the impact of human activities on the environment is growing over time across several sectors, particularly agri-food. In Europe, the agricultural sector's focus on sustainability has influenced EU policies for at least 40 years. The Common Agricultural Policy (CAP) has for years been proposing tools, commitments, and incentives to mitigate the over-use of natural resources and to increase or maintain the flow of ecosystem services (ES) provide by agro-ecosystems. In the latest reform (23-27), the EU strengthens the commitments required of farmers for environmental issues. The role of farmers in natural capital management and ES provision seems to be recognized, EU subsidies for farmers seem to be more related to sustainability and well-being concerns of European citizen. However, it is necessary to understand whether society recognizes these benefits and legitimizes this transfer of public money for these purposes. This study aims to evaluate non-farmers citizen preference through a Choice Experiment for the potential higher flows of ES provided by three of the reformulated and new Good Agricultural Environmental Conditions - GAEC. The case study developed in Italy collected data from 185 citizens of the Po Valley, one of the most intensely cultivated areas in Europe. Analyses demonstrated how society recognizes the benefits provided by more sustainable agricultural systems, showing a preference for higher ES flows. The results show that there is a hypothetical value recognized by society for ES attributable to new GAECs that will be implemented by CAP farmers. In the case study, this value is higher than what farmers currently receive for general environmental purposes through direct payment for the management of arable land. Analysis it could justify that efforts required by the new CAP reform (23-27) to the farmers to achieve sustainable agricultural systems could be compensate and supported by positive citizens valuation.

A comprehensive approach for agroecosystem services and disservices valuation.

The use of the ecosystem services approach for ecosystem management, including the valuation of ecosystem services, has grown in recent decades. Although a common framework is used, each ecosystem has its own characteristics. The agroecosystem, for example, is an anthropised ecosystem where ecosystem service flows are highly interrelated with the environment, positively or negatively. Therefore, agroecosystem services are usually accompanied by disservices. The valuation of agroecosystem services and disservices requires adaptation of existing ecosystem services paradigms to accommodate the innate agroecosystem idiosyncrasies. To this end, in this study, a comprehensive approach for valuation of agroecosystem services and disservices was proposed and validated in a semi-arid western Mediterranean agricultural area through stakeholder assessment, using a choice experiment. The results suggest that all categories of services (provisioning, regulating, and cultural) should be taken into account when valuing agroecosystem services and disservices. In particular, food provision (a provisioning service), water (a provisioning disservice), local climate regulation and biodiversity (regulating services), waste treatment and water purification (regulating disservices), and recreation and tourism (cultural services) are relevant for this purpose. Their relative importance in agroecosystems valuation reached 70% for agroecosystem services and 30% for disservices. Specifically, biodiversity (38%) emerged as the most relevant agroecosystem service to be valued, followed by recreation and tourism (20%), local climate regulation (7%), and food provision (5%). Among the agroecosystem disservices, water and waste treatment (15%), and water purification (15%) together contributed to 30% of the total importance. Agroecosystems should be valued considering their multifunctional character and the integration of agroecosystem services and disservices.

Association between Mycobacterium tuberculosis lineage and site of disease in Florida, 2009-2015.

BACKGROUND: Mycobacterium tuberculosis is characterized into four global lineages with strong geographical restriction. To date one study in the United States has investigated M. tuberculosis lineage association with tuberculosis (TB) disease presentation (extra-pulmonary versus pulmonary). We update this analysis using recent (2009-2015) data from the State of Florida to measure lineage association with pulmonary TB, the infectious form of the disease. METHODS: M. tuberculosis lineage was assigned based on the spacer oligonucleotide typing (spoligotyping) patterns. TB disease site was defined as exclusively pulmonary or extra-pulmonary. We used ORs to measure the association between M. tuberculosis lineages and pulmonary compared to extra-pulmonary TB. The final multivariable model was adjusted for patient socio-demographics, HIV and diabetes status. RESULTS: We analyzed 3061 cases, 83.4% were infected with a Euro-American lineage, 8.4% Indo-Oceanic and 8.2% East-Asian lineage. The majority of the cases (86.0%) were exclusively pulmonary. Compared to the Indo-Oceanic lineage, infection with a Euro-American (AOR=1.87, 95% CI: 1.21, 2.91) or an East-Asian (AOR=2.11, 95% CI: 1.27, 3.50) lineage favored pulmonary disease compared to extra-pulmonary. In a sub-analysis among pulmonary cases, strain lineage was not associated with sputum smear positive status, indicating that the observed association with pulmonary disease is independent of host contagiousness. CONCLUSION: As an obligate pathogen, M. tuberculosis' fitness is directly correlated to its transmission potential. In this analysis, we show that M. tuberculosis lineage is associated with pulmonary disease presentation. This association may explain the predominance in a region of certain lineages compared to others.

Spatiotemporal Clustering of Mycobacterium tuberculosis Complex Genotypes in Florida: Genetic Diversity Segregated by Country of Birth.

BACKGROUND: Tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC). Although the MTBC is highly clonal, between-strain genetic diversity has been observed. In low TB incidence settings, immigration may facilitate the importation of MTBC strains with a potential to complicate TB control efforts. METHODS: We investigated the genetic diversity and spatiotemporal clustering of 2,510 MTBC strains isolated in Florida, United States, between 2009 and 2013 and genotyped using spoligotyping and 24-locus MIRU-VNTR. We mapped the genetic diversity to the centroid of patient residential zip codes using a geographic information system (GIS). We assessed transmission dynamics and the influence of immigration on genotype clustering using space-time permutation models adjusted for foreign-born population density and county-level HIV risk and multinomial models stratified by country of birth and timing of immigration in SaTScan. PRINCIPAL FINDINGS: Among the 2,510 strains, 1,245 were reported among foreign-born persons; including 408 recent immigrants (<5 years). Strain allelic diversity (h) ranged from low to medium in most locations and was most diverse in urban centers where foreign-born population density was also high. Overall, 21.5% of cases among U.S.-born persons and 4.6% among foreign-born persons clustered genotypically and spatiotemporally and involved strains of the Haarlem family. One Haarlem space-time cluster identified in the mostly rural northern region of Florida included US/Canada-born individuals incarcerated at the time of diagnosis; two clusters in the mostly urban southern region of Florida were composed predominantly of foreign-born persons. Both groups had HIV prevalence above twenty percent. CONCLUSIONS/SIGNIFICANCE: Almost five percent of TB cases reported in Florida during 2009-2013 were potentially due to recent transmission. Improvements to TB screening practices among the prison population and recent immigrants are likely to impact TB control. Due to the monomorphic nature of available markers, whole genome sequencing is needed to conclusively delineate recent transmission events between U.S. and foreign-born persons.

Maintenance Therapy with 3-monthly Bacillus Calmette-Guérin for 3 Years is Not Superior to Standard Induction Therapy in High-risk Non-muscle-invasive Urothelial Bladder Carcinoma: Final Results of Randomised CUETO Study 98013.

BACKGROUND: Bacillus Calmette-Guérin (BCG) maintenance therapy for 3 yr following BCG induction can reduce the progression of urothelial bladder carcinoma versus BCG induction alone, but is associated with high toxicity. OBJECTIVE: To investigate whether a modified 3-yr BCG maintenance regimen following induction therapy is more effective than standard BCG induction therapy alone and exhibits a low toxicity profile. DESIGN, SETTING, AND PARTICIPANTS: Patients from the outpatient clinics of the participating centres with high-risk non-muscle-invasive bladder carcinoma (NMIBC) were randomised between October 1999 and April 2007. INTERVENTION: Participants received BCG induction once-weekly for 6 wk (no maintenance arm) or BCG induction followed by one BCG instillation every 3 mo for 3 yr (maintenance arm). OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Primary endpoints were disease-free interval (DFI) and time to progression (TTP). Secondary endpoints included survival duration and toxicity. Differences between treatment arms were tested using Student's t test and χ(2) and log-rank tests. RESULTS AND LIMITATIONS: A total of 397 patients were randomised, 195 to the no-maintenance and 202 to the maintenance arm. A median time to recurrence was not reached in either treatment arm. DFI was similar between the arms (hazard ration [HR] 0.83; 95% CI 0.61-1.13; p=0.2) with disease relapse at 5 yr of 33.5% and 38.5%, respectively. TTP was also similar between the treatment arms (HR 0.79; 95% CI 0.50-1.26; p=0.3), with a progression rate at 5 yr of 16% and 19.5%, respectively. There were no significant differences between the treatment groups for overall survival and cancer-specific survival at 5 yr. Twenty and five patients in the maintenance and no-maintenance arms, respectively, stopped treatment because of toxicity. The most common local side effects were frequency (65% of patients), dysuria (63%), and haematuria (43%); the most frequent systemic side effects were general malaise (7.2%) and fever (34%). CONCLUSIONS: In NMIBC patients treated with maintenance therapy comprising a single BCG instillation every 3 mo for 3 yr following standard induction BCG, we did not observe a decrease in recurrence and progression rates versus induction therapy alone. PATIENT SUMMARY: Patients who undergo surgery to remove bladder cancer are usually treated with bacillus Calmette-Guérin (BCG) for 6 wk if there is a high risk of disease recurrence. Extending BCG therapy by 3 yr can further minimise disease recurrence and progression, but is associated with more side effects. We report that a modified 3-yr BCG treatment regimen showed low toxicity, but seemed to be no more effective than 6-wk treatment. TRIAL REGISTRATION: CUETO 98013.

Determination of normal human intrathyroidal iodine in Caracas population.

This study focuses on the determination of iodine content in healthy thyroid samples on male population from Caracas, Venezuela. Contribution to establish a baseline of iodine content in thyroid glands and hence to compare the iodine thyroid concentration of the Venezuelan population with other countries is also our objective. Male post-mortem individual samples were analyzed using a spectrophotometric flow injection method, based on the Sandell-Kolthoff reaction. The median intrathyroidal iodine concentration was 1443+/-677 microg/g (wet weight), ranging from 419 to 3430 microg/g, which corresponds to a median of total iodine content of 15+/-8 mg (ranging from 4 to 37). These results were higher than those values reported in the literature. No correlation of iodine content with age or weight of the healthy gland was found.

Pseudoaneurisma tras autotrasplante renal. Corrección endovascular / Pseudoaneurysm following kidney autotransplantation. Endovascular correction

Introducción. Los pseudoaneurismas son una rara complicación después de un trasplante renal y prácticamente son excepcionales tras cirugía de banco y autotrasplante. En presencia de dichas lesiones se considera obligatoria su corrección con objeto de evitar el riesgo potencial de complicaciones mortales asociadas a la ruptura diferida del pseudoaneurisma. La reparación convencional es un procedimiento complejo que puede verse dificultado por los problemas inherentes asociados a una redirección. Caso clínico. Mujer de 52 años con un pseudoaneurisma de arteria ilíaca desarrollado tras cirugía ex vivo y autotrasplante renal por aneurisma de arteria renal. Las pruebas de imagen pusieron de manifiesto la lesión arterial en la ubicación de un bypass ilíacorrenal concomitante a la cirugía previa. La corrección endovascular del pseudoaneurisma se llevó a cabo mediante la liberación de un stent cubierto. La arteriografía de control mostró la completa exclusión del pseudoaneurisma de arteria ilíaca y la preservación completa del flujo en la arteria renal reconstruida. Conclusión. La exclusión del pseudoaneurisma con preservación completa del flujo del riñón trasplantado pone de relieve la potencial utilidad del tratamiento endovascular en pacientes seleccionados

Introduction. Pseudoaneurysms are a rare complication after a kidney transplant and are practically unknown following bench surgery and autotransplantation. Such lesions must be corrected in order to avoid the potential risk of fatal complications that are associated with the delayed rupture of a pseudoaneurysm. Conventional repair is a complex procedure that can be further complicated by the problems inherent to any intervention involving redirection. Case report. A 52-year-old female with a pseudoaneurysm in the iliac artery that developed after ex vivo surgery and kidney autotransplantation due to an aneurysm in the renal artery. Imaging tests showed the arterial lesion to be situated at the site of an iliac-renal bypass that had been carried out during the previous intervention. Endovascular correction of the pseudoaneurysm was performed by deploying a covered stent. The control arteriography showed the complete exclusion of the pseudoaneurysm in the iliac artery and full preservation of blood flow in the reconstructed renal artery. Conclusion. The exclusion of the pseudoaneurysm with full preservation of flow in the transplanted kidney highlights the potential value of endovascular treatment in selected patients