Interobserver Variability in Semen Analysis: Findings From a Quality Control Initiative.

Introduction For laboratory tests, precision and accuracy are indispensable to ensure reliable results for both clinical diagnosis and research endeavors. The accuracy and reliability of results are important because they have an impact on both patient management and research. In this study, we evaluated the inter-observer variability between a trained technician and two academic residents, which acted both as a quality control measure as well as an assessment of training outcomes. Material and methods Freshly obtained semen samples from 28 subjects coming to the andrology laboratory were used. Semen analysis was performed by a regular technician permanently posted in the laboratory and two residents on completion of their posting in the andrology laboratory. All three examined the same sample after liquefaction for assessment of sperm motility, sperm concentration, sperm vitality, and sperm morphology. Semen analysis was done as per the recommendations of the WHO. Results The results of the study are presented as a coefficient of variation (CV), S charts, and Bland-Altman plot where we evaluated the interobserver variability in parameters on semen analysis of the same sample by three different assessors. The mean CV for sperm concentration across the samples was 6.24%. For sperm vitality, sperm morphology, and sperm motility the mean CV was 10.14%, 2.66%, and 8.11%, respectively. The S chart and Bland-Altman plot found a few random errors in measurements. Conclusion Regular quality control assessments are essential and should be implemented in andrology laboratories to ensure accurate and reliable results. Proper training of laboratory personnel is also vital for consistent outcomes. Other measures such as equipment calibration, use of high-quality reagents, and standard reporting are also crucial for the best results from a laboratory.

Role of Hematological Parameters in the Grading of COVID-19 and a Model to Predict the Outcome in Inpatients.

Introduction Human coronaviruses, identified in the 1960s, are known culprits of respiratory infections. Classified into alpha, beta, gamma, and delta subgroups, these viruses have the capacity to transition from animal reservoirs to causing severe respiratory ailments in humans. Notable outbreaks like the 2003 severe acute respiratory distress syndrome (SARS) epidemic and the ongoing coronavirus disease 2019 (COVID-19) pandemic underscore the recurring emergence of novel coronaviruses with severe human infection potential. COVID-19, driven by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly become a leading global cause of severe acute respiratory syndrome. Immune system disruptions and cytokine imbalances contribute to severe cases, necessitating early diagnosis and precise severity assessment. Methodology This retrospective cross-sectional study encompassed 211 COVID-19 patients admitted to AIIMS Patna from May to July 2020. Clinical and hematological parameters, including neutrophils, eosinophils, basophils, lymphocytes, monocytes, red and white blood cell counts, platelet count, C-reactive protein (CRP), serum ferritin, and d-dimer, were meticulously recorded. Patients were categorized into non-severe and severe groups using the National Early Warning Score (NEWS) 2. Results  Our findings underscore the pivotal role of hematological markers in gauging COVID-19 severity. Notably, markers such as neutrophil-to-lymphocyte ratio (NLR), derived NLR, lymphocyte monocyte ratio, platelet lymphocyte ratio, d-dimer, CRP, and serum ferritin exhibited notable elevation in severe cases. Survival analysis further established the predictive potential of these markers in assessing disease progression and mortality risk. We advocate for the integration of these markers into existing severity assessment frameworks to foster objective clinical evaluations. Conclusion In conclusion, our study unravels the intricate connection between COVID-19 severity and hematological parameters. We emphasize the early warning capabilities of NLR, derived NLR, platelet lymphocyte ratio, and other markers in predicting disease progression. This research underscores the imperative need to incorporate hematological markers into the evaluation of COVID-19 severity, thereby providing invaluable insights for enhancing clinical practice and patient outcomes.

The Effect of Age on Semen Quality Among Male Partners of Infertile Couples: An Observational Study in a Tertiary Care Center in Eastern India.

INTRODUCTION: Male infertility contributes to a significant proportion of infertility cases, and advanced paternal age has been suggested to affect semen quality and fertility. However, the relationship between age and semen quality remains inconclusive, with conflicting findings reported in the literature. This study aimed to investigate the effect of age on semen quality among male partners of infertile couples in a tertiary care center in eastern India. METHODS: A cross-sectional observational study was conducted, involving 390 male participants aged 21-50 years, who were referred to the andrology laboratory for semen analysis between January 2019 and December 2022. Participants were categorized into three age groups (21-30, 31-40, and 41-50 years). Semen parameters, including sperm concentration, semen volume, motility, and morphology, were assessed according to the World Health Organization guidelines. RESULTS: Among the participants, no significant differences were observed in semen volume, motility, and morphology across different age groups. However, a statistically significant difference in sperm concentration among the three age groups was observed (p = 0.022). Spearman correlation analysis revealed a positive correlation between age and sperm concentration (r = 0.124, p = 0.013) as well as total sperm count (r = 0.10, p = 0.049). CONCLUSION: In this study, no significant decline in semen quality with age was found among male partners of infertile couples aged 21-50 years. These findings highlight the complex relationship between age and semen quality and emphasize the need for further research to better understand the underlying mechanisms and provide more conclusive evidence regarding the impact of age on male fertility.

The Electroencephalographic Characterization of Hypsarrhythmia in Older Pediatric Population With Epilepsy Using Computer-Added Quantitative Methods.

Background Hypsarrhythmia is a classical multifocal electroencephalographic finding in patients of infantile spasm and related epileptic syndromes of early childhood including West syndrome and Otahara syndrome. It usually presents in early infancy and persists up to the age of two years, after which it usually resolves. The persistence of hypsarrhythmia beyond the age of two years has rarely been reported in the literature. The present study is an attempt to investigate and compare the origin and activation pattern of epileptic activity between the subjects aged 3-10 years with and without hypsarrythmia. Material and methods Forty-one patients in the age group of 3-10 years with features suggestive of seizure have been studied for quantitative electroencephalographic characteristics after dividing into hypsarrythmic and normal seizure patterns. Result The power spectral density (PSD) of 15 patients with hypsarrhythmia showed a significantly predominant delta frequency in quantitative electrography (qEEG) in comparison to the seizure subjects with normal electroencephalography (EEG) patterns. The amplitude progression analysis of both groups showed that the origin of focus of the hypsarrhythmic pattern is from the occipital region while no such pattern has been noticed in the control group. Discussion and conclusion Hypsarrythmia is known to show multifocal origin. Predominant occipital origin in older age group subjects distinguishes the condition from classical hypsarrythmia of early childhood. The occipital origin may be indicative of persistent immaturity of the thalamocortical synaptic pathway.

Effects of the COVID-19 pandemic on semen quality in male partners of infertile couples: a hospital-based observational study.

The effects of the coronavirus disease 2019 (COVID-19) pandemic on male fertility have received considerable attention because human testes contain high levels of angiotensin-converting enzyme-2 receptors, through which severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can enter. Early studies showed decreases in semen quality during and after recovery from COVID-19. However, no semen quality studies have examined the effects of widespread subclinical and mild disease, as well as changes in lifestyle, psychosocial behavior, intake of dietary supplements, and stress. This cross-sectional study compared semen quality parameters in male partners of infertile couples between men who underwent semen analysis before the COVID-19 pandemic (prepandemic group) and men who underwent semen analysis during the pandemic period (pandemic group); the analysis sought to clarify the overall effects of the pandemic. No participants in the pandemic group had experienced clinically overt disease. Among the 239 participants, mean body weight (P = 0.001), mean body mass index (P < 0.001), median sperm concentration (P = 0.014), total sperm count (P = 0.006), and total percentages of motile (P = 0.013) and abnormal cells (P < 0.001) were significantly greater in the pandemic group (n = 137) than those in the prepandemic group (n = 102). Among abnormal cells, the percentages of cells with excess residual cytoplasm (P < 0.001), head defects (P < 0.001), and tail defects (P = 0.015) were significantly greater in the pandemic group than those in the prepandemic group. With the exception of morphology, the overall semenogram results were better in the pandemic group than those in the prepandemic group.

Role of cystatin C and calprotectin as potential early prognostic biomarkers in COVID-19 patients admitted to a dedicated COVID care facility.

Background: The COVID-19 pandemic has claimed millions of lives. A tool for early prediction of severity and mortality risk is desirable for better utilization of health care facilities. Several biomarkers like D-dimer, lactate dehydrogenase (LDH), C-reactive protein (CRP) and some recently explored biomarkers like serum cystatin C and serum calprotectin have been proposed as prognostic markers of COVID-19, but their role as prognostic markers is so far undefined. The present work attempted to investigate the possible role of serum cystatin C and serum calprotectin as prognostic tools to predict severity and outcome ahead of time. Material and Methods: This observational cohort study was carried out on 95 COVID-19 patients admitted to a dedicated COVID care facility from mid-October 2020 to January 2021. Serial estimations of serum cystatin C and serum calprotectin levels were done and assessed for significant difference between severe (NEWS 2 score ≥5) and non-severe (NEWS 2 score <5) groups, survivors and deceased and on the basis of comorbidities at each time points. Survival analysis was done based on the optimal thresholds for severity and mortality, calculated from the receiver operating characteristic (ROC). Result: The results showed that median cystatin C levels were significantly higher on the first day in the severe group (P < 0.001) and in patients with cardiovascular disease (P < 0.05), chronic lung disease (P = 0.009) and among patients who died (P < 0.05). It remained raised on day 3 in severe (P < 0.05) and deceased (P < 0.05) group. Serum calprotectin levels were significantly higher in patients with chronic lung disease (P = 0.008) and in those who died (P < 0.05). Conclusion: Serum cystatin C could be used as a tool for early prognosis and therapeutic decision-making for COVID-19 patients. Serum calprotectin seems to be a better marker of critical illness.