Review and experimental verification of x-ray dark-field signal interpretations with respect to quantitative isotropic and anisotropic dark-field computed tomography.

Talbot(-Lau) interferometric x-ray and neutron dark-field imaging has, over the past decade, gained substantial interest for its ability to provide insights into a sample's microstructure below the imaging resolution by means of ultra small angle scattering effects. Quantitative interpretations of such images depend on models of the signal origination process that relate the observable image contrast to underlying physical processes. A review of such models is given here and their relation to the wave optical derivations by Yashiro et al and Lynch et al as well as to small angle scattering is discussed. Fresnel scaling is introduced to explain the characteristic distance dependence observed in cone beam geometries. Moreover, a model describing the anisotropic signals of fibrous objects is derived. The Yashiro-Lynch model is experimentally verified both in radiographic and tomographic imaging in a monochromatic synchrotron setting, considering both the effects of material and positional dependence of the resulting dark-field contrast. The effect of varying sample-detector distance on the dark-field signal is shown to be non-negligible for tomographic imaging, yet can be largely compensated for by symmetric acquisition trajectories. The derived orientation dependence of the dark-field contrast of fibrous materials both with respect to variations in autocorrelation width and scattering cross section is experimentally validated using carbon fiber reinforced rods.

Finding robust descriptive features for the characterization of the coarsening dynamics of three dimensional whey protein foams.

HYPOTHESIS: Understanding the coarsening behavior of foams is of great interest for their deliberate design. In order to systematically quantify the influence of surfactants and other chemical parameters, identifying robust descriptive features of observed foam aging dynamics is essential. Existing coarsening theories for both wet and dry foams provide concise models with respective descriptive parameters. EXPERIMENT: Multiple micro computed tomography scans of moderately wet polydisperse ß-Lactoglobulin foam are recorded over a period of 15min. The growth behavior of a large fraction of about 5×10(4) pores that constitute the imaged volume is individually observed and statistically analyzed as a function of pore radius as well as number of neighboring pores. FINDINGS: The three-dimensional analog of von Neumann's law for dry foams by Glazier is confirmed as a suiting empirical model, whereby a critical number of 13±7 neighbors and a diffusion coefficient of (1.8±0.8)×10(-11)m(2)/s are found for an exemplary sample. The pores growth can as well be related to their radius by means of Lemlich's coarsening model for wet foams though, whereby a critical radius marking the transition between shrinkage and growths is found to be Rc=(300±85)µm. Although different, both models fit similarly well given the broad variance of the observed growth rates.

Quantitative studies on inner interfaces in conical metal joints using hard x-ray inline phase contrast radiography.

Quantitative investigation of micrometer and submicrometer gaps between joining metal surfaces is applied to conical plug-socket connections in dental titanium implants. Microgaps of widths well beyond the resolving power of industrial x-ray systems are imaged by synchrotron phase contrast radiography. Furthermore, by using an analytical model for the relatively simple sample geometry and applying it to numerical forward simulations of the optical Fresnel propagation, we show that quantitative measurements of the microgap width down to 0.1 µm are possible. Image data recorded at the BAMline (BESSY-II light source, Germany) are presented, with the resolving power of the imaging system being 4 µm in absorption mode and ∼14 µm in phase contrast mode (z(2)=0.74 m). Thus, phase contrast radiography, combined with numerical forward simulations, is capable of measuring the widths of gaps that are two orders of magnitude thinner than the conventional detection limit.

On the possibilities of hard X-ray imaging with high spatio-temporal resolution using polychromatic synchrotron radiation.

Time-resolved imaging with penetrating radiation has an outstanding scientific value but its realisation requires a high density of photons as well as corresponding fast X-ray image detection schemes. Bending magnets and insertion devices of third generation synchrotron light sources offer a polychromatic photon flux density which is high enough to perform hard X-ray imaging with a spatio-temporal resolution up to the µm-µs range. Existing indirect X-ray image detectors commonly used at synchrotron light sources can be adapted for fast image acquisition by employing CMOS-based digital high speed cameras already available on the market. Selected applications from life sciences and materials research underline the high potential of this high-speed hard X-ray microimaging approach.

Moisture changes in the plant cell wall force cellulose crystallites to deform.

Nano-crystallite deformation of cellulose microfibrils in the secondary cell wall layer of spruce wood tracheids was observed during de- and rehydration experiments below the fibre saturation point. A quantitative analysis of the (004), (200) and the (110)/(11 0) doublet X-ray diffraction peaks revealed longitudinal contraction, lateral expansion and changes in the monoclinic angle of the cellulose unit cell during drying of wood fibres. Experiments on unfixed samples as well as the simultaneous application of mechanical tensile and dehydration stress to samples hold at constant length showed two deformation mechanisms of different nature and magnitude. The first mechanism depends on the relative wood moisture content and the second one on the macroscopic tensile stress. These findings imply a new perspective on the role of water adsorption perceiving a hydration-induced structural change of cellulose crystal structure as a major driving force for deformation.

In vitro synchrotron-based radiography of micro-gap formation at the implant-abutment interface of two-piece dental implants.

Micro-gap formation at the implant-abutment interface of two-piece dental implants was investigated in vitro using high-resolution radiography in combination with hard X-ray synchrotron radiation. Images were taken with the specimen under different mechanical loads of up to 100 N. The aim of this investigation was to prove the existence of micro-gaps for implants with conical connections as well as to study the mechanical behavior of the mating zone of conical implants during loading. Synchrotron-based radiography in comparison with classical laboratory radiography yields high spatial resolution in combination with high contrast even when exploiting micro-sized features in highly attenuating objects. The first illustration of a micro-gap which was previously indistinguishable by laboratory methods underlines that the complex micro-mechanical behavior of implants requires further in vitro investigations where synchrotron-based micro-imaging is one of the prerequisites.

Fresnel-propagated imaging for the study of human tooth dentin by partially coherent x-ray tomography.

Recent methods of phase imaging in x-ray tomography allow the visualization of features that are not resolved in conventional absorption microtomography. Of these, the relatively simple setup needed to produce Fresnel-propagated tomograms appears to be well suited to probe tooth-dentin where composition as well as microstructure vary in a graded manner. By adapting analytical propagation approximations we provide predictions of the form of the interference patterns in the 3D images, which we compare to numerical simulations as well as data obtained from measurements of water immersed samples. Our observations reveal details of the tubular structure of dentin, and may be evaluated similarly to conventional absorption tomograms. We believe this exemplifies the power of Fresnel-propagated imaging as a form of 3D microscopy, well suited to quantify gradual microstructural-variations in teeth and similar tissues.