Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Animal ; 12(1): 76-87, 2018 Jan.
Article in English | MEDLINE | ID: mdl-28689516

ABSTRACT

The main objectives of the study were to: (1) deeply analyse the serum protein composition of Equus caballus, (2) assess the effectiveness of the high-abundant protein depletion and improve the concentration of medium- and low-abundant proteins. The analysis were performed on the blood plasma of three healthy part-Arabian mares. The implementation of two-dimensional electrophoresis and matrix-assisted laser desorption/ionisation - time of flight mass spectrometry allowed us to establish a horse plasma proteome map. Serum proteins were resolved at pH 4 to 7, followed by 12% SDS-PAGE. As a result 136 spots were successfully identified, representing the products of 46 unique genes. Of these, 22 gene products have not been previously identified in horse serum/plasma samples using proteomic tools. Gene ontology analysis showed that almost 30% of all identified gene products belong to the coagulation and complement cascades. These results can undoubtedly serve as a useful and prospective prerequisite for the future analysis of horse plasma proteome changes in different physiological and pathophysiological conditions. The use of the medium- and low-abundant protein enrichment tool increased their abundance and allowed us to identify a higher number of protein gene products. The highest depletion efficiency was observed for the most abundant plasma proteins, that is albumin, IgG heavy chains and serotransferrin.


Subject(s)
Blood Proteins/analysis , Horses/blood , Proteome , Proteomics , Animals , Biomarkers/blood , Electrophoresis, Gel, Two-Dimensional/veterinary , Female , Prospective Studies , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/veterinary
2.
Reprod Domest Anim ; 44(3): 540-51, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19055561

ABSTRACT

The paper reviews the various factors affecting dystocia in cattle. It is based mainly on the recent studies found in the literature of the subject but refers occasionally to some older papers as well. The factors are grouped into four main categories: direct factors, phenotypic factors related to calf and cow, non-genetic and genetic factors. The first group includes malpresentations and uterine torsion. The second one includes: calf birth weight, multiple calvings, perinatal mortality, cow pelvic area, cow body weight and body condition at calving, gestation length. The non-genetic factors are: cow age and parity, year and season of calving, place of calving, maintenance practises, disorders, calf sex and nutrition. Other non-genetic factors are the level of hormones in the periparturient period, in vitro production of embryos and embryo cloning. Finally, the genotypes of cow, bull and calf, inbreeding, muscular hypertrophy, selection and quantitative trait loci form the fourth group of genetic factors.


Subject(s)
Cattle Diseases , Dystocia/veterinary , Animals , Birth Weight , Body Composition , Body Weight , Cattle , Cattle Diseases/etiology , Cattle Diseases/genetics , Dystocia/etiology , Dystocia/genetics , Female , Gestational Age , Labor Presentation , Male , Parity , Perinatal Mortality , Phenotype , Pregnancy , Reproductive Techniques/veterinary , Torsion Abnormality , Uterine Diseases
SELECTION OF CITATIONS
SEARCH DETAIL
...