Mitochondrial dysfunction route as a possible biomarker and therapy target for human cancer.

Mitochondria are vital organelles found within living cells and have signalling, biosynthetic, and bioenergetic functions. Mitochondria play a crucial role in metabolic reprogramming, which is a characteristic of cancer cells and allows them to assure a steady supply of proteins, nucleotides, and lipids to enable rapid proliferation and development. Their dysregulated activities have been associated with the growth and metastasis of different kinds of human cancer, particularly ovarian carcinoma. In this review, we briefly demonstrated the modified mitochondrial function in cancer, including mutations in mtDNA, reactive oxygen species production, dynamics, apoptosis of cells, autophagy, and calcium excess to maintain cancer genesis, progression, and metastasis. Furthermore, the mitochondrial dysfunction pathway for some genomic, proteomic, and metabolomics modifications in ovarian cancer has been studied. Additionally, ovarian cancer has been linked to targeted therapies and biomarkers found through various alteration processes underlying mitochondrial dysfunction, notably targeting reactive oxygen species, metabolites, rewind metabolic pathways, and chemo-resistant ovarian carcinoma cells.

Multifunctional Ag2O/chitosan nanocomposites synthesized via sol-gel with enhanced antimicrobial, and antioxidant properties: A novel food packaging material.

In this study, a single step in situ sol-gel method was used to syntheses nanocomposite films using chitosan (CS) as the basis material, with the addition of silver oxide nanoparticles (Ag2O) at several weight percentages (5 %, 10 %, and 15 % Ag2O/CS). The structural characteristics of Ag2O/CS films were investigated using a range of analytical techniques. The presence of the primary distinctive peaks of chitosan was verified using FTIR spectra analysis. However, a minor displacement was observed in these peaks due to the chemical interaction occurring with silver oxide molecules. XRD analysis demonstrated a significant increase in the crystallinity of chitosan when it interacted with metal oxide nanoparticles. Furthermore, it is believed that the interaction between silver oxide and the active binding sites of chitosan is responsible for the evenly dispersed clusters shown in the micrographs of the chitosan surface, as well as the random aggregations within the pores. EDS technique successfully identified the presence of distinctive silver signals within the nanocomposite material, indicating the successful absorption of silver into the surface of the polymer. The developed Ag2O/CS nanocomposite showed promising antibacterial activity against Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis and Staphylococcus aureus). Also, Ag2O/CS nanocomposite exhibited marked antifungal activity against Candida albicans, Aspergillus flavus, A. fumigatus, A. niger, and Penicillium chrysogenum. The antioxidant activity of the developed nanocomposite films was studied by ABTS radical scavenging. The highest antioxidant and antibacterial properties were achieved by including 15 % silver oxide into the chitosan. Therefore, our finding indicate that chitosan­silver oxide nanocomposites exhibits significant potential as a viable material for application in several sectors of the food packaging industry.

Essential Oil of Foeniculum vulgare Mill. as a Green Fungicide and Defense-Inducing Agent against Fusarium Root Rot Disease in Vicia faba L.

Fusarium solani, the causative agent of root rot disease is one of the major constraints of faba bean (Vicia faba L.) yield worldwide. Essential oils have become excellent plant growth stimulators besides their antifungal properties. Foeniculum vulgare Mill. (fennel) is a familiar medicinal plant that has inhibitory effects against phytopathogenic fungi. Herein, different concentrations of fennel seed essential oil (FSEO) (12.5, 25, 50, 100, 200 and 400 µL/mL) were examined against F. solani KHA10 (accession number MW444555) isolated from rotted roots of faba bean in vitro and in vivo. The chemical composition of FSEO, through gas chromatography/mass spectroscopy, revealed 10 major compounds. In vitro, FSEO inhibited F. solani with a minimum inhibitory concentration (MIC) of 25 µL/mL. In vivo, FSEO suppressed Fusarium root rot disease in Vicia faba L. by decreasing the disease severity (61.2%) and disease incidence (50%), and acted as protective agent (32.5%) of Vicia faba L. Improvements in morphological and biochemical parameters were recorded in FSEO-treated faba seeds. Moreover, the expression level of the defense-related genes defensin and chitinase was noticeably enhanced in treated plants. This study suggested using FSEO as a promising antifungal agent against F. solani not only to control root rot disease but also to enhance plant growth and activate plant defense.

Evaluation of cytokeratin-1 in the diagnosis of hepatocellular carcinoma.

BACKGROUND: This study was undertaken to investigate whether serum cytokeratin-1 (CK1) could complement alpha-fetoprotein (AFP) to improve the diagnosis of hepatocellular carcinoma (HCC). METHODS: CK1 was identified using western blot and ELISA in serum samples from 250 Egyptian patients including 150 with HCC, 100 with liver cirrhosis (LC) and 50 healthy controls. Multivariate discriminant analysis (MDA) and ROC curve analyses were used to create a predictive model including CK1 in addition to a panel of routine blood markers. RESULTS: CK1 was identified at 67 kDa and quantified in sera of HCC patients using western blot and ELISA. MDA selected a score for the prediction of HCC from LC patients based on levels of CK1, albumin and AFP. An area under the ROC curves (AUC) of the score was 0.87. The score showed a sensitivity of 87% vs 39% sensitivity of AFP at cutoff value of 200 IU/ml for prediction HCC. Absolute specificity (100%) was obtained to discriminate HCC from healthy individuals. CONCLUSIONS: This study suggests that the use of a combination of score including CK1, AFP and albumin in clinical practice provides a non invasive and simple test that could increase significantly the sensitivity of HCC diagnosis.