ABSTRACT
Acute toxicity of silver nanoparticle (AgNP) for photosynthesis in Chlamydomonas reinhardtii was studied using an M-PEA2 fluorimeter. Analysis of the fluorescence induction curves in the presence of AgNP at low concentrations revealed inhibited electron transport in the PS2 photosystem and increased content of Q(B)-nonreducing centers. No direct effect of AgNP on the reactions of P700 oxidation in PS1 was found, while energization of the photosynthetic membranes was affected. Investigation of the parameters of the prompt and delayed fluorescence is proposed as a method for early detection of AgNP in the environment.
Subject(s)
Chlamydomonas reinhardtii/drug effects , Metal Nanoparticles/toxicity , Silver/toxicity , Chlamydomonas reinhardtii/metabolism , Chlorophyll , Dose-Response Relationship, Drug , Electron Transport/drug effects , Fluorescence , Photosystem II Protein Complex/metabolism , Toxicity Tests, AcuteABSTRACT
Two cyanobacterial cultures from rice paddies of Kyzylorda region, Kazakhstan were isolated and characterized: Anabaena variabilis and Nostoc calsicola. Based on these cultures, new consortia ofcyanobacteria, microalgae and Azotobacter were developed: ZOB-1 (Anabaena variabilis, Chlorella vulgaris, and Azotobacter sp.) and ZBOB-2 (Nostoc calsicola, Chlorella vulgaris, and Azotobacter sp.). High growth rate and photosynthetic activity of microalgae were observed in these consortia. The active consotrium ZOB-1 was selected, which improvd germination and growth of rice plants. ZOB-1 was recommended as a biostimulator and biofertilizer for crops.
