ABSTRACT
<b>Background and Objective:</b> The proposed study involves the approach from the point of anti-viral activity of gold nanoparticles against the <i>Bluetongue virus</i>. Among viral diseases, Bluetongue is regarded as an economically scouring disease. Neither a vaccine nor an antiviral drug is available for the prevention or treatment of this disease. The antiviral activity of gold nanoparticles synthesized by a novel isolate of <i>Streptomyces tuirus</i> DBZ39 is the breakthrough of the study. <i>Streptomyces tuirus </i>DBZ39, a novel isolate obtained from alkaline soil was proved to be efficient actinomycetes, for the extracellular synthesis of gold nanoparticles. <b>Materials and Methods:</b> An upstream bioprocess was optimized and developed for the synthesis of controlled size gold nanoparticles with solitary mono dispersal pattern in aurum chloride solution. The characterization and confirmation of gold nanoparticles were illustrated by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Analysis (EDAX) and Fourier Transmission Infrared Radiation Analysis (FTIR). <b>Results:</b> Biomass size of 3 g, substrate concentration of 1 mM, pH of 8.5 and temperature of 45°C were observed as optimum conditions for the synthesis of 15-24 nm size gold nanoparticles. The <i>Bluetongue virus</i> (BTV) which belongs to the genus Orbivirus in the family Reoviridae with 26 serotypes is an etiological agent of infectious and non-contagious Bluetongue disease of main sheep and several other domestic animals. <b>Conclusion:</b> Gold nanoparticles for the 1st time, at a higher concentration of 1:64 dilutions revealed a very promising and novel antiviral property against the <i>Bluetongue virus</i>.
Subject(s)
Antiviral Agents/standards , Bluetongue virus/drug effects , Gold/pharmacology , Streptomyces/isolation & purification , Antiviral Agents/pharmacology , Bluetongue/drug therapy , Bluetongue/physiopathology , Bluetongue virus/pathogenicity , Gold/therapeutic use , Nanoparticles/standards , Streptomyces/metabolismABSTRACT
BACKGROUND AND OBJECTIVE: Nanotechnology is a cumbersome field used in industrial, medical and environmental applications. Abundant information regarding the genotoxicity of gold nanoparticles is available, but limited information is available about the genotoxicity of enzymes and bioconjugates of gold nanoparticles from microbial sources. The present investigation reveals the genotoxicity of bioconjugate of tyrosinase and gold nanoparticles from Streptomyces sp. MATERIALS AND METHODS: Tyrosinase and gold nanoparticles were isolated from Streptomyces tuirus DBZ39 and employed for the development of bioconjugates by the flocculation assay method. Methyl thiazole (MTT) assay, transmission electron microscopy, Dynamic Light Scattering (DLS) and UV-vis absorption spectroscopy were used for the investigation. RESULTS: Bioconjugate showed a significant genotoxic effect at a concentration of 18 µL and at least 10 µL. Bioconjugate of tyrosinase and gold nanoparticles by Streptomyces tuirus DBZ39 revealed a more expressed genotoxic effect than nanoparticles alone. The efficacy of gold nanoparticles was expected to improve the bonding of tyrosine due to their catalytic properties. CONCLUSION: This innovative concept of the application of Tyr-AuNps bioconjugate can be used for the development of powerful weapons in medicinal fields. The introduction of bioconjugates of enzymes and nanoparticles in the field of drug design and cancer imaging will be a fundamental breakthrough in medicine.
Subject(s)
Bone Marrow/drug effects , DNA Damage , Gold/toxicity , Lymphocytes/drug effects , Metal Nanoparticles/toxicity , Monophenol Monooxygenase/toxicity , Mutagenicity Tests , Streptomyces/enzymology , Animals , Bone Marrow/pathology , Cells, Cultured , Gold/metabolism , Humans , Lymphocytes/pathology , Mice , Monophenol Monooxygenase/genetics , Monophenol Monooxygenase/metabolism , Nanotechnology , Streptomyces/geneticsABSTRACT
Most of the phenol compounds are toxic and have been considered as hazardous pollutants. Several physicochemical and biological methods are available to detect and monitor the phenol pollutants in water and soil. In the present study, phenol constituents of winery, paper and plastic industrial effluents were successfully detected employing tyrosinase-gold nanoparticles bioconjugate. The synthesis of extracellular tyrosinase and gold nanoparticles was achieved by a single isolate of Streptomyces sp. DBZ-39. Enhanced production (369.41 IU) of tyrosinase was produced in submerged bioprocess employing response surface method with central composite design. Extracellular gold nanoparticles synthesized (12-18 nm) by Streptomyces sp. DBZ-39 were characterized with TEM, EDAX and FTIR analysis. A rapid detection (within 10 min) of phenol constituents from winery effluents was achieved by bioconjugate, when compared to tyrosinases and gold nanoparticles independently. Streptomyces tyrosinase could exhibit relatively a better performance than commercially available mushroom tyrosinase in the detection of phenol constituents. Winery effluent has shown much higher content (0.98 O.D) of phenol constituents than paper and plastic effluents based on the intensity of color and U.V absorption spectra.
