ABSTRACT
The efficiency of application of test-system "Antibiotic Choice" was examined concerning evaluation of sensitivity to medications of maximal possible number of bacteria from pathological samples at burn trauma without isolation of pure culture. The results of metagenome analysis demonstrated that test-system permits supporting factually all bacteria discovered in wound discharge, including ones related to not cultivated yet. The comparison was carried out concerning results of standard identification of sensitivity of bacteria to antibiotics and efficient medication according the results of application of test-system "Antibiotic Choice". The obtained results demonstrate that test-system permits choosing antibiotic during 6-20 hours without separation of pure culture.
ABSTRACT
The effectiveness of application of test-system "Choice of antibiotic" was evaluated as a tool for incubation of maximal amount of bacteria from pathological material under acute cystitis. The results of meta-genome analysis established that test-system permits supporting growth of practically all bacteria detected in urine, including ones relating to "uncultivated for the present". The comparison of results of standard detection of sensitivity of bacteria to antibiotics and identification of effective pharmaceutical according the results of application of test-system "Choice of antibiotic" as well was implemented It is demonstrated that test- system permits choosing antibiotic during 6-20 hours wiihout isolation of pure strain.
Subject(s)
Anti-Bacterial Agents/pharmacology , Cystitis/drug therapy , Diagnostic Tests, Routine/methods , Enterobacteriaceae Infections/drug therapy , Enterobacteriaceae/drug effects , Urinary Tract Infections/drug therapy , Acute Disease , Anti-Bacterial Agents/classification , Cystitis/diagnosis , Cystitis/microbiology , Enterobacteriaceae/classification , Enterobacteriaceae/growth & development , Enterobacteriaceae Infections/diagnosis , Enterobacteriaceae Infections/microbiology , High-Throughput Screening Assays , Humans , Metagenome , Microbial Sensitivity Tests , Urinary Tract Infections/diagnosis , Urinary Tract Infections/microbiology , Urogenital System/drug effects , Urogenital System/microbiologyABSTRACT
The present analysis of blood traces on the clothes of the alleged criminals is based on the results of 109 medical criminalistic expertises. They are illustrated by examples from practical work.
Subject(s)
Blood Stains , Clothing , Crime Victims , Criminals , Forensic Medicine/methods , Homicide/legislation & jurisprudence , Blood Grouping and Crossmatching , Clothing/standards , Criminals/legislation & jurisprudence , HumansABSTRACT
Peptide CH3CO-Lys-Lys-Arg-Arg-NH2 (protectin) was synthesized and its activity was studied on the model of experimental myocardial infarction in rats in comparison to the reference antihypoxant drug riboxin. Intranasal injections ofprotectin at doses within 2-20 microg/kg once a day by course of 7 days produced a pronounced anti-ischemic action, improved coronary circulation of the blood, increases contractile activity of myocardium, reduced intensity of lipid peroxidation, and improved antioxidant protection. In some respects (improved coronary circulation of the blood, increased antioxidant protection), protectin was more effective than riboxin.
Subject(s)
Inosine Diphosphate/administration & dosage , Myocardial Infarction/drug therapy , Oligopeptides , Administration, Intranasal , Animals , Antioxidants/metabolism , Coronary Circulation/drug effects , Disease Models, Animal , Dose-Response Relationship, Drug , Lipid Peroxidation/drug effects , Male , Oligopeptides/administration & dosage , Oligopeptides/chemical synthesis , Rats , Rats, Sprague-DawleyABSTRACT
In order to create effective therapeutically significant oligonucleotide structures, a series of analogs of thrombin-binding aptamer d(GGTTGGTGTGGTTGG) containing thiophosphoryl substitutions were synthesized. The anticoagulation effects of the resultant thrombin-binding aptamer analogs were evaluated and the effects of local thiomodifications on their structure and function were studied, including the effects on stability in blood plasma and resistance to DNA nucleases. A promising modified oligonucleotide (LL11) was found with the structure modified only in TT loops. It retains antithrombin properties of thrombin-binding aptamer, but is more resistant to biodegradation.
Subject(s)
Anticoagulants/metabolism , Aptamers, Nucleotide/blood , Aptamers, Nucleotide/metabolism , Oligonucleotides/metabolism , Thrombin/metabolism , Anticoagulants/pharmacology , Aptamers, Nucleotide/genetics , Base Sequence , Chromatography, High Pressure Liquid , Mass Spectrometry , Molecular Sequence Data , Oligonucleotides/pharmacology , Spectrophotometry, Ultraviolet , Thrombin TimeABSTRACT
Analysis of the use of real-time PCR with fluorescent registration of results for gene diagnosis of infectious diseases showed that the sensitivity and reliability of quantitative evaluation of DNA targets directly depended on the method of purification of oligonucleotide probes. Chromatographic behavior of synthetic probes carrying various fluorophores and fluorescence quenchers was analyzed. Approaches to optimization of purification methods are proposed enabling elimination of previously undetectable admixtures. The importance of these studies is explained by the need in extending the armory of methods for the development and production of diagnosticums for detection of infectious and hereditary diseases, identification of genetically modified organisms, and for a wide spectrum of research in molecular biology and medicine.
Subject(s)
Oligonucleotide Probes/isolation & purification , Polymerase Chain Reaction/methods , Chromatography, High Pressure Liquid/methods , DNA/analysis , HumansABSTRACT
The prostaglandin E sulprostone was studied in experiments on pregnant rats. It was shown to stimulate the bioelectrical activity of the uterus, by increasing the amplitude and frequency of biological potentials. Sulprostone was found to have abortive and embryotoxic effects which were especially pronounced during placentation. The drug induced a slight teratogenic effect.
