ABSTRACT
According to some studies, two-thirds of the world's plant species have therapeutic worth. Saurauia vulcani (Korth.) is one of them. This is something that can be found in Indonesia. Traditionally, this herb was known as pirdot and was used to cure diabetes. This article examines the scientific activities of pirdot leaves as well as their safety for usage. This study used a literature review article as its design. Searching for related publications using data from Pubmed, Proquest, Ebsco, ScienceDirect, and Google Scholar for the last ten years (2011-2021) yielded 141 articles. There are 14 pertinent articles that explore their substance and application in health. The content of secondary metabolites that have been reported proves that pirdot has properties as an antidiabetic, immunostimulant, antidiarrhoea, anticholesterol, and hepatoprotective.
ABSTRACT
Cellulose nanofibers (CNFs), chitosan, and silver nanoparticles (AgNPs) are widely used to enhance the active functions and antibacterial properties of wound dressings. This study was conducted to prepare CNF/AgNP-chitosan using a straight incorporation method and to assess its antimicrobial activity. CNFs were isolated from oil palm empty fruit bunches (OPEFBs) using the pulping method and acid hydrolysis. AgNPs were synthesized using a green synthesis method. The wound dressing was produced by mixing a 10% CNF solution in LiCl/DMAc and AgNP-chitosan in a glass plate with various ratios of CNF/AgNP-chitosan, i.e., 100:0, 80:20, 60:40, and 50:50. UV-visible and TEM analyses were conducted to confirm the formation of AgNPs and CNFs at the nanoscale. The results showed particles with an absorption wavelength of 435 nm and spherical shapes. Based on the calculation using ImageJ software, the diameters of CNFs were approximately 50 nm, and the lengths were several micrometers. FTIR was used to analyze the chemical bonding of AgNP-chitosan and the incorporated AgNP-chitosan in CNFs. Based on the XRD analysis, the presence of AgNPs did not affect the crystallinity of the CNFs. SEM images showed that the addition of AgNPs resulted in the stretching of CNF pores on the pads. Thermal degradation of the film increased with the addition of AgNP-chitosan by up to 40%. Antimicrobial tests and hemocompatibility tests showed that the formed CNF/AgNP-chitosan film successfully inhibited bacterial growth and was classified as a nonhemolytic material; thus, its potential as a wound dressing should be further studied.
ABSTRACT
Aim Vulvovaginal candidiasis (VVC) is a disease mostly caused by Candida albicans and affects the quality of life of women especially in the form of chronic recurrent vulvovaginal candidiasis(RVVC). Nigella sativa is known to have several effects such as antimicrobial, anti-inflammatory, immune stimulation and anticancer properties. The aim of this study was to evaluate the effect of Nigella sativa on vulvovaginal candidiasis. Methods This study is a true experimental design, we used 28 Wistar strain rats divided into 4 groups, all groups were conditioned in a pseudoestrus state. Candida albicans was inoculated into the rats' vagina for 3 consecutive days. All groups were observed every 24 hours, 48 hours and 72 hours to evaluate the number of Candida albicans colonies, IgG and IgM anti Candida. Results After administration of intervention, there was a significant difference in the amount of fungal colonization after the treatment in each group (p<0.001). There was no significant difference in the effectiveness of Nigella sativa and fluconazole in supressing Candida albicans colonies after 72 hours (p=0.101). The administration of Nigella sativa showed a significant difference in the increase in IgM levels compared to the others group (p<0.001), while the IgG level did not show a significant difference (p=0.423). Conclusion Nigella sativa provides a therapeutic effect by decreasing the number of fungal colonies and increasing IgM levels.
Subject(s)
Candidiasis, Vulvovaginal , Nigella sativa , Animals , Antifungal Agents/pharmacology , Antifungal Agents/therapeutic use , Candida albicans , Candidiasis, Vulvovaginal/drug therapy , Female , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Quality of Life , Rats , Rats, WistarABSTRACT
Objectives: Plectranthus amboinicus (Lour.) Spreng. is a plant which has the potential as an anti-cancer agent. To enhance the bioavailability of an extract, it is necessary to transform the extract into nanoparticles. This research aimed to create nanoparticles of the extract and investigate the anti-proliferative and pro-apoptosis effects on T47D breast cancer cell lines. Materials and Methods: The extraction with ethanol was performed using maceration method. The nanoparticles were prepared by using the ionic gelation method. Cytotoxic assay method evaluation of the proliferation of T47D cell line (using doubling time) was carried out using the MTT assay. Apoptosis was observed using the flow cytometry assay. Results: Treatment with Plectranthus amboinicus (Lour.) Spreng. ethanolic extract nanoparticles (PAEEN) inhibited the proliferation of T47D cell lines after 48 hours and 72 hours of incubation at concentrations of 22.3 µg/mL, 44.6 µg/mL, and 89.2 µg/mL. The viable cells were 93%, 86%, 54% (48 hours), and 98%, 71%, 57% (72 hours), respectively. The nanoparticles of extract also induced apoptosis at concentrations of » IC50 (2.16%), ½ IC50 (1.57%), and IC50 (2.43%). Conclusion: PAEEN exhibits the anti-proliferative effect on T47D breast cancer cells via apoptosis. Further study is required to confirm the mechanism of PAEEN in the cell cycle arrest and apoptosis induction on T47D cells.
