ABSTRACT
Establishing the whole set of aneuploids, for one naturally evolved allopolyploid species, provides a unique opportunity to elucidate the transcriptomic response of the constituent subgenomes to serial aneuploidy. Previously, the whole set of monosomic alien addition lines (MAALs, C1-C9) with each of the nine C subgenome chromosomes, added to the extracted A subgenome, was developed in the context of the allotetraploid Brassica napus donor "Oro," after the restitution of the ancestral B. rapa (RBR Oro) was realized. Herein, transcriptomic analysis using high-throughput technology was conducted to detect gene expression alterations in these MAALs and RBR. Compared to diploid RBR, the genes of all of the MAALs showed various degrees of dysregulated expressions that resulted from cis effects and more prevailing trans effects. In addition, the trans-effect on gene expression in MAALs increased with higher levels of homology between the recipient A subgenome and additional C subgenome chromosomes, instead of gene numbers of extra chromosomes. A total of 10 trans-effect dysregulated genes, among all pairwise comparisons, were mainly involved in the function of transporter activity. Furthermore, highly expressed genes were more prone to downregulation and vice-versa, suggesting a common trend for transcriptional pattern responses to aneuploidy. These results provided a comprehensive insight of the impact of gene expression of individual chromosomes, in one subgenome, on another intact subgenome for one allopolyploid with a long evolutionary history.
ABSTRACT
ObjectiveTo observe the clinical efficacy of moxibustion plus acupoint autohemotherapy in treating allergic rhinitis due to lung-spleen qi deficiency.MethodTotally 120 eligible subjects were divided by using the random number table into a comprehensive group, a moxibustion group and a Western medication group. The comprehensive group was intervened by moxibustion plus acupoint autohemotherapy, the moxibustion group was by moxibustion,and the Western medication group was by Loratadine tablets. The acupoint autohemotherapy was give twice a week and the rest treatments were given once a day, 7 das a course, for 4 courses in total. A follow-up study was conducted 3 months later. The clinic efficacy was evaluated before and after intervention, as well as in the follow-up study.ResultThe three groups all achieved significant short-term efficacies after intervention, and the comprehensive group was equivalent to the moxibustion group, bothsuperior to the Western medication group(P<0.05). According to the follow-up study, the long-term efficacies of the comprehensive group and moxibustion group were both significantly higher than that of the Western medication group (P<0.01,P<0.05), and the moxibustion group was superior to the comprehensive group in comparing the long-term efficacy (P<0.01).ConclusionMoxibustion plus acupoint autohemotherapy and dry moxibustion both can produce significant short-term and long-term therapeutic efficacies in treating allergic rhinitis due to lung-spleen qi deficiency. The long-term efficacy of moxibustion is higher than that of moxibustion plus acupoint autohemotherapy in treating allergic rhinitis due to lung-spleen qi deficiency. Acupoint autohemotherapy requires strict aseptic operation, which restricts its application in family healthcare. Long-term use of moxibustion can activate yang qi, and thus plays a role in preventing diseases.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of moxibustion combined with autohemotherapy of acupoint injection and simple moxibustion for allergic rhinitis(AR) withdeficiency of lung and spleen and to explore its mechanism.</p><p><b>METHODS</b>By random number table,75 patients who matched the criteria were assigned into a moxibustion combined with autohemotherapy of acupoint injection group(a combination group,37 cases) and a moxibustion group(38 cases). In the combination group,moxibustion was used at bilateral Yingxiang(LI 20) and injection bila-teral Feishu(BL 13),Pishu(BL 20) and Zusanli(ST 36). In the moxibustion group,moxibustion was applied bilaterally at all the above acupoints. Acupoint injection was given twice a week,and moxibustion once a day,seven days as a course. The patients were observed for four courses and followed up half a year after treatment. Rhinoconjunctivitis quality of life questionnaire(RQLQ) was compared before and after treatment and at follow-up. The short-term and long-term effects as well as the changes of interferon-(IFN-),interleukin-4(IL-4) and immunoglobulin E(IgE) were observed in the two group.</p><p><b>RESULTS</b>After treatment,the obviously effective rate of the combination group was 75.68%(28/37),which was higher than 52.63%(20/38) of the moxibustion group(<0.05). But at follow-up, the obviously effective rate of the moxibustion group was 47.37%(18/38),which was better than 24.32%(9/37) of the combination group(<0.05). Life quality of the two group in all aspects after treatment and at follow-up were apparently improved compared with the baseline(<0.01,<0.05). After treatment,the life quality,sleep condition,non nasal-eye symptoms,behavior issue of the combination group were superior to those of the moxibustion group(all<0.05),and at follow-up,the above outcomes,the eye and nasal symptoms as well as emotional response of the mo-xibustion group were better(all<0.05). There was statistical significance in the two group about serum IFN-,IgE,IL-4 before and after treatment(<0.01,<0.05),and significance existed between the two groups(<0.05).</p><p><b>CONCLUSIONS</b>Moxibustion combined with autohemotherapy of acupoint injection can effectively improve the short-term quality of life of AR withdeficiency of lung and spleen. The mechanism may be related to balancing the immune response of Th1 and Th2.</p>
