ABSTRACT
Modern immunotherapy has developed powerful tools for mounting antitumor response which nevertheless have had only limited success in clinic. Tumor cells use different mechanisms to escape from immune system. Thus, one of the reasons of unsuccessful immunotherapy might be induction of tolerance of tumor-specific cytotoxic lymphocytes by tumor cells. Previously we have demonstrated expression of HLA-E molecule by the cells of melanoma cell lines. In this paper we have studied HLA-E-dependent mechanism of melanoma cell escape from immune response.
Subject(s)
Antineoplastic Agents/pharmacology , HLA Antigens/metabolism , Histocompatibility Antigens Class I/metabolism , Interferon-gamma/pharmacology , Melanoma/drug therapy , Melanoma/immunology , T-Lymphocytes, Cytotoxic/immunology , Antineoplastic Agents/therapeutic use , Cell Line, Tumor , Flow Cytometry , Gene Expression Regulation, Neoplastic/drug effects , Gene Expression Regulation, Neoplastic/immunology , HLA Antigens/biosynthesis , Histocompatibility Antigens Class I/biosynthesis , Humans , Immunoblotting , Interferon-gamma/therapeutic use , Polymerase Chain Reaction , HLA-E AntigensSubject(s)
Lymphocytes/immunology , Neoplasms/immunology , Animals , Antigens, CD/immunology , Antigens, Differentiation/immunology , Antigens, Ly/immunology , Apoptosis Regulatory Proteins/immunology , CTLA-4 Antigen , Humans , Immunoglobulin G/immunology , Killer Cells, Natural/immunology , Lectins, C-Type/immunology , NK Cell Lectin-Like Receptor Subfamily D/immunology , Programmed Cell Death 1 Receptor , Receptors, Immunologic/immunology , Receptors, NK Cell Lectin-Like , Receptors, Natural Killer CellABSTRACT
Targeted genetic modification of embryonic stem cells (ESC) was used to obtain nondifferentiated cell clones containing the foreign genetic material in the genome. It was demonstrated that transgenic animals may be obtained by ESC injection in preimplantation embryos and subsequent transplantation of the embryos into a recipient female. Using this method, we constructed chimeric animals with a modified genome.
Subject(s)
Embryo, Mammalian/cytology , Mice, Transgenic/genetics , Stem Cells/cytology , Animals , Female , MiceABSTRACT
Various types of human blood cells were tested for expression of the Tag7/PGRP-SA and TagL/PGRP-L proteins, which belong to the family of proteins possessing the lysozyme-like peptidoglycan recognition protein (PGRP) domain. Expression regulation by several factors was demonstrated.
