[Theoretical and practical aspects of vitaminology in development of animal husbandry in Ukraine]. / Teoretychni ta praktychni aspekty vitaminologii u rozvytku tvarynnytstva v Ukraïni.

The paper deals with fundamental and applied aspects ofvitaminology development in Ukraine. Its foundations have been laid by A. V. Palladin and R. V. Chagovets, leading scientists of the country, and continued by their followers. Joint efforts of scientists in the field of veterinary, medicine, food branch will permit creating new up-to-date technologies for production of vitamin drugs of a new generation with the corresponding financial support of the state. That is rather necessary for health of the future generation of people.

[Effect of ammonium chloride on NDP(P)+-dependent glutamate dehydrogenase from bovine liver]. / Osoblyvosti vplyvu khlorydu amoniiu na NAD(P)+-zalezhnu hlutamatdehidrohenaz z pechinky velykoï rohatoï khudobi.

It was shown that NAD(P)(+)-dependent glutamate dehydrogenase from bovine liver (EC 1.4.1.3) was activated under the action of ammonium chloride in concentration which corresponded to the level of ammonium in normal blood (2-10 mM). Ammonium chloride increases the activity of glutamate dehydrogenase 1.8 times. Having enhanced the level of ammonium chloride to the amount which corresponds to the level of ammonium ions in blood during the pathology (10-25 mM), this substance inhibited the enzyme activity of glutamate dehydrogenase. Influence of 20 mM ammonium chloride on IR-spectra of NAD(P)(+)-dependent glutamate dehydrogenase in the deuterium oxide solution leads to the widening of the absorption line at 3300 cm-1 toward the higher energy. It is supposed that inhibition action of ammonium ions on the activity of glutamate dehydrogenase is connected with partial destruction of stereoregular hydrogen bonds in the enzyme molecule.

[Interrelation of fetal metabolism and late pregnancy in animals]. / Deiaki aspekti vzaiemozv'iazku obminu rechovin u vagitnykh tvarin ta ïkh plodiv

Parameters of were determined for acid-alkali state of blood, energy, nitrogen, protein and mineral metabolism in fetuses and animals at the last period of pregnancy. It was established that one can observe the state of compensated acidosis, intensification of glycolytic and inhibition ot aerobic processes in animals with late pregnancy; that is evidenced by changes in the content of glucose, the level and the value of correlation of the components of lactate-, malate- and glutamate dehydrogenase systems, activity of lactate dehydrogenase, alkali phosphatase, alanine- and aspartate aminotransferase as well as electrolyte composition of the organism tissues. Analogous changes in metabolism are observe in the fetus tissues in late antenatal period of development.

[Level and correlation of metabolites of NAD(P)+-dependent dehydrogenase systems in newborn calf tissues in acute diarrhea]. / Riven' ta spivvidnoshennia metabolitiv NAD(P)+-zalezhnykh dehidrohenazovykh system u tkanynakh novonarodzhenykh teliat za hostrykh rozladiv travlennia.

Substantial disorders of redox and energetic processes are observed in the newborn calf tissues which is evidenced by changes in glucose, lactate oxalo-acetate, malate, citrate, alpha-ketoglytarate and glutamate concentrations, as well as in activity of lactate dehydrogenase and gamma-glutamyltransferase, succinate dehydrogenase and alkaline phosphatase and correlations of (NAD(p)/NAD(P)H) in blood cytoplasm and liver and kidney mitochondria.