ABSTRACT
A possibility to detect adenoviral protein--hexon, using specific antibodies by surface plasmon resonance (SPR) was demonstrated. The hexon of the human adenovirus 2 (Ad2) binds to antibodies immobilized on the sensor surface treated by KNCS and protein A Staphylococcus aureus. The specificity of antihexon antibodies was demonstrated by indirect method of fluorescent antibodies (MFA) and cellular variant of the immunoassay (cELISA).
Subject(s)
Adenoviruses, Human/isolation & purification , Antibodies, Viral/immunology , Antigen-Antibody Complex/analysis , Antigens, Viral/immunology , Capsid Proteins/immunology , Adenoviruses, Human/immunology , Antigen-Antibody Complex/immunology , Humans , Sensitivity and Specificity , Surface Plasmon ResonanceABSTRACT
A level of autoantibodies (aAB) to nerve growth factor (NGF) was measured in blood serum of children from 4 groups: 1) schizophrenic patients; 2) children from the families, in which one of the parents suffered with schizophrenia (high risk groups of schizophrenia); 3) children with residual-organic damages of CNS; 4) control group. This index was also determined in their mothers. Significant elevation of a titer of aAB to NGF was observed in blood of children from groups 1 and 2 as well as in their mothers, as compared with 3 and 4 groups. Among the mothers of the children from 1 and 2 groups there were met women with different endogenous mental disorders, with the disorders of personality as well as mentally healthy women. An increase of a level of aAB to NGF was found in all the women from groups 1 and 2, independently of their mental status including mentally healthy women. Such results allow to consider elevated level of aAB to NGF as a risk factor of mental pathology.
Subject(s)
Autoantibodies/immunology , Nerve Growth Factors/blood , Nerve Growth Factors/immunology , Schizophrenia/immunology , Adolescent , Child , Child, Preschool , Female , Humans , Infant , Maternal Welfare , Personality Disorders/diagnosis , Pregnancy , Risk Factors , Schizophrenic PsychologyABSTRACT
In 58 patients after performance of plasty with fatty-cutaneous and fasciocutaneous grafts, the state of circulation in the flaps was studied. The level of ischemic disorders was defined. The medicamentous complexes contributing to improvement of circulation in the grafts are suggested.
Subject(s)
Burns/surgery , Surgical Flaps/methods , Surgical Flaps/physiology , Adolescent , Adult , Aged , Burns/physiopathology , Child , Child, Preschool , Humans , Microcirculation , Middle Aged , Regional Blood FlowABSTRACT
The authors treated 603 sufferers with acute cold damage. The principal reversibility of tissue damage in local cold traumas at the prereactive period is shown. The difference of therapeutical tactics in freezing, primary cryonecrosis, local cold trauma at the prereactive period, frostbite is noted. The necessity of individual immunotherapy of the sufferers is stressed. Criteria for estimation of the effectiveness of aid to such patients are suggested.
