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1.
Front Bioeng Biotechnol ; 11: 1308725, 2023.
Article in English | MEDLINE | ID: mdl-38169725

ABSTRACT

Introduction: Pregnanediol-3-glucuronide (PdG), as the main metabolite of progesterone in urine, plays a significant role in the prediction of ovulation, threatened abortion, and menstrual cycle maintenance. Methods: To achieve a rapid and sensitive assay, we have designed a competitive model-based time-resolved fluorescence microsphere-lateral flow immunochromatography (TRFM-LFIA) strip. Results: The optimized TRFM-LFIA strip exhibited a wonderful response to PdG over the range of 30-2,000 ng/mL, the corresponding limit of detection (LOD) was calculated as low as 8.39 ng/mL. More importantly, the TRFM-LFIA strip was innovatively used for the quantitative detection of PdG in urine sample, and excellent recovery results were also obtained, ranging from 97.39% to 112.64%. Discussion: The TRFMLFIA strip possessed robust sensitivity and selectivity in the determination of PdG, indicating the great potential of being powerful tools in the biomedical and diagnosis region.

2.
Front Bioeng Biotechnol ; 10: 894100, 2022.
Article in English | MEDLINE | ID: mdl-35757804

ABSTRACT

The diversity of bacteria and their ability to acquire drug resistance lead to many challenges in traditional antibacterial methods. Photothermal therapies that convert light energy into localized physical heat to kill target microorganisms do not induce resistance and provide an alternative for antibacterial treatment. However, many photothermal materials cannot specifically target bacteria, which can lead to thermal damage to normal tissues, thus seriously affecting their biological applications. Here, we designed and synthesized bacteria-affinitive photothermal carbon dots (BAPTCDs) targeting MurD ligase that catalyzes the synthesis of peptidoglycan (PG) in bacteria. BAPTCDs presented specific recognition ability and excellent photothermal properties. BAPTCDs can bind to bacteria very tightly due to their chiral structure and inhibit enzyme activity by competing with D-glutamic acid to bind to MurD ligases, thus inhibiting the synthesis of bacterial walls. It also improves the accuracy of bacteria treatment by laser irradiation. Through the synergy of biochemical and physical effects, the material offers outstanding antibacterial effects and potentially contributes to tackling the spread of antibiotic resistance and facilitation of antibiotic stewardship.

3.
Talanta ; 233: 122465, 2021 Oct 01.
Article in English | MEDLINE | ID: mdl-34215102

ABSTRACT

As an important bioactive component in plants, chlorogenic acid (CGA) has been widely studied for its potential role in human health. In this work, cyan fluorescent silicon quantum dots were successfully synthesized via a simple one-pot method for the rapid detection of CGA. The optimal excitation and emission wavelength of the obtained SiQDs was 350 nm and 470 nm, respectively. When the CGA was added, the maximum emission intensity of the SiQDs can be effectively quenched due to dynamic and static mixed quenching mechanisms. More significantly, there was a remarkable linear correlation between fluorescence quenching efficiency and a broad concentration of CGA solution range from 10 to 150 µmol/L with a limit of detection (LOD) of 0.43 µmol/L. Furthermore, the proposed SiQDs were successfully applied to analyze CGA in coffee beans and instant coffee after simple pretreatment with satisfactory results. Based on these, a high sensitivity and excellent selectivity fluorescent probe detection system was constructed, and it provides a valuable platform for the detection of CGA and has broad application prospects in the biological and pharmaceutical analysis field.


Subject(s)
Quantum Dots , Chlorogenic Acid , Fluorescent Dyes , Humans , Limit of Detection , Silicon
4.
Analyst ; 146(15): 4954-4963, 2021 Jul 26.
Article in English | MEDLINE | ID: mdl-34259240

ABSTRACT

Iron, nitrogen-co-doped carbon quantum dots (Fe,N-CDs) were prepared via a simple one-step hydrothermal method. The quantum yield of fluorescence reached about 27.6% and the blue-emissive Fe,N-CDs had a mean size of 3.76 nm. The as-prepared carbon quantum dots showed good solubility, a high quantum yield, good biocompatibility, low cytotoxicity, and high photostability. Interestingly, the as-prepared Fe,N-CDs exhibited good selectivity and sensitivity toward both hematin and ferric ions, and the limit of detection for hematin and ferric ions was calculated to be about 0.024 µM and 0.64 µM, respectively. At the same time, Fe,N-CDs were used for imaging HeLa cells and showed that most Fe,N-CDs were detained in the lysosome. Thus, this fluorescent probe has potential application in the quantitative detection of hematin or Fe3+ in a complex environment and for determining Fe3+ at the cellular level.


Subject(s)
Quantum Dots , Carbon , Fluorescent Dyes/toxicity , HeLa Cells , Hemin , Humans , Ions , Iron , Nitrogen , Quantum Dots/toxicity
5.
Colloids Surf B Biointerfaces ; 191: 110987, 2020 Jul.
Article in English | MEDLINE | ID: mdl-32325360

ABSTRACT

Assessment of microbial viability plays a key role in human health protection. Optical imaging based on fluorescent dyes is a simple and convenient way to assess microbial viability. However, it is still a challenge to obtain stable, nontoxic and low-cost dyes. Herein, we prepared a nitrogen and phosphorus co-doped carbon nanodots (N, P-CDs) via a one-step solvothermal method. The prepared CDs possess plenty of functional groups and exhibit high stability, good biocompatibility, excellent photoluminescent and low toxicity. Especially, the properties of high quantum yield (89.9%) and highly negative surface charge (-41.9 mV) make the prepared N, P-CDs ideal materials for microbial differentiation. Compared with commercial dyes, our CDs are more stable, cost less, which can rapidly distinguish dead microorganisms from living ones with higher specificity.


Subject(s)
Bacteria/growth & development , Fluorescent Dyes/chemistry , Fungi/growth & development , Microbial Viability , Nanostructures/chemistry , Quantum Dots , Bacteria/classification , Bacteria/isolation & purification , Carbon/chemistry , Fungi/classification , Fungi/isolation & purification , HeLa Cells , Humans , Nitrogen/chemistry , Optical Imaging , Phosphorus/chemistry
6.
J Mater Chem B ; 8(5): 919-927, 2020 02 07.
Article in English | MEDLINE | ID: mdl-31912848

ABSTRACT

Copper ions (Cu2+) and l-cysteine (l-Cys) in the human body always play critical roles in various physiological processes, while abnormal Cu2+ and l-Cys concentrations in the biological system lead to many diseases. In this manuscript, Si-doped carbon dots (Si-CDs) with near-infrared fluorescence were designed for the detection of Cu2+ and l-Cys through the fluorescence "on-off-on" mode. The carbon dots exhibited not only excellent optical merits including good stability against photobleaching and high chemical stability, but also superior biological compatibility. Interestingly, due to the abundant amino groups distributed on the surface of Si-CDs, they could bind to copper ions to form cupric amine complexes and then quench the fluorescence of Si-CDs due to an electron transfer process. In addition, upon the addition of l-Cys, the FL intensity of Si-CDs could be effectively recovered accompanied with complexation between Cu2+ and the functional groups in l-Cys, due to which Cu2+ was removed from the surface of Si-CDs. Notably, as far as we know, these are the first red-emitting carbon dots for copper ion and l-Cys assays in water samples and human plasma samples. Furthermore, this strategy was successfully applied to the determination of Cu2+ and l-Cys in living systems, demonstrating great practicability in biomedical applications.


Subject(s)
Carbon/chemistry , Copper/analysis , Cysteine/analysis , Optical Imaging , Quantum Dots/chemistry , Silicon/chemistry , A549 Cells , HeLa Cells , Humans , Materials Testing , Molecular Structure , Particle Size , Spectrometry, Fluorescence , Surface Properties
7.
RSC Adv ; 10(17): 10067-10075, 2020 Mar 06.
Article in English | MEDLINE | ID: mdl-35498619

ABSTRACT

In recent years, carbon dot (CD)-based fluorescent sensors for selective ions or small biomolecules have drawn great attention. In this work, highly fluorescent CDs (QY = 21%) were prepared from 2,3-diamino pyridine as the precursor through a facile solvothermal process. The CDs showed high stability and a green emission in aqueous, and the optimal emission wavelength of CDs is 508 nm under the excitation wavelength of 438 nm. Interestingly, a CDs-based nanoprobe was developed for a selective and sensitive fluorescence quenching response to NO2 - in water, and the quenching mechanism was investigated in the work. Besides, the recovery rates of NO2 - in the range of 98-103.5% were found to be acceptable, indicating that the proposed CDs could be act as potential candidates for determination of nitrite ions in real samples. Meanwhile, the nanoprobe was also successfully employed in a visualization biosensing platform for determination of NO2 - in living cells due to its eminent biocompatibility.

8.
ACS Appl Mater Interfaces ; 11(47): 44566-44572, 2019 Nov 27.
Article in English | MEDLINE | ID: mdl-31682396

ABSTRACT

It is well known that the calcium ion is essential for maintaining life activities in living organisms, and it is of great significance to detect the intracellular calcium concentration. For the detection of calcium ions, we developed a new type of fluorescent carbon dots (CDs), whose surface was modified by ethylenebis(oxyethylenenitrilo)tetraacetic acid (EGTA) through a secondary hydrothermal method. This is a simple and convenient chemical preparation method because all reactions are carried out in the same autoclave, and the final product is directly the EGTA-modified CDs. The CDs exhibit bright blue fluorescence, and as the calcium concentration increases, the fluorescence intensity drops sharply. The fluorescence quenching correlates with the concentration of calcium ions and has a good linearity in the range of 15-300 µM with a detection limit of 0.38 µM. The experimental results confirmed that the detection of calcium ions by CDs is a static fluorescence quenching process. Also, cytotoxicity test and cellular imaging experiments have shown that the CDs are nontoxic and biocompatible.

9.
ACS Appl Mater Interfaces ; 11(32): 28732-28739, 2019 Aug 14.
Article in English | MEDLINE | ID: mdl-31339033

ABSTRACT

Immunomagnetic micro/nanoparticles (IMNs) have been widely used to isolate rare circulating tumor cells (CTCs) from blood samples for early diagnosis of cancers. However, when entering into biofluids, IMNs nonspecifically adsorb biomolecules and the in situ formed biomolecule corona covers IMN surface ligands and weakens the targeting capabilities of IMNs. In this work, we demonstrated that by surface coating of IMNs with red blood cell (RBC)-derived vesicles, the obtained biomimetic particles (RBC-IMNs) basically adsorb no biomolecules and maintain the CTC targeting ability when exposed to plasma. Compared to IMNs, RBC-IMNs exhibited an excellent cell isolation efficiency in spiked blood samples, which was improved to 95.71% from 60.22%. Furthermore, by using RBC-IMNs, we successfully isolated CTCs in 28 out of 30 prostate cancer patient blood samples and further showed the robustness of RBC-IMNs in downstream cell sequencing. The work presented here provides a new insight into developing targeted nanomaterials for biological and medical applications.


Subject(s)
Biomimetic Materials , Cell Separation , Nanoparticles/chemistry , Neoplastic Cells, Circulating , Prostatic Neoplasms/blood , Biomimetic Materials/chemistry , Biomimetic Materials/pharmacology , HeLa Cells , Humans , MCF-7 Cells , Male , Neoplastic Cells, Circulating/metabolism , Neoplastic Cells, Circulating/pathology , PC-3 Cells , Prostatic Neoplasms/pathology
10.
Nanoscale ; 11(17): 8293-8303, 2019 Apr 25.
Article in English | MEDLINE | ID: mdl-30977474

ABSTRACT

Capturing circulating tumor cells (CTCs) from peripheral blood for subsequent analyses has shown potential in precision medicine for cancer patients. Broad as the prospect is, there are still some challenges that hamper its clinical applications. One of the challenges is to maintain the viability of the captured cells during the capturing and releasing processes. Herein, we have described a composite material that could encapsulate a magnetic Fe3O4 core in a MIL-100 shell (MMs), which could respond to pH changes and modify the anti-EpCAM antibody (anti-EpCAM-MMs) on the surface of MIL-100. After the anti-EpCAM-MMs captured the cells, there was no need for additional conditions but with the acidic environment during the cell culture process, MIL-100 could realize automatic degradation, leading to cell self-release. This self-release model could not only improve the cell viability, but could also reduce the steps of the release process and save human and material resources simultaneously. In addition, we combined clinical patients' case diagnosis with the DNA sequencing and next generation of RNA sequencing technologies in the hope of precision medicine for patients in the future.


Subject(s)
Carcinoma, Hepatocellular/pathology , Liver Neoplasms/pathology , Metal-Organic Frameworks/chemistry , Neoplastic Cells, Circulating/metabolism , Antibodies, Immobilized/chemistry , Antibodies, Immobilized/immunology , Carcinoma, Hepatocellular/genetics , Cell Line, Tumor , Cell Survival , Epithelial Cell Adhesion Molecule/immunology , Ferrosoferric Oxide/chemistry , Humans , Liver Neoplasms/genetics , Magnetite Nanoparticles/chemistry , Male , Middle Aged , Mutation , Neoplastic Cells, Circulating/pathology , Transcriptome , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism
11.
ACS Nano ; 13(3): 2849-2857, 2019 03 26.
Article in English | MEDLINE | ID: mdl-30803232

ABSTRACT

Although anti-PD-1 immunotherapy is widely used to treat melanoma, its efficacy still has to be improved. In this work, we present a therapeutic method that combines immunotherapy and starvation therapy to achieve better antitumor efficacy. We designed the CMSN-GOx method, in which mesoporous silica nanoparticles (MSN) are loaded with glucose oxidase (GOx) and then encapsulate the surfaces of cancer cell membranes to realize starvation therapy. By functionalizing the MSN's biomimetic surfaces, we can synthesize nanoparticles that can escape the host immune system and homologous target. These attributes enable the nanoparticles to have improved cancer targeting ability and enrichment in tumor tissues. Our synthetic CMSN-GOx complex can ablate tumors and induce dendritic cell maturity to stimulate an antitumor immune response. We performed an in vivo analysis of these nanoparticles and determined that our combined therapy CMSN-GOx plus PD-1 exhibits a better antitumor therapeutic effect than therapies using CMSN-GOx or PD-1 alone. Additionally, we used the positron emission tomography imaging to measuring the level of glucose metabolism in tumor tissues, for which we investigate the effect with the cancer therapy in vivo.


Subject(s)
Antineoplastic Agents/pharmacology , Cell Membrane/chemistry , Immunotherapy , Melanoma, Experimental/therapy , Nanoparticles/chemistry , Silicon Dioxide/chemistry , Animals , Cell Membrane/immunology , Glucose Oxidase/chemistry , Glucose Oxidase/immunology , Glucose Oxidase/metabolism , Melanoma, Experimental/immunology , Melanoma, Experimental/pathology , Mice , Particle Size , Porosity , Silicon Dioxide/immunology , Surface Properties , Tumor Cells, Cultured
12.
Nano Lett ; 19(4): 2215-2222, 2019 04 10.
Article in English | MEDLINE | ID: mdl-30543300

ABSTRACT

Zika virus (ZIKV) has emerged as a global health threat due to its unexpected causal link to devastating neurological disorders such as fetal microcephaly; however, to date, no approved vaccine or specific treatment is available for ZIKV infection. Here we develop a biomimetic nanodecoy (ND) that can trap ZIKV, divert ZIKV away from its intended targets, and inhibit ZIKV infection. The ND, which is composed of a gelatin nanoparticle core camouflaged by mosquito medium host cell membranes, effectively adsorbs ZIKV and inhibits ZIKV replication in ZIKV-susceptible cells. Using a mouse model, we demonstrate that NDs significantly attenuate the ZIKV-induced inflammatory responses and degenerative changes and thus improve the survival rate of ZIKV-challenged mice. Moreover, by trapping ZIKV, NDs successfully prevent ZIKV from passing through physiologic barriers into the fetal brain and thereby mitigate ZIKV-induced fetal microcephaly in pregnant mice. We anticipate that this study will provide new insights into the development of safe and effective protection against ZIKV and various other viruses that threaten public health.


Subject(s)
Microcephaly/prevention & control , Nanoparticles/administration & dosage , Zika Virus Infection/prevention & control , Zika Virus/drug effects , Animals , Biomimetics/methods , Cell Membrane/drug effects , Cell Membrane/virology , Culicidae/drug effects , Culicidae/virology , Disease Models, Animal , Female , Fetus , Gelatin/administration & dosage , Gelatin/chemistry , Humans , Mice , Microcephaly/pathology , Microcephaly/virology , Nanoparticles/chemistry , Pregnancy , Zika Virus/pathogenicity , Zika Virus Infection/pathology , Zika Virus Infection/virology
13.
Talanta ; 185: 1-6, 2018 Aug 01.
Article in English | MEDLINE | ID: mdl-29759174

ABSTRACT

A new cyanide probe has been prepared by one-step synthesis and evaluated by UV-vis and fluorescent method. This probe is combined by a fluorene part and a hemicyanine group through a conjugated linker, which is found to show rapid response, high selectivity and sensitivity for cyanide anions with significant dual colorimetric and fluorescent signal changes in aqueous solution. An intramolecular charge transfer (ICT) effect plays a key role in the CN- sensing properties, and the details of this mechanism are further supported by DFT and TD-DFT calculations. The theoretical study shows that the introduction of CN- twists the original plane structure and blocks the ICT process in the whole molecule, which brings about the absorption blue-shift and the fluorescence quenching.

14.
Nanoscale ; 10(13): 6014-6023, 2018 Mar 29.
Article in English | MEDLINE | ID: mdl-29542756

ABSTRACT

Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. However, currently available strategies for enriching CTCs, such as magnetic activated cell sorting (MACS), face serious problems with purity due to nonspecific interactions between beads and leukocytes in the process of capturing. In the present study, the tumor-targeting molecule folic acid (FA) and magnetic nanoparticles (MNPs) were coated on the surface of red blood cells (RBCs) by hydrophobic interaction and chemical conjugation, respectively. The resulting engineered RBCs rapidly adhered to CTCs and the obtained CTC-RBC conjugates were isolated in a magnetic field. After treatment with RBC lysis buffer and centrifugation, CTCs were released and captured. The duration of the entire process was less than three hours. Cell counting showed that the capture efficiency was above 90% and the purity of the obtained CTCs was higher than 75%. The performance of the proposed method exceeded that of MACS® beads (80% for capture efficiency and 20% for purity) under the same conditions. The obtained CTCs could be successfully re-cultured and proliferated in vitro. Our engineered RBCs have provided a novel method for enriching rare cells in the physiological environment.


Subject(s)
Erythrocytes/cytology , Folic Acid/chemistry , Magnetite Nanoparticles , Neoplastic Cells, Circulating , Cell Adhesion , Cell Line, Tumor , Cell Separation , Epithelial Cell Adhesion Molecule , Humans
15.
Nanotechnology ; 29(13): 134004, 2018 Apr 03.
Article in English | MEDLINE | ID: mdl-29334363

ABSTRACT

Nanotechnology possesses the potential to revolutionize the diagnosis and treatment of tumors. The ideal nanoparticles used for in vivo cancer therapy should have long blood circulation times and active cancer targeting. Additionally, they should be harmless and invisible to the immune system. Here, we developed a biomimetic nanoplatform with the above properties for cancer therapy. Macrophage membranes were reconstructed into vesicles and then coated onto magnetic iron oxide nanoparticles (Fe3O4 NPs). Inherited from the Fe3O4 core and the macrophage membrane shell, the resulting Fe3O4@MM NPs exhibited good biocompatibility, immune evasion, cancer targeting and light-to-heat conversion capabilities. Due to the favorable in vitro and in vivo properties, biomimetic Fe3O4@MM NPs were further used for highly effective photothermal therapy of breast cancer in nude mice. Surface modification of synthetic nanomaterials with biomimetic cell membranes exemplifies a novel strategy for designing an ideal nanoplatform for translational medicine.


Subject(s)
Breast Neoplasms/therapy , Hyperthermia, Induced/methods , Low-Level Light Therapy/methods , Magnetite Nanoparticles/therapeutic use , Molecular Targeted Therapy/methods , Theranostic Nanomedicine/methods , Animals , Biological Transport , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Cell Membrane/immunology , Cell Membrane/metabolism , Female , Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/metabolism , Humans , Immune Evasion , MCF-7 Cells , Magnetite Nanoparticles/ultrastructure , Mice , Mice, Inbred BALB C , Mice, Inbred ICR , Mice, Nude , RAW 264.7 Cells , Xenograft Model Antitumor Assays
16.
Nanotechnology ; 29(8): 084002, 2018 02 23.
Article in English | MEDLINE | ID: mdl-29339567

ABSTRACT

Recently, red blood cell (RBC) membrane-coated nanoparticles have attracted much attention because of their excellent immune escapability; meanwhile, gold nanocages (AuNs) have been extensively used for cancer therapy due to their photothermal effect and drug delivery capability. The combination of the RBC membrane coating and AuNs may provide an effective approach for targeted cancer therapy. However, few reports have shown the utilization of combining these two technologies. Here, we design erythrocyte membrane-coated gold nanocages for targeted photothermal and chemical cancer therapy. First, anti-EpCam antibodies were used to modify the RBC membranes to target 4T1 cancer cells. Second, the antitumor drug paclitaxel (PTX) was encapsulated into AuNs. Then, the AuNs were coated with the modified RBC membranes. These new nanoparticles were termed EpCam-RPAuNs. We characterized the capability of the EpCam-RPAuNs for selective tumor targeting via exposure to near-infrared irradiation. The experimental results demonstrate that EpCam-RPAuNs can effectively generate hyperthermia and precisely deliver the antitumor drug PTX to targeted cells. We also validated the biocompatibility of the EpCam-RAuNs in vitro. By combining the molecularly modified targeting RBC membrane and AuNs, our approach provides a new way to design biomimetic nanoparticles to enhance the surface functionality of nanoparticles. We believe that EpCam-RPAuNs can be potentially applied for cancer diagnoses and therapies.

17.
J Mater Chem B ; 6(21): 3549-3554, 2018 Jun 07.
Article in English | MEDLINE | ID: mdl-32254450

ABSTRACT

Carbon nanodots (CDs) are novel forms of zero-dimensional carbonaceous nanomaterials, which have attracted the attention of researchers. Long wavelength emission decreases the interference of auto-fluorescence of tissue and can also penetrate more deeply. However, it is still a challenge to develop red emissive CDs. In this work, nitrogen, sulfur co-doped CDs are synthesized with red light emission through one-step solvothermal treatment. The as-synthesized CDs exhibit excellent properties such as high chemical stability, good biocompatibility, excellent photoluminescence properties, good water solubility, and low toxicity, and also show high sensitivity and selectivity towards Fe3+ sensing. The detection limit is as low as 17.3 nM. In addition, the CDs are successfully utilized for monitoring Fe3+ in living cells, which demonstrates their promising applicability in bioimaging. Furthermore, a test paper based on the CQDs has been prepared. This developed nanosensor can detect Fe3+ visually and sensitively without any further steps which will make a great contribution to the development of ion sensors.

18.
J Phys Chem A ; 122(1): 217-223, 2018 Jan 11.
Article in English | MEDLINE | ID: mdl-29232518

ABSTRACT

The development of probes for rapid and selective detection of peroxynitrite in vivo is of great importance in biological science. We investigate different photoinduced electron transfer (PIET) processes of two generations of peroxynitrite probes. Each has fluorescein and phenol moieties; one is conjugated by an ether linkage while the other is conjugated via an amine linkage. Using theoretical calculations, we demonstrated that the PIET in the probe with an ether linkage occurs from the benzoic acid to the xanthene moiety. In contrast, the PIET in the probe with an amine linkage occurs from the phenol moiety to the fluorescein. This suggests that better sensitivity can be accomplished in probes with an amine linkage than with an ether linkage. Following this model, we designed two novel peroxynitrite probes and simulated their detection capabilities in the near-infrared region.

19.
Biomater Sci ; 3(7): 1147-56, 2015 Jul.
Article in English | MEDLINE | ID: mdl-26221947

ABSTRACT

Near-infrared (NIR) light-triggered drug release systems are promising for drug delivery applications in view of the advantages of NIR light, which include high tissue penetration and low damage. In this report, we developed nanogels (NGs) by supramolecular self-assembly from adamantine (AD)-conjugated copolymer, poly[poly(ethylene glycol)monomethyl ether metharcylate]-co-poly(N-(2-hydroxypropyl)methacrylamide)-co-poly(N-adamantan-1-yl-2-methacrylamide) (PPEGMA-co-PHPMA-co-PADMA), and ß-cyclodextrin (ß-CD)-functionalized poly(amidoamine) (PAMAM) dendrimer based on the host-guest interaction of the AD and ß-CD moieties, and they were used to encapsulate indocyanine green (ICG) and doxorubicin (DOX) for combined photothermal-chemotherapy. NGs simultaneously loading ICG and DOX (DINGs) showed significant photothermal effects and stimuli-triggered drug release under NIR laser irradiation by the photothermal-induced relaxation or dissociation of the NGs. In vitro cytotoxicity evaluation of DINGs under NIR irradiation demonstrated the synergistic effects of hyperthermia, photothermal-triggered drug release, and chemotherapy. In vivo investigation revealed their high accumulation in tumor tissue and significant tumor growth suppression under NIR irradiation. These NIR light-triggered drug release NGs represent efficient and promising anticancer drug vectors for the combined photothermal-chemotherapy of cancer to maximize therapeutic efficacy and minimize side effects.


Subject(s)
Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacokinetics , Doxorubicin/chemistry , Infrared Rays/therapeutic use , Polyethylene Glycols/chemistry , Polyethyleneimine/chemistry , Polymethacrylic Acids/chemistry , beta-Cyclodextrins/chemistry , Antineoplastic Agents/metabolism , Chemistry, Pharmaceutical , Cycloaddition Reaction , Doxorubicin/pharmacology , Drug Screening Assays, Antitumor , Humans , Micelles , Nanogels , Particle Size , Photochemical Processes , Temperature
20.
Chem Commun (Camb) ; 50(58): 7824-7, 2014 Jul 25.
Article in English | MEDLINE | ID: mdl-24909859

ABSTRACT

The polymeric nanogels were constructed via host-guest interactions for dual pH-triggered multistage drug delivery, which showed tumor acidity-triggered nanogel reorganization into smaller nanoparticles for deep tissue penetration, high-efficiency cellular uptake, and intracellular endo-lysosomal pH-responsive drug release.


Subject(s)
Drug Delivery Systems/methods , Polyethylene Glycols/chemistry , Polyethyleneimine/chemistry , Polymers/chemistry , Doxorubicin/administration & dosage , Hydrogen-Ion Concentration , Magnetic Resonance Spectroscopy , Nanogels , beta-Cyclodextrins/chemistry
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