ABSTRACT
OBJECTIVE: Various microRNAs (miRNAs) have been reported to be involved in the pathogenesis and development of human cancers, including papillary thyroid carcinoma (PTC). However, the role of miR-224-5p in PTC progression remains unclear. Therefore, the purpose of this study is to illuminate the function of miR-224-5p in PTC. PATIENTS AND METHODS: Expression of miR-224-5p and EGR2 was examined in PTC by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Transwell assay was used to detect cell migration and invasion. Western blot analysis was used to detect epithelial-mesenchymal transition (EMT). The relationship between miR-224-5p and EGR2 was confirmed by Dual-Luciferase assay. RESULTS: Upregulation of miR-224-5p and downregulation of EGR2 expression were detected in PTC tissues and cells. Upregulation of miR-224-5p was found to be associated with TNM stage and lymph node metastasis. Meanwhile, it also predicted poor prognosis in PTC patients. Functionally, upregulation of miR-224-5p promoted cell metastasis and EMT in PTC. In addition, miR-224-5p was detected to directly target EGR2. EGR2 expression was negatively correlated with EGR2 expression in PTC. Of note, overexpression of EGR2 attenuated the carcinogenic effects of miR-224-5p in PTC. CONCLUSIONS: MiR-224-5p promotes cell migration, invasion, and EMT in PTC by targeting EGR2.
Subject(s)
Carcinoma, Papillary/metabolism , Early Growth Response Protein 2/metabolism , MicroRNAs/metabolism , Thyroid Neoplasms/metabolism , Carcinoma, Papillary/diagnosis , Cells, Cultured , Early Growth Response Protein 2/genetics , Female , Humans , Male , MicroRNAs/genetics , Middle Aged , Thyroid Neoplasms/diagnosisSubject(s)
Ear Canal/parasitology , Mite Infestations/drug therapy , Mite Infestations/parasitology , Pyroglyphidae/drug effects , Administration, Topical , Animals , Antiparasitic Agents/administration & dosage , Boric Acids/administration & dosage , Hydrogen Peroxide/administration & dosage , Instillation, Drug , Mite Infestations/diagnosisABSTRACT
AIM: To investigate the significance and changes of Aquaporin 2 (AQP2) in the rat renal graft of acute rejection (AR). METHODS: Wistar recipients of Spraque-Dawley or Wistar renal grafts were treated with cyclosporine (CsA). Renal grafts were harvested at various times after transplantation for analysis of the levels of AQP2 mRNA and protein of by RT-PCR and immunohistochemistry. RESULTS: The expression of AQP2 mRNA and protein in the acutely rejecting were grafts significantly decreased (P < 0.05) compared with the control group. But the expression of AQP2 mRNA and protein in the syngeneic grafts (sTX) versus the immunosuppression group (aTX+CsA) showed no difference compared with a control group (P > 0.05). Furthermore, at day 5 and day 7 after transplantation the expressions of AQP2 expression in the allogeneic group (aTX) were decreased significantly compared with day 3 after transplantation (P < 0.05). In addition, there was no remarkable difference at day 5 or 7 after transplantation. CONCLUSION: AQP2 mRNA and protein expressions were down-regulated during renal transplant acute rejection, which had no relationship to the ischemia reperfusion injury and denervation damage. Furthermore, CsA administration after kidney transplantation blunted this down-regulation (P > 0.05).
Subject(s)
Aquaporin 2/genetics , Graft Rejection/pathology , Kidney Transplantation/pathology , Animals , Aquaporin 2/metabolism , Cyclosporine/pharmacology , DNA Primers , Gene Expression Regulation/physiology , Graft Rejection/immunology , Graft Rejection/metabolism , Immunohistochemistry , Immunosuppression Therapy , Immunosuppressive Agents/pharmacology , Kidney Transplantation/immunology , Male , RNA, Messenger/genetics , Rats , Rats, Sprague-Dawley , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Transplantation, Homologous/pathology , Transplantation, Homologous/physiology , Transplantation, Isogeneic/pathology , Transplantation, Isogeneic/physiologyABSTRACT
AIM: The purpose of this study was to provide practical anatomic data for imaging diagnosis of olfactory pathways and operation of nasal cavity and anterior cranial fossae. METHODS: Sectional anatomy of olfactory pathways were investigated specially on 17 sets of Chinese adult cadavers and 9 sets of serial magnetic resonance (MR) imaging of normal adult on serial transverse, sagittal and coronal sections respectively. RESULTS: We recognized olfactory bulb, olfactory tract, medial and lateral olfactory striae, olfactory trigone, anterior perforated substance and piriform lobe on transverse, sagittal and coronal sections respectively. On the 5-7 serial coronal sections from crista galli of ethmoid bone to the optic chiasm, the cusp ellipse olfactory bulb and the triangular tract were situated in the shallow part of the olfactory sulcus. CONCLUSION: The olfactory bulb and olfactory tract lay tightly on the ethmoidal cribriform plate and jugum sphenoidale, in the olfactory cistern of the shallow part of the olfactory sulcus, the ethmoid sinus and sphenoid sinus inferiorly.
