ABSTRACT
The multi-kinase inhibitor sorafenib is a primary treatment modality for advanced-stage hepatocellular carcinoma (HCC). However, the therapeutic benefits are short-lived due to innate and acquired resistance. Here, we examined how HCC cells respond to sorafenib and adapt to continuous and prolonged exposure to the drug. Sorafenib-adapted HCC cells show a profound reprogramming of mitochondria function and marked activation of genes required for mitochondrial protein translation and biogenesis. Mitochondrial ribosomal proteins and components of translation and import machinery are increased in sorafenib-resistant cells and sorafenib-refractory HCC patients show similar alterations. Sorafenib-adapted cells also exhibited increased serine 727 phosphorylated (pSer727) STAT3, the prevalent form in mitochondria, suggesting that STAT3 might be an actionable target to counteract resistance. Consistently, a small-molecule STAT3 inhibitor reduces pSer727, reverts mitochondrial alterations, and enhances the response to sorafenib in resistant cells. These results sustain the importance of mitochondria plasticity in response to sorafenib and identify a clinically actionable strategy for improving the treatment efficacy in HCC patients.
ABSTRACT
La Oficina Intercultural Willaqkuna surgió como respuesta a los problemas de calidad percibida de la población, particularmente de los grupos originarios y rurales.
Subject(s)
Culture , Hospitals , Medicine, Traditional , Health Facility Merger , Indigenous PeoplesABSTRACT
La presencia de 45 millones de indígenas pertenecientes a más de 400 pueblos originarios diferentes, patentiza la diversidad cultural de la población en el continente americano.