Evidence mapping of current status, impact, prevention and control measures from rabies research in Bangladesh (2010-2021): a scoping review protocol.

INTRODUCTION: Rabies is one of the priority zoonotic diseases in Bangladesh. Though the rabies cases have been reduced over the years due to the mass dog vaccination programme since 2011 throughout the country, it is still a major health problem in Bangladesh with an annual estimated 200 000 animal bite cases and over 2000 human deaths. This article presented a scoping review protocol for published literature on rabies in Bangladesh and believes to create impact in Bangladesh by identifying the research gap and guiding the evidence-informed policy adaptation from its findings in the future which will strongly underscore the elimination of Rabies and reduce preventable mortalities. We will attempt to synthesise evidence descriptively on burden and impact of rabies in human population as well as the awareness level and effective control and preventive measures from the available studies on rabies from 2010 to 2021 in Bangladesh. METHODS: The scoping review is planned following the Joanna Briggs Institute methodology and the major guiding steps are: defining the research questions, determining the eligibility criteria with population, concept and context strategy, stating the plan for evidence searching, selection and data collection, searching for evidence and selection of literature by the inclusion criteria, data collection, descriptive analysis and presentation of the data and reporting of the findings. Search will be conducted for both published and grey literature in English language. Blinded screening processes will be adapted to prevent bias among reviewers. ETHICS AND DISSEMINATION: A scoping review synthesises existing knowledge and does not necessitate ethical approval. Results of this scoping review will be submitted to a journal for publication, presented in relevant conferences and disseminated on social media platforms (eg, Twitter) among the global health stakeholders.

Isolation and antimicrobial resistance of motile Salmonella enterica from the poultry hatchery environment.

Salmonella is a globally distributed major food-borne pathogen and poultry is one of the predominant sources of salmonellosis in humans. To investigate the presence of motile Salmonella in the poultry hatchery environment, we collected 97 fluff samples from four selected broiler breeder chicken hatcheries from Chattogram, Bangladesh during July-December 2015. To isolate motile Salmonella enterica, we used conventional bacteriological techniques followed by serological verification using anti-Salmonella Poly A-E serum and species confirmation by conventional PCR assay. Antimicrobial susceptibility testing by Kirby-Bauer disc diffusion method for 10 commonly used antibiotics was performed on all isolates. Isolates displaying phenotypic resistance to ampicillin were tested by PCR for blaTEM gene, whereas those resistant to tetracycline were tested for the presence of tetA, tetB and tetC genes. A total of 24 samples (24.7%; 95% CI: 16.5-34.5, N = 97) from 3 hatcheries were positive for motile Salmonella. Of them, 21 (87.5%) and 12 (50.0%) were resistant to ampicillin and tetracycline, respectively, 9 (37.5%) to nalidixic acid and sulphamethoxazole/trimethoprim. No resistance was detected to ceftriaxone, cefoxitin, gentamicin, neomycin, ciprofloxacin and colistin. Ten (42%) of 24 isolates from 2 hatcheries were multi-drug resistant (i.e. resistant to ≥ 3 antimicrobial classes). Six of 21 ampicillin resistant isolates contained blaTEM gene and 10 of 12 tetracycline resistant isolates contained tetA gene. This study highlights the circulation of multi-drug resistant motile Salmonella in the hatchery environment for the first time in Bangladesh. Further epidemiological and molecular studies are therefore needed to identify the serotypes and source of the bacteria in hatcheries.