Synergistic effect of Zataria Multiflora essential oil on doxorubicin-induced growth inhibition of PC3 cancer cells and apoptosis.

Today, herbs are used as adjuncts to reduce the toxicity of chemotherapy drugs. Here, Zataria-Multiflora Essential Oil (ZEO) was concomitantly employed with doxorubicin, as an anti-cancer drug, to reduce the doxorubicin dosage. The growth inhibition was determined using MTT assay in treated cells. The morphological alteration was observed by fluorescent staining. To verify and compare the apoptosis, AnnexinV-PI flowcytometry and DNA fragmentation assay were performed, and the influence of the compounds on ROS generation was assessed. Changes in MMP and protein expression were analyzed by flowcytometry and western blot, respectively. The results showed that ZEO can act as an amplifier to sensitize PC3 prostate cancer cells to undergo ROS generation and apoptosis. This amplification can heighten the doxorubicin efficacy in lower doses. Consequently, our results indicated that doxorubicin-ZEO combinatory treatment of PC3 cells can reduce the nonspecific toxicity of doxorubicin and can be considered as a candidate in combinatory therapy.

The Effect of Need-oriented Educational Intervention on the General Health of the Elderly.

INTRODUCTION: Improving the health of the elderly must be based on their special needs that can be achieved through self-needs assessments through education, which is one of the most important tools in the field of health improvement. The aim of the present study was to evaluate the effect of need-oriented educational interventions on the general health of the elderly living in nursing homes. MATERIALS AND METHODS: The present study is a clinical trial conducted among the elderly living at the Sadeghieh nursing home of Isfahan, Iran. Seventy elderly people who satisfied the inclusion criteria were selected and divided into two groups of case and control. The case group went through 8 sessions of group therapy for educating participants who were previously investigated through needs assessment. The control group received the usual health care. The general health level of the participants was evaluated before and 1 week and 1 month after the intervention using Goldberg's general health questionnaire. Data were analyzed using Chi-square test, independent t-test, paired t-test, Mann-Whitney test, and variance analysis. RESULTS: The score of general health had no significant difference between both the groups before the intervention, however, the difference in the score between both the groups 1 week and 1 month after the intervention was significant (1 week after the intervention, the scores were 15.6 and 30.3, and 1 month after the intervention, the scores were 16 and 32.2, respectively) (P < 0.001). CONCLUSIONS: Need-oriented educational intervention is an effective, safe, and inexpensive method for improving the general health of the elderly living in nursing homes.

The impact of group training about parenting styles on maternal attitudes toward parenting styles.

BACKGROUND: Parenting style is one of the most important and effective factors in training and growth of children and adolescents and the method that parents communicate with their children is an effective factor on family contact models. OBJECTIVES: The aim of this study was to determine the impact of group training about parenting styles on maternal attitudes that were admitted to Isfahan Imam Ali (AS) health care center in 2013. MATERIALS AND METHODS: This was an experimental study, which was conducted on a random sample of 25 mothers referred to this health care center. They were divided into two groups (experimental and control). The experimental group received five sessions of group training, and the control group received a booklet about parenting styles. The used tool in this study was the Bamerind Parenting Style Questionnaire that was completed by the mothers before and after the intervention and finally, their obtained scores were compared with each other. RESULTS: The results of the present study showed that the mean score of attitude toward easy-going style in test group was less than the control group after intervention (P = 0.045). The mean score of attitude toward authoritative style in the experimental group was less than control group after intervention (P = 0.037) and the mean score of attitude toward authoritative style in the experimental group was more than the control group after intervention (P = 0.011). CONCLUSIONS: Group training can be an appropriate method in changing maternal attitudes toward parenting styles.