ABSTRACT
Results of investigation of the chicken and mouse interferons by electrofocussing in sucrose and in polyacrylamide gel are described. The isoelectric points of these interferons were determined. The advantages of the electrofocussing in gel over that in sucrose for the interferon analysis was shown.
Subject(s)
Interferons/isolation & purification , Animals , Chickens , Electrophoresis, Polyacrylamide Gel , Isoelectric Focusing , Mice , Species Specificity , SucroseSubject(s)
Antigens, Viral , Lymphoma, Non-Hodgkin/microbiology , Retroviridae/immunology , Viral Proteins/analysis , AKR murine leukemia virus/immunology , Animals , Electrophoresis, Polyacrylamide Gel , Epitopes , Mice , Mice, Inbred Strains , Peptides/analysis , Rauscher Virus/immunology , Retroviridae/analysis , Sarcoma, ExperimentalABSTRACT
The properties of murine oncornavirus produced by cells of spontaneous lymphosarcroma of CC57Br mice are described. In addition to the properties common for C-type RNA tumor viruses such as 60-70 S high molecular weight RNA, the presence of RNA-directed DNA polymerase and murine gs I (intraspecies) antigen and typical morphology in electron microscope, etc., the virus under study is characterized by instability of virions, unusual features of the DNA polymerase system and by the absence of demonstrable oncogenicity either in laboratory animals or in tissue cultures.
