ABSTRACT
AIM: Detection of features of qualitative and quantitative composition of large intestine microbiocenosis of humans with various degree of local metabolic disorders during dysbacterioses due to various causes. MATERIALS AND METHODS: Microflora of large intestine and content of malonic dialdehyde (MDA) in coprofiltrates of 330 adult humans with large intestine dysbacterioses due to various causes were studied. RESULTS: It was established, that high MDA content in coprofiltrates matches higher quantities of opportunistic microorganisms and atypical escherichia in microflora composition of large intestine. Relation of MDA composition in coprofiltrates and factors that cause dysbacteriosis were not detected. CONCLUSION: The studies performed give evidence that changes in local metabolic activity may be a single mechanism of development of large intestine dysbacterioses that are caused by various factors.
Subject(s)
Biomarkers/analysis , Candida/isolation & purification , Gastroenteritis/microbiology , Gram-Negative Bacteria/isolation & purification , Gram-Positive Bacteria/isolation & purification , Intestine, Large/microbiology , Malondialdehyde/analysis , Adolescent , Adult , Aged , Anti-Bacterial Agents/adverse effects , Candida/drug effects , Candida/genetics , Colony Count, Microbial , Feces/chemistry , Female , Gastroenteritis/drug therapy , Gram-Negative Bacteria/drug effects , Gram-Negative Bacteria/genetics , Gram-Positive Bacteria/drug effects , Gram-Positive Bacteria/genetics , Humans , Male , Microbial Consortia , Middle Aged , Severity of Illness IndexSubject(s)
Gastroenteritis/diagnosis , Hemagglutination Tests/methods , Rotavirus Infections/diagnosis , Animals , Antibodies, Viral/analysis , Ascitic Fluid/immunology , Child, Preschool , Erythrocytes/immunology , Feces/microbiology , Humans , Infant , Rats , Rotavirus/growth & development , Rotavirus/immunology , Rotavirus/isolation & purification , Virus Cultivation/methodsABSTRACT
The possibility of using indirect hemagglutination test (IHA) with erythrocyte immunoglobulin diagnosticum for detection of CHF virus in the blood of patients early after the onset was studied. It was shown that viremia could be detected by the IHA test within 2-3 hours provided the blood was collected not later that 8 days after the onset of the disease. The sensitivity of the test for the detection of viremia is as high as that of bioassays. The IHA test may also be used for the detection of CHF virus specific antigen in cadaver materials (blood, organ suspensions) even in those cases when virus isolation is prevented by toxicity of the specimens.
