ABSTRACT
The effect of cultured autologous oral keratinocyte suspension in fibrin glue on the healing of surgically produced oral mucosal wounds was assessed in the rabbit model. Using the light microscope and a digital image analysis system, the epithelization parameters (marginal epithelization and percentage of wound re-epithelization) were measured in haematoxylin-eosin stained sections of the wound area and compared with those of wounds treated with fibrin glue alone and untreated ones. The epithelization was significantly higher in keratinocytes plus fibrin glue-treated wounds on postoperative days 3 and 7. No significant differences were observed on postoperative day 1, when the healing process had just begun, and on postoperative day 14, when re-epithelization was completed or nearly completed in all groups. The inflammatory infiltration of the wounded mucosa was weakest in keratinocyte-treated wounds and strongest in untreated wounds. In conclusion, suspension of cultured autologous oral keratinocytes in fibrin glue significantly accelerates oral wound healing in the rabbit model and could be beneficial in the treatment of oral wounds in patients.
Subject(s)
Biological Dressings , Fibrin Tissue Adhesive/administration & dosage , Keratinocytes/transplantation , Mouth Mucosa/drug effects , Wound Healing/physiology , Animals , Cells, Cultured , Combined Modality Therapy , Keratinocytes/cytology , Mouth Mucosa/surgery , Rabbits , Transplantation, Autologous , Wound Healing/drug effectsABSTRACT
AIM: A pilot study to compare pain perception during caries treatment in children by means of: the Vector system versus a mechanical method. STUDY DESIGN AND METHODS: A population of 31 children, aged 7-11 years, with a positive attitude towards dental treatment was recruited. Every child had two permanent molar teeth treated, one using the Vector system (piezo-driven ultrasonic device) and the other using conventional method (dental bur). Corah, Hochman and the visual scale (Facial Expression Scale) were applied to evaluate anxiety and pain perception. RESULTS: Treatment with the Vector system required significantly longer time 31.1 versus 4.7 mins for the conventional method. With the Vector system 54.8% of children and with conventional method 29.0% felt no pain. Girls admitted to feeling more pain than boys (verbal scale p < 0.018, visual scale p < 0.072). A high statistical correlation between verbal and visual scales was confirmed (r = 0.821; p < 0.001 and a greater level of pain was found using conventional method. CONCLUSIONS: The Vector system is useful in treating caries in children, because it minimizes the negative attitudes to pain but takes significantly longer to use.
Subject(s)
Dental Caries/therapy , Dental Cavity Preparation/methods , Pain/physiopathology , Air Abrasion, Dental , Child , Dental Anxiety/psychology , Dental Cavity Preparation/instrumentation , Female , Humans , Male , Molar/pathology , Pain Measurement , Pain Threshold/physiology , Pilot Projects , Sex Factors , Time Factors , Ultrasonic TherapyABSTRACT
The reported study was carried out on 1241 subjects aged from 5 to 25 years with symptoms of parodontal diseases who came to an outpatient clinic in the years 1969-1988. Gingivitis prevailed among the diagnoses--825 cases, while deep parodontal changes were found in 416 cases. The incidence of all parodontal diseases was higher in females, with the exception of ulcerative gingivitis which was more frequent in males. The patients from the City of Cracow prevailed over those from the provinces of Southern Poland, and the incidence of various disease entities varied in various years.
