1.
Science
; 292(5524): 2073-5, 2001 Jun 15.
Article
in English
| MEDLINE
| ID: mdl-11408656
ABSTRACT
Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically derived energy. We show that the microalga Phaeodactylum tricornutum can be genetically engineered to thrive on exogenous glucose in the absence of light through the introduction of a gene encoding a glucose transporter (glut1 or hup1). This demonstrates that a fundamental change in the metabolism of an organism can be accomplished through the introduction of a single gene. This also represents progress toward the use of fermentation technology for large-scale commercial exploitation of algae by reducing limitations associated with light-dependent growth.
Subject(s)
Diatoms/genetics , Diatoms/metabolism , Genetic Engineering , Glucose/metabolism , Monosaccharide Transport Proteins/genetics , Biological Transport , Cell Line, Transformed , Cell Membrane/metabolism , Darkness , Diatoms/growth & development , Glucose Transporter Type 1 , Glycolysis , Green Fluorescent Proteins , Humans , Light , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Membrane Proteins/genetics , Membrane Proteins/metabolism , Microscopy, Confocal , Molecular Weight , Monosaccharide Transport Proteins/chemistry , Monosaccharide Transport Proteins/metabolism , Photosynthesis , Recombinant Fusion Proteins/metabolism , Symporters , Transformation, Genetic
2.
Vestn Oftalmol
; 106(4): 75, 1990.
Article
in Russian
| MEDLINE
| ID: mdl-2238336
3.
Vestn Oftalmol
; (2): 3-4, 1980.
Article
in Russian
| MEDLINE
| ID: mdl-6446185
4.
Sov Zdravookhr
; (10): 26-8, 1977.
Article
in Russian
| MEDLINE
| ID: mdl-145001
5.
Vestn Oftalmol
; : 75-6, 1969.
Article
in Russian
| MEDLINE
| ID: mdl-5771469