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Ukr Biokhim Zh (1978) ; 51(4): 374-7, 1979.
Article in Ukrainian | MEDLINE | ID: mdl-224542

ABSTRACT

The article deals with the process of immobilizing the enzymic preparation amylosubtilin G10x by the carriers activated with 2,4-toluylenediisocyanate and cyanurchloride which were obtained on the basis of silanized sylochrome. The 2,4-toluylenediisocyanate activated carrier is shown to be high-effective in immobilization. The activity of the preparations immobilized by the carrier activated with 2,4-toluylenediisocyatate is 75 units per 1 g of carrier; for the preparation immobilized on the carrier activated with cyanurchloride it is equal to 2.9 u/g. The specific activity is 3.1 and 0.9 u/mg, respectively. Immobilization on the tested carriers decreases the enzymic preparation thermostability.


Subject(s)
Amylases/metabolism , Enzymes, Immobilized/metabolism , Drug Stability , Kinetics , Methods , Temperature , Toluene 2,4-Diisocyanate , Triazines
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