Subject(s)
Female , Humans , Adolescent , Anodontia/classification , Anodontia/etiology , Anodontia/genetics , Genetic Diseases, X-Linked/classification , Genetic Diseases, X-Linked/genetics , Genetic Diseases, X-Linked/pathology , Anodontia/diagnostic imaging , Comprehensive Dental Care/methods , Diagnosis, Differential , Dental Enamel Hypoplasia/etiology , Ectodermal Dysplasia/diagnosis , Papillon-Lefevre Disease/diagnosisABSTRACT
Los mastocitos son células del tejido conectivo ampliamente distribuidas en la mucosa digestiva y respiratoria, especialmente cerca de sitios de respuesta inmune. El presente estudio se efectuó con el propósito de evaluar la distribución de los mastocitos en las glándulas salivales mayores (parótida, submaxilar y sublingual) de rata. Las muestras de tejido glandular fueron incluidas en parafina y los cortes histológicos obtenidos se colorearon con Azul alcian-safranina y Azul de Toluidina. Posteriormente se efectuó la cuantificación de mastocitos/mm2 considerando dos áreas glandulares: el estroma intralobulillar y el interlobulillar. Los resultados no muestran variaciones significativas en la población de mastocitos al comparar las tres glándulas (p>0,05), pero si se encontró una mayor presencia de mastocitos en relación con la vía excretora principal. Los resultados en conjunto sugieren una activa participación de los mastocitos en los mecanismos de detección de antígenos que ingresan a las glándulas salivales y su estrecha relación con otras células captadoras de antígenos como las células dendríticas.
Mast cells (MT) are connective tissue cells widely distributed throughout the body, especially in immune mucosal response sites like the digestive and air way tract. The aim of the present study was to find the number and the pattern of distribution and possible differences in mast cell population present in the mayor salivary glands (parotid, submandibular and sublingual glands) of rats. Fragments from salivary glands were collected, processed an included in paraffin wax, cut and stained with alcian blue-safranin and toluidine blue. The total number of MT was counted to estimate the mm² population from both intralobulillar and interlobulillar stroma tissues. Statistical analysis showed not significant differences (p> 0.05) between the three analysed glands. Numerous mast cells were located around salivary secretory ducts, in close association The results suggest that MT play a relevant role in salivary antigen detection and that there is a close cooperation with other antigen professional presenting cells like dendritic cells.
ABSTRACT
Dendritic cells (DCs) are the professional antigen-presenting cells responsible for initiating of the immune response. Langerhans cells (LCs) are a type of DC that is a permanent resident of the oral epithelium. LCs are organized conforming a network in such a way as to maximize their surface area for efficient apprehension of antigens. To detect age-related changes in the LCs network, fragments of gingival epithelium spontaneously accompanying dental removals were processed by immunohistochemistry. Monoclonal antibody CD1a followed by biotinized immunoglobulin-streptoavidin peroxidase were used to identify the LCs with the light microscope. LC density and LC types were analyzed according to their morphology and intraepithelial distribution. In the older age group (61-74 years) the density was significantly lower than in the younger age groups. Morphologically, LCs showed fewer dendritic-branching processes and had a rounded shape in the older age group. Present observations indicate that the LC network changes markedly with aging. These results suggest that immunological defense of the oral tissue might be compromised in old age.
