ABSTRACT
The effects of melatonin, aluminum oxide, and polymethylsiloxane complex on the expression of LYVE-1 (lymphatic vessel endothelial hyaluronan receptor) in the liver were studied in db/db mice with experimental obesity and type 2 diabetes mellitus. The complex or placebo was administered daily by gavage from week 8 to week 16 of life. The animals receiving the complex exhibited enhanced, in comparison with the placebo group, immunohistochemical LYVE-1+ staining of endothelial cells in sinusoids. Enhanced expression of LYVE-1 was associated with less pronounced dilatation of interlobular arteries, veins, and lymphatic vessels. Thee findings suggest a protective effect of the complex towards structural changes in the liver of mice with obesity and type 2 diabetes.
Subject(s)
Antioxidants/pharmacology , Diabetes Mellitus, Type 2/drug therapy , Glycoproteins/agonists , Hyperglycemia/drug therapy , Melatonin/pharmacology , Obesity/drug therapy , Aluminum Oxide/chemistry , Animals , Blood Glucose/metabolism , Diabetes Mellitus, Type 2/genetics , Diabetes Mellitus, Type 2/metabolism , Diabetes Mellitus, Type 2/pathology , Disease Models, Animal , Endothelial Cells/drug effects , Endothelial Cells/metabolism , Endothelial Cells/pathology , Female , Gene Expression/drug effects , Glycoproteins/genetics , Glycoproteins/metabolism , Hepatic Artery/drug effects , Hepatic Artery/metabolism , Hepatic Artery/pathology , Homozygote , Hyperglycemia/genetics , Hyperglycemia/metabolism , Hyperglycemia/pathology , Liver/drug effects , Liver/metabolism , Liver/pathology , Lymphatic Vessels/drug effects , Lymphatic Vessels/metabolism , Lymphatic Vessels/pathology , Membrane Transport Proteins , Mice , Mice, Transgenic , Obesity/genetics , Obesity/metabolism , Obesity/pathology , Receptors, Leptin/deficiency , Receptors, Leptin/genetics , Silicones/chemistryABSTRACT
Effect of the dipeptidyl peptidase-4 inhibitor linagliptin on structural manifestations of diabetic nephropathy was studied in BKS.Cg-Dock7m+/+Leprdb/J mice (experimental model of type 2 diabetes mellitus). Linagliptin (10 mg/kg per day) or vehicle was administered by gavage over 8 weeks. Mesangial expansion, thickening of the basement membrane in glomerular capillaries and proximal tubules, and retraction of cytopodia were less pronounced in mice receiving linagliptin. The protective effect of linagliptin on the kidney structure was not associated with its hypoglycemic action.
