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1.
J Am Mosq Control Assoc ; 39(3): 200-202, 2023 Sep 01.
Article in English | MEDLINE | ID: mdl-37796733

ABSTRACT

Culicoides species adults were collected in light traps located on the coast of Elota, Sinaloa, Mexico, in February 2022. All specimens were females, and it was determined based on their morphology that they belonged to the Culicoides variipennis complex. The identification of the species was carried out by means of a comparative analysis of genetic sequences of the cytochrome c oxidase subunit I gene, which resulted in C. occidentalis, this being its first report in Sinaloa and its third nationwide.


Subject(s)
Ceratopogonidae , Animals , Female , Male , Mexico
2.
Vector Borne Zoonotic Dis ; 23(12): 639-644, 2023 Dec.
Article in English | MEDLINE | ID: mdl-37651186

ABSTRACT

Background: Developing methods for the isolation and replication of dengue virus (DENV), based on nonhematophagous insect models to assess virus-host interaction, would contribute, for instance, to the creation of drugs or vaccines and eventually to the control of the disease. In this regard, nonhematophagous mosquitoes have been used as biological hosts for the isolation of DENV because they are specific and sensitive to a low viral load and viral particles with low infectivity. However, implementation of these models is mainly affected by the complexity of the establishment of the entomological colonies. Materials and Methods: In this study, the susceptibility of DENV-4 infection in Plodia interpunctella larvae was evaluated. Ten larvae, previously inoculated with supernatant from DENV-4-infected C6/36 cells, were processed to determine viral replication by the optical density and 2-ΔΔCt methods at different time intervals (1 and 7 days postinoculation). Results: A prospective increase in viral replication was observed, which did not influence the survival and development of P. interpunctella. Conclusion: These results demonstrate the infectivity of DENV-4 in P. interpunctella, thus becoming an option as a biological model for the study of this etiological agent.


Subject(s)
Aedes , Culicidae , Dengue Virus , Dengue , Moths , Animals , Larva , Prospective Studies , Dengue/veterinary , Virus Replication
3.
J Am Mosq Control Assoc ; 37(3): 161-163, 2021 09 01.
Article in English | MEDLINE | ID: mdl-34407163

ABSTRACT

Mosquito larvae were collected in the urban area of the city of Culiacan, Sinaloa, in September of 2020. The immature stages were placed in emergence containers and fed with Aedes aegypti larvae. The adults that emerged from the immature stages were mounted on insect pins and characterized based on their morphology. The species corresponded morphologically to Toxorhynchites moctezuma, making this the first report of the species for the state of Sinaloa, Mexico. Similarities and morphological variations are discussed with previous analysis for this species.


Subject(s)
Aedes , Animals , Cities , Larva , Mexico
4.
Vector Borne Zoonotic Dis ; 21(6): 478-481, 2021 06.
Article in English | MEDLINE | ID: mdl-33945340

ABSTRACT

Dengue virus (DENV) is transmitted to humans by the bite of the vector Aedes aegypti. Several researchers have suggested that the mechanism of vertical transmission of DENV in the vector is a key aspect for the prevalence of the virus in the environment and the potentiation of epidemic outbreaks of the disease. In this context and as part of an integrated study of DENV serotypes in mosquitoes of urban areas in Sinaloa, Mexico, the presence of DENV-4 in larval stages of Ae. aegypti was evaluated to demonstrate the vertical transmission of this serotype. In total, 672 larvae of Ae. aegypti were collected in 16 sectors and were grouped into 36 pools, of which 41.66% (15/36 pools) tested positive for DENV-4, with a minimum infection rate = 22.32. The analysis of the obtained sequences showed a 98% similarity to the DENV-4 with sequences previously reported in GenBank. These results show that Ae. aegypti acts as a natural reservoir for DENV-4 in this region.


Subject(s)
Aedes , Dengue Virus , Dengue , Animals , Dengue/epidemiology , Dengue/veterinary , Larva , Mexico/epidemiology , Mosquito Vectors , Serogroup
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