[Novel site-specific endonuclease from Acinetobacter species M strain]. / Novaia sait-spetsificheskaia éndonukleaza iz shtamma Acinetobacter species M.

Site-specific endonuclease R. AspMI was isolated and purified to apparent functional homogeneity from Acinetobacter species (strain M). The enzyme recognizes symmetrical DNA sequence 5'-AGG decreases CCT-3' and cleaves it at the site indicated by the arrow forming blind DNA ends. The endonuclease is an isoschizomer of the StuI endonuclease. Cleavage of the DNA site was inhibited by dcm-methylation. AspMI is approximately equal to 30 kD monomer.

[A new site-specific adenine DNA-methyltransferase from Bacillus species ST5]. / Novaia sait-spetsificheskaia adeninovaia DNK-metiltransferaza iz shtamma Bacillus species ST5.

The site-specific DNA-methylase M.BspST5I has been isolated from Bacillus species ST5 and purified to functional purity. M.BspST5I protects DNA from endonuclease R.BspST5I which recognizes the nonpalindromic sequence [formula: see text] on DNA. MpBspST5I belongs to adenine-specific methylases.

[A new site-specific endonuclease from Bacillus species ST5]. / Novaia sait-spetsificheskaia éndonukleaza iz Bacillus species ST5.

A site-specific endonuclease R.BspST5I has been isolated in a functionally pure state from the thermophilic strain of Bacillus species ST5. The enzyme recognizes sequence 5'-GCATC-3' on the DNA and splits it at a distance of five nucleotides from the 3'-end of the recognition site as well as at distances of nine or ten nucleotides at the complementary filament depending on the hydrolyzed sequence of the DNA. The enzyme is a isomer of endonuclease SfaNI from Streptococcus faecalis ND547.

[Site-specific endonuclease and methylase from thermophilic bacteria of Bacillus species IS4]. / Sait-spetsificheskie éndonukleaza i metilaza iz termofil'noi bakterii Bacillus species IS4.

The site-specific endonuclease R . BspIS4I and methylase M . BspIS4I have been isolated and purified to functional purity from the thermophilic strain of Bacillus species IS4. R . BspIS4I recognizes sequence [sequence: see text] on the DNA and cleaves it as indicated by the arrows to form single-stranded 4-nucleotide 5'-protruding termini. The enzyme is an isoschizomer of BbvII. M . BspIS4I is related to adenine-specific methylase.

[A new thermophilic strain of Bacillus coagulans--a producer of BcoK1 site-specific endonuclease]. / Novyi termofil'nyi shtamm Bacillus coagulans--produtsent sait- spetsificheskoi éndonukleazy BcoKI.

The strain, producing new site-specific endonuclease BcoKI has been found at the screening of the thermophilic bacteria isolated from tobacco. A phenotype characteristic of the strain is given. It has been identified as a new strain Bacillus coagulans. BcoKI, a class-IIS restriction endonuclease has been obtained by three consecutive chromatographies on blue agarose hydroxyapatite and heparin-Sepharose. BcoKI, an isoschizomer of Ksp6321, recognizes the six base non-palindromic sequence 5'CTCTTC3' and cleaves one nucleotide 3' of the 3' cytosine on this strand and four nucleotide 5' of the 5' guanine on the opposite strand to generate a three base 5' overhang.

[Isolation and properties of site-specific endonuclease BspTS514I from the thermophilic bacteria Bacillus species TS514]. / Vydelenie i svoistva sait-spetsificheskoi éndonukleazy BspTS514I iz termofil'noi bakterii Bacillus species TS514.

New site-specific endonucleases BspBS31I, BstBS32I, BspIS41, BstTS5I, BspTS514I were isolated from five thermophilic soil bacteria Bacillus sp. BS31, B. stearothermophilus BS32, Bacillus sp. IS4, B. stearothermophilus TS5, Bacillus sp. TS514. The enzymes are isoschizomers of the restriction endonuclease BbvII. Endonuclease BspTS514I was obtained pure from interfering contaminations by two consecutive chromatographies on blue agarose and hydroxyapatite. The enzyme exhibits a maximal activity at 55 degrees C in 10 mM tris-HCl (pH 9.2), 10 mM MgCl2 and 50 mM NaCl.