ABSTRACT
Objective: Erchen Decoction (ECD), a well-known traditional Chinese medicine, exerts metabolism-regulatory, immunoregulation, and anti-tumor effects. However, the action and pharmacological mechanism of ECD remain largely unclear. In the present study, we explored the effects and mechanisms of ECD in the treatment of CRC using network pharmacology, molecular docking, and systematic experimental validation. Methods: The active components of ECD were obtained from the TCMSP database and the potential targets of them were annotated by the STRING database. The CRC-related targets were identified from different databases (OMIM, DisGeNet, GeneCards, and DrugBank). The interactive targets of ECD and CRC were screened and the protein-protein interaction (PPI) networks were constructed. Then, the hub interactive targets were calculated and visualized from the PPI network using the Cytoscape software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. In addition, the molecular docking was performed. Finally, systematic in vitro, in vivo and molecular biology experiments were performed to further explore the anti-tumor effects and underlying mechanisms of ECD in CRC. Results: A total of 116 active components and 246 targets of ECD were predicted based on the component-target network analysis. 2406 CRC-related targets were obtained from different databases and 140 intersective targets were identified between ECD and CRC. 12 hub molecules (STAT3, JUN, MAPK3, TP53, MAPK1, RELA, FOS, ESR1, IL6, MAPK14, MYC, and CDKN1A) were finally screened from PPI network. GO and KEGG pathway enrichment analyses demonstrated that the biological discrepancy was mainly focused on the tumorigenesis-, immune-, and mechanism-related pathways. Based on the experimental validation, ECD could suppress the proliferation of CRC cells by inhibiting cell cycle and promoting cell apoptosis. In addition, ECD could inhibit tumor growth in mice. Finally, the results of molecular biology experiments suggested ECD could regulate the transcriptional levels of several hub molecules during the development of CRC, including MAPKs, PPARs, TP53, and STATs. Conclusion: This study revealed the potential pharmacodynamic material basis and underlying molecular mechanisms of ECD in the treatment of CRC, providing a novel insight for us to find more effective anti-CRC drugs.
ABSTRACT
In the optical system for reconstructing speech signals based on laser speckle images, the resonance between the sound source and nearby objects leads to frequency response problem, which seriously affects the accuracy of reconstructed speech. In this paper, we propose a speech enhancement algorithm to reduce the frequency response. The results show that after using the speech enhancement algorithm, the frequency spectrum correlation coefficient between the reconstructed sinusoidal signal and the original sinusoidal signal is improved by up to 82.45%, and the real speech signal is improved by up to 56.40%. This proves that the speech enhancement algorithm is a valuable tool for solving the frequency response problem and improving the accuracy of reconstructed speech.
Subject(s)
Optical Devices , Speech , Algorithms , LasersABSTRACT
This study is focused on numerical simulation analysis and experimental study regarding the influence of backscattering characteristics of objects for long-range laser voice acquisition. Based on theoretical analysis, three parameters, including surface roughness of an object, incident angle, and refractive index, which will influence the performance of remote scattered reflection laser interference voice acquisition, are investigated and analyzed. After analysis and simulation, an experimental system is set up to demonstrate the influence of backscattering characteristics of an object. The results show that the restored amplitude of a voice signal decreases gradually, corresponding to an increase of surface roughness from 0.4 to 12.5 µm; the incident angle of the measured laser shall reside between 57.32 deg (1 rad) and -57.32 deg (-1 rad); the optimal incident angle is 0 deg for all kinds of objects; and the metal object is a better choice of material selection. In addition, the restored amplitude of a voice signal rises with the attenuation coefficient of metal increasing. It also increases with the refractive index for a nonmetallic object. Comparing a metal to a nonmetallic object, the amplitude of voice signal varies significantly.
ABSTRACT
In this paper we propose a novel, improved, phase generated carrier (PGC) demodulation algorithm based on the PGC-differential-cross-multiplying approach (PGC-DCM). The influence of phase modulation amplitude variation and light intensity disturbance (LID) on traditional PGC demodulation algorithms is analyzed theoretically and experimentally. An experimental system for remote no-contact microvibration measurement is set up to confirm the stability of the improved PGC algorithm with LID. In the experiment, when the LID with a frequency of 50 Hz and the depth of 0.3 is applied, the signal-to-noise and distortion ratio (SINAD) of the improved PGC algorithm is 19 dB, higher than the SINAD of the PGC-DCM algorithm, which is 8.7 dB.
