ABSTRACT
The aim of this article is to study the relationship between GSTM1 polymorphism and colon cancer and to compare the chromosomal breakage induced by mutagen in a colon cancer group and healthy controls. Using PCR to identify the GSTM1 genotype, we found the frequency of GSTM1- in colon cancer (n = 19) and control group (n = 23) was 36.8% and 26.1%, respectively (p > 0.05, chi 2-test). The bleomycin-induced chromosomal breakage (break/cell) in the patient group was 0.75 +/- 0.29, and in the control group 0.42 +/- (0.24) (p < 0.05, t-test). The percentage of mutagen sensitivity (b/c > 0.8) in the patient group (68%) was 4 times as high as that in the control group (20%). The mutagen hypersensitivity (b/c > 1.0) in the patient group (47%) was 5 times as high as that in the control group (12%). The odds radio was 6.6.
Subject(s)
Colonic Neoplasms/epidemiology , Colonic Neoplasms/genetics , Glutathione Transferase/genetics , Mutagens/pharmacology , Polymorphism, Genetic , Adult , Aged , Antibiotics, Antineoplastic/pharmacology , Bleomycin/pharmacology , China , Chromosome Aberrations , Colonic Neoplasms/drug therapy , DNA Damage/drug effects , DNA Damage/genetics , Drug Resistance, Neoplasm/genetics , Female , Gene Deletion , Gene Frequency , Glutathione Transferase/drug effects , Humans , Lymphocytes/drug effects , Male , Middle AgedABSTRACT
The Han people in Guangong province were studied to determine the frequencies of BclI, XbaI, and BglI restriction fragment length polymorphisms (RFLPs) in FVIII:C gene. The incidences for positive BclI, XbaI, and BglI sites were 63.5%, 43.5%, and 100%, respectively. Linkage of BclI and XbaI RFLPs were studied and the result showed that 19.5 percent of positive BclI polymorphic site homozygotes were heterozygous for the XbaI RFLP. Hence combining these two RFLPs was informative for 65.9 percent of females. We implemented carrier detection in two hemophilia A families and prenatal diagnosis in another hemophilia A family with these RFLPs.
