Occurrence and mechanism of sulfamethoxazole in alginate-like extracellular polymers from excess sludge.

The recovery of biopolymers, particularly alginate-like extracellular polymers, from municipal sludge represents a promising step toward sustainable sludge treatment practices. Originating from wastewater plants in complexly polluted environments, alginate-like extracellular polymers carry potential environmental risks concerning their reuse. This study employs ultrahigh-performance liquid chromatography-tandem mass spectrometry to investigate the distribution coefficients and occurrence of alginate-like extracellular polymers and sulfamethoxazole. Results demonstrate a negative distribution coefficient, suggesting an inhibitory effect on sulfamethoxazole dissolution. The ethanol-extracted alginate-like extracellular polymers exhibits higher sulfamethoxazole levels (approximately 52%) than those obtained via dialysis extraction. Three-dimensional excitation-emission matrix analysis and adsorption studies indicate the absence of tyrosine-like substances in the alginate-like extracellular polymers, unlike in other extracellular polymeric substances. This absence diminishes hydrophobic interactions, highlighting that electrostatic interactions play a more important role. These insights are crucial for understanding the adsorption behavior of alginate-like extracellular polymers and optimizing their large-scale extraction processes.

Extracting compositional blocks of alginate-like extracellular polymers (ALE) from conventional activated sludge (CAS).

As a highly added value material, alginate-like extracellular polymers (ALE) can be extracted from extracellular polymeric substances (EPS) from aerobic granular sludge (AGS). In fact, conventional activated sludge (CAS) also contains a certain amount of ALE. As CAS is widely used everywhere, waste activated sludge (WAS) from CAS is huge in its absolute amount. Although the ALE property of CAS was identified not so good as that from AGS, the mechanisms remains unclear. For this reason, it is necessary to unravel the chemically compositional blocks of ALE. Referring to natural alginate, ALE can be separated into three compositional blocks: GGL, GML and MML (like units containing guluronate or mannuronate), associated with other compositions including protein (PN), polysaccharide (PS), phosphorus (P), humic acid (HA). With real WAS from CAS, ALE was extracted and three blocks were separated: GGL = 54 %, GML = 42 % and MML = 4 % in weight, which is similar to the previous study. Moreover, the GGL blocks in CAS were obviously lower than AGS, down to by 1/3-1/2. And the GML and MML blocks in CAS were much higher than AGS, by more than 1/2. Different compositional blocks of ALE in AGS and CAS should be the reason forming different properties in applications. For this reason, a further study will be initiated to dispense/reorganize three blocks of ALE from CAS for expanding its potential applications, based on the compositional blocks of ALE from AGS.