SlTrxh functions downstream of SlMYB86 and positively regulates nitrate stress tolerance via S-nitrosation in tomato seedling.

Nitric oxide (NO) is a redox-dependent signaling molecule that plays a crucial role in regulating a wide range of biological processes in plants. It functions by post-translationally modifying proteins, primarily through S-nitrosation. Thioredoxin (Trx), a small and ubiquitous protein with multifunctional properties, plays a pivotal role in the antioxidant defense system. However, the regulatory mechanism governing the response of tomato Trxh (SlTrxh) to excessive nitrate stress remains unknown. In this study, overexpression or silencing of SlTrxh in tomato led to increased or decreased nitrate stress tolerance, respectively. The overexpression of SlTrxh resulted in a reduction in levels of reactive oxygen species (ROS) and an increase in S-nitrosothiol (SNO) contents; conversely, silencing SlTrxh exhibited the opposite trend. The level of S-nitrosated SlTrxh was increased and decreased in SlTrxh overexpression and RNAi plants after nitrate treatment, respectively. SlTrxh was found to be susceptible to S-nitrosation both in vivo and in vitro, with Cysteine 54 potentially being the key site for S-nitrosation. Protein interaction assays revealed that SlTrxh physically interacts with SlGrx9, and this interaction is strengthened by S-nitrosation. Moreover, a combination of yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR), and transient expression assays confirmed the direct binding of SlMYB86 to the SlTrxh promoter, thereby enhancing its expression. SlMYB86 is located in the nucleus and SlMYB86 overexpressed and knockout tomato lines showed enhanced and decreased nitrate stress tolerance, respectively. Our findings indicate that SlTrxh functions downstream of SlMYB86 and highlight the potential significance of S-nitrosation of SlTrxh in modulating its function under nitrate stress.

Overexpression of tomato glutathione reductase (SlGR) in transgenic tobacco enhances salt tolerance involving the S-nitrosylation of GR.

S-nitrosylation, a post-translational modification (PTM) dependent on nitric oxide, is essential for plant development and environmental responsiveness. However, the function of S-nitrosylation of glutathione reductase (GR) in tomato (SlGR) under NaCl stress is yet uncertain. In this study, sodium nitroprusside (SNP), an exogenous NO donor, alleviated the growth inhibition of tomato under NaCl treatment, particularly at 100 µM. Following NaCl treatment, the transcripts, enzyme activity, and S-nitrosylated level of GR were increased. In vitro, the SlGR protein was able to be S-nitrosylated by S-nitrosoglutathione (GSNO), significantly increasing the activity of GR. SlGR overexpression transgenic tobacco plants exhibited enhanced germination rate, fresh weight, and increased root length in comparison to wild-type (WT) seedlings. The accumulation of reactive oxygen species (ROS) was lower, whereas the expression and activities of GR, ascorbate peroxidase (APX), superoxide dismutase (SOD), and catalase (CAT); the ratio of ascorbic acid/dehydroascorbic acid (AsA/DHA), reduced glutathione/oxidized glutathione (GSH/GSSG), total soluble sugar and proline contents; and the expression of stress-related genes were higher in SlGR overexpression transgenic plants in comparison to the WT plants following NaCl treatment. The accumulation of NO and S-nitrosylated levels of GR in transgenic plants was higher in comparison to WT plants following NaCl treatment. These results indicated that S-nitrosylation of GR played a significant role in salt tolerance by regulating the oxidative state.

Synergistic effects of biochar and carboxymethyl cellulose sodium (CMC) applications on improving water retention and aggregate stability in desert soils.

Making improvements to the water-holding characteristics and water-erosion resistance of desert soils, particularly in inland extremely arid areas, is vital for achieving both sustainable water resource utilisation and food security. The aim of this study is to evaluate the effects of the co-application of biochar and carboxymethyl cellulose sodium (CMC) on the physical properties of sandy desert soil, including infiltration rate, saturated water conductivity, field water-holding capacity and aggregate stability. Sandy desert soil samples were collected from jujube plantations on the southern edge of the Taklimakan Desert in the Hotan Prefecture, Xinjiang, China. Five CMC application ratios (C0:0, C1:0.01 g/kg, C2:0.02 g/kg, C3:0.04 g/kg and C4:0.08 g/kg) and five biochar application ratios (B0:0, B1:1.0 g/kg, B2:2.0 g/kg, B3:4.0 g/kg and B4:8.0 g/kg) were designed and a total of 11 experimental treatments were performed, which were labelled as CK (control group), B2C0, B2C1, B2C2, B2C3, B2C4, B4C4, B0C2, B1C2, B3C2 and B4C2. Compared with CK, the combined application of biochar and CMC reduced the soil bulk density (BD) by 1.29-9.41% and the saturated hydraulic conductivity (Ks) by 29.64-94.98%, and increased the soil saturated water content (SSWC) by 8.81-30.74% and the water holding capacity (WHC) by 13.91-36.87%. Similarly, the water-stable aggregates that were co-applied with biochar and CMC increased by 29.10-256.86%. This resulted in significant improvement in the stability of sandy desert soil against water erosion. The principal component analysis (PCA) results found B4C4 to have the best comprehensive improvement effect. Therefore, 0.08 g/kg of CMC and 8.0 g/kg of biochar were used as recommended for improving the hydraulic properties of desert soils. Generally, CMC and biochar have a mutually complementary effect on improving sandy desert soil, providing new ideas and approaches for the improvement of soil and the sustainable development of agriculture in desert areas.