1.
2.
Chin Med J (Engl)
; 132(24): 3021-3023, 2019 Dec 20.
Article
in English
| MEDLINE
| ID: mdl-31833908
Subject(s)
Coronary Disease/etiology , Depression/complications , Disease Models, Animal , Animals , Chronic Disease , Diet, High-Fat , Rats , Rats, Wistar
3.
Theriogenology
; 132: 22-26, 2019 Jul 01.
Article
in English
| MEDLINE
| ID: mdl-30981942
ABSTRACT
Adenovirus is a kind of non-enveloped,double-stranded DNA virus. As a member of the mammalian adenoviruses of the Adenoviridae family, porcine adenovirus causes gastrointestinal disease in piglets. In this study, the modified adenovirus was manipulated to carry a green fluorescence EGFP marker. The modified adenovirus was added to medium199 for co-incubation or microinjected into the cytoplasm of porcine oocytes. The effect of adenovirus on the first polar body extrusion was not significant during porcine oocyte maturation. Our data demonstrated the zona pellucida plays a vital role in porcine oocytes being resistant to modified adenovirus. Additionally, the results suggested that oocytes protect themselves from nonself substances.