Influence of sinomenine on protein profiles of peripheral blood mononuclear cells from ankylosing spondylitis patients: a pharmacoproteomics study.

BACKGROUND: Ankylosing spondylitis (AS) is a common inflammatory rheumatic disease which lacks satisfactory treatment so far. Sinomenine (SIN) is an alkaloid and has recently been utilized in treating multiple rheumatic diseases including AS in China, but its exact mechanism remains to be explored. This study investigated the alteration of proteome in peripheral blood mononuclear cells (PBMCs) from AS patients. METHODS: Thirty AS patients were enrolled in this study. PBMCs from each AS patient were cultured in medium with or without SIN respectively. Then PBMCs proteins from both groups were separated by two-dimensional electrophoresis (2-DE) and analyzed by mass spectrometry (MS). Two differentially expressed proteins were then chosen to be verified using Western blotting. RESULTS: Seven proteins, including a-synuclein (SNCA), calmodulin (CALM), acidic leucine-rich nuclear phosphoprotein 32 family member A (ANP32A), chloride intracellular channel protein 1 (CLIC1), guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (GNB1), gelsolin (GSN) and histone H2B type 1-M (HISTH2BM) were over-expressed, while coronin- 1A (CORO1A) was under-expressed in the SIN-treated PBMCs. Further bioinformatics search indicated that the changes of SNCA, ANP32A and CLIC1 pertained to apoptosis, while changes of GSN and CORO1A were associated with both apoptosis and inhibition of immunological function. Subsequently GSN and CORO1A were selected to validate by Western blotting and the results were consistent with those of 2-DE. CONCLUSION: There were 8 differentially expressed proteins in the SIN-treated PBMCs, which might shed some light on the mechanism of SIN in the treatment of AS.

[Clinical study of the new intelligent determination and analysis system for glomerular filtration rate].

OBJECTIVE: To discuss the clinical application value of determining glomerular filtration rate(GFR) with the new intelligent determination and analysis system for GFR (B&G System). METHODS: GFR of 216 hospitalized patients suffering from the different diseases was determined accurately by clearance rate of (99m)Tc-diethylene triamine pentaacetic acid ((99m)Tc-DTPA) (Tc-GFR), and the serum creatinine (SCr) and blood urea nitrogen (BUN) were also determined. At the same time GFR was determined by B&G system and Robert formula (B&G-GFR, Robert-GFR), and creatinine clearance rate (CCr) and GFR were calculated by Cockcroft/Gault formula(CG-CCr-GFR). All the results were compared, and correlation analysis was done for the three groups of data. RESULTS: Among 216 patients, B&G-GFR and Tc-GFR in 106 patients with renal insufficiency and 110 patients with normal renal function showed no significant difference (ml · min(-1)· 1.73 m(-2): 29.13 ± 18.96 vs. 32.28 ± 25.03, 121.13 ± 49.79 vs. 118.48 ± 43.25, both P >0.05), while Robert-GFR (ml · min(-1) · 1.73 m(-2): 21.45 ± 15.67, 93.54 ± 30.01) and CG-CCr-GFR (ml · min(-1) · 1.73 m(-2): 11.87 ± 8.69, 86.27 ± 21.44)were significantly lower than Tc-GFR (P<0.05 or P <0.01 ). The values of B&G-GFR, Robert-GFR, CG-CCr-GFR and Tc-GFR in renal insufficiency group (ml · min(-1) · 1.73 m(-2) :6.15 ± 14.07, 13.83 ± 11.36, 23.41 ± 24.34) were respectively significantly lower than that of normal renal function group (ml · min(-1) · 1.73 m(-2):7.65 ± 6.54, 24.94 ± 13.24, 32.21 ± 21.81, all P <0.05). B&G-GFR, Robert-GFR, and CG-CCr-GFR of both groups were positively correlated with Tc-GFR (P<0.05 or P <0.01), but negatively correlated with SCr (P<0.05 or P <0.01). CONCLUSION: B&G-GFR, Robert-GFR and CG-CCr-GFR , to a certain extent, do accurately reflect GFR. However, B&G-GFR is more accurate than others, and it can take the place of Tc-GFR clinically. B&G system has the advantage of accuracy, simplicity, speed, safety and low-price compared with other methods for the determination of GFR, and it warrants popularization.