ABSTRACT
In this study, we characterized the intratumoral expression of IL-17 and CD8(+) TILs in gastric adenocarcinoma patients after resection and determined the correlation between the survival probability of gastric adenocarcinoma patients and the expression of IL-17 in tumor. Expression of IL-17 and CD8 was assessed by immunohistochemistry, and the prognostic effects of intratumoral IL-17 expression and CD8(+) TILs were evaluated by Cox regression and Kaplan-Meier analysis. Immunohistochemical detection revealed the presence of IL-17 and CD8(+) cells in gastric adenocarcinoma tissue samples (90.6%, 174 out of 192 patients and 96.9%, 186 out of 192 patients, respectively). We have also found that intratumoral IL-17 expression was significantly correlated with age (p=0.004) and that the number of CD8(+)TILs was significantly correlated with UICC staging (p=0.012) and the depth of tumor invasion (p=0.022). The five-year overall survival probability among patients intratumorally expressing higher levels of IL-17 was significantly better than those expressing lower levels of IL-17 (p=0.036). Multivariate Cox proportional hazard analyses revealed that intratumoral IL-17 expression (HR: 0.521; 95% CI: 0.329-0.823; p=0.005) was an independent factor affecting the five-year overall survival probability. We conclude that low levels of intratumoral IL-17 expression may indicate poor prognosis in gastric adenocarcinoma patients.
Subject(s)
Adenocarcinoma/diagnosis , Interleukin-17/metabolism , Stomach Neoplasms/diagnosis , Adenocarcinoma/metabolism , Adenocarcinoma/mortality , Adolescent , Adult , Age Factors , Aged , Aged, 80 and over , CD8 Antigens/metabolism , Female , Humans , Immunohistochemistry , Kaplan-Meier Estimate , Male , Middle Aged , Predictive Value of Tests , Prognosis , Stomach Neoplasms/metabolism , Stomach Neoplasms/mortalityABSTRACT
The principle of optic fiber surface plasmon resonance sensor is discussed in detail based on the electromagnetic theory and radial theory in the present paper. The relationship between SPR spectrum resonance wavelength and liquid refractive index is also analyzed. Through relative spectrum measurement technology, SPR spectrum curves, whose resonance wavelength increases with increasing liquid refractive index, were obtained for eight different kinds of chemical liquids. The measurement of concentration of mixed solution including ethanol and glycol was performed by analyzing the spectrum attribute. The experimental results show that the sensing system features simple structure and all light transmitted by fiber. It not only realizes rapid, accurate and real time measurement for target medium, but also can be used in some special situations and easily realize long distance measurement.
