ABSTRACT
AIM: To examine the role of Fibrinogen-like protein 2 (fgl2)/fibroleukin in tumor development. Fgl2 has been reported to play a vital role in the pathogenesis in MHV-3 (mouse hepatitis virus) induced fulminant and severe hepatitis, spontaneous abortion, allo- and xeno- graft rejection by mediating "immune coagulation". METHODS: Tumor tissues from 133 patients with six types of distinct cancers and the animal tumor tissues from human hepatocellular carcinoma (HCC) model on nude mice (established from high metastasis HCC cell line MHCC97LM6) were obtained. RESULTS: Hfgl2 was detected in tumor tissues from 127 out of 133 patients as well as tumor tissues collected from human HCC nude mice. Hfgl2 was highly expressed both in cancer cells and interstitial inflammatory cells including macrophages, NK cells, and CD8(+) T lymphocytes and vascular endothelial cells. Hfgl2 mRNA was localized in cells that expressed hfgl2 protein. Fibrin (nogen) co-localization with hfgl2 expression was determined by dual immunohistochemical staining. In vitro, IL-2 and IFN-gamma increased hfgl2 mRNA by 10-100 folds and protein expression in both THP-1 and HUVEC cell lines. One-stage clotting assays demonstrated that THP-1 and HUVEC cells expressing hfgl2 had increased procoagulant activity following cytokines stimulation. CONCLUSION: The hfg12 contributes to the hypercoagulability in cancer and may induce tumor angiogenesis and metastasis via cytokine induction.
Subject(s)
Carcinoma, Hepatocellular/metabolism , Fibrinogen/metabolism , Interferon-gamma/metabolism , Interleukin-2/metabolism , Liver Neoplasms/metabolism , Thrombophilia/metabolism , Thromboplastin/metabolism , Adult , Aged , Animals , Carcinoma, Hepatocellular/pathology , Cell Line , Cell Line, Tumor , Disease Models, Animal , Endothelial Cells/metabolism , Endothelial Cells/pathology , Female , Humans , Leukemia, Monocytic, Acute/metabolism , Leukemia, Monocytic, Acute/pathology , Liver Neoplasms/pathology , Male , Mice , Mice, Inbred BALB C , Mice, Nude , Middle Aged , RNA, Messenger/metabolismABSTRACT
<p><b>OBJECTIVE</b>To explore the impact of different warm ischemia time on structure and function of the non-heart-beating donor lung and to find out the feasibility of non-heart-beating donor in rat lung transplantation.</p><p><b>METHODS</b>Sixty Sprague-Dawley rats were randomly divided into 3 groups: heart-beating donor (HBD) group, non-heart-beating donor (NHBD) with 30 minutes of warm ischemia time (WIT) group and NHBD with 60 minutes of WIT group. Each group has 10 pairs (the donors and the recipients). The donor lungs of group HBD were flushed with low potassium dextran (LPD) solution at 4 degrees C after asystolia while the lungs of group NHBD-30 and group NHBD-60 remained ventilated at the room temperature for 30 and 60 minutes after asystolia and then were flushed with LPD solution. All the donor lungs remained inflated when they were stored in LPD solution at 4 degrees C for 4 hours. The recipient rat underwent left thoracotomy, and then orthotopic left lung transplantation. Followed by a right thoracotomy, the right pulmonary hilum were ligated with one-hour reperfusion and ventilation.</p><p><b>RESULTS</b>All the recipients in group HBD and group NHBD-30 survived the observation period of one hour with excellent gas exchange, whereas 4 of recipients in group NHBD-60 survived for 10 minutes after the ligation of right pulmonary hilum and 3 for 20 minutes. The pulmonary compliance, ultrastructure, energy metabolite and other markers revealed no significant differences between group HBD and group NHBD-30 (P > 0.05). But the differences between group NHBD-60 and other two groups were significant (P < 0.05).</p><p><b>CONCLUSIONS</b>The adoption of non-heart-beating donor could be a safe and effective method to expand the lung donor pool. The NHBD lung with 30 minutes of WIT may be suitable for lung transplantation in rat.</p>
Subject(s)
Animals , Male , Rats , Heart , Ischemia , Lung , Lung Transplantation , Microscopy, Electron , Models, Animal , Random Allocation , Rats, Sprague-Dawley , Time Factors , Tissue DonorsABSTRACT
OBJECTIVE: To investigate the efficacy and safety of the implantation of phakic anterior chamber intraocular lens for high myopia. METHODS: A consecutive group of 29 eyes in 16 patients with (-7.00 to -30.00) D of myopia were implanted. RESULTS: All of the 29 eyes were implanted successfully and followed-up 3 approximate, equals 6 months. The mean best corrected visual acuity was 0.50+/-0.26 pre-operatively and 0.73+/-0.263 months post-operatively, there was no significant difference t=2.043, P=0.051 . The mean refractive diopter was -18.03+/-5.54 D pre-operatively and -0.82+/-1.54 D post-operatively t=30.899 P=0.000 ; The mean intraocular pressure was 2.091+/-0.380 kPa pre-operatively and (1.734+/-0.572)kPa post-operatively t=1.98 P=0.07 ; The mean counts of endothelial cells was (2704+/-390 /mm2 pre-operatively and (2 519+/-278)/mm2 post-operatively (t=1.16 P=0.26). CONCLUSION: The implantation of phakic anterior chamber intraocular lens for high myopia is predictable, reversible and controllable with simple manipulation. Long- time following-up is still required.