ABSTRACT
This essay is a reflection and assessment of the ConFem and faculty collective's queer Chicanx/Latinx intergenerational solidarity activism. In conversation with abolition feminisms, transformative justice practices, and queer performance studies, we illustrate the shifts the collective effected toward queerer Chicanx/Latinx feminist futurities. Our collective solidarity praxis was an intervention that actively undermined the anti-solidarity machinations of the state's social hierarchical ordering at the site of the university. This essay addresses the collective's strategic move to shift away from supplicating or engaging with the state for appeasement or resolution of violence, and instead to turn to harnessing the power of queer Chicanx/Latinx visionary artists to unleash queer feminist Chicanx/Latinx counterpublics and imagination.
Subject(s)
Homosexuality, Female , Sexual and Gender Minorities , Female , Humans , Feminism , Hispanic or Latino , ImaginationABSTRACT
The present research was performed to isolate and study the effects of a low molecular weight (<1300Da) parasite-associated substance, obtained from peritoneal fluids of female mice infected with Taenia crassiceps cysticerci, on seminiferous epithelium cells of male mice testis. The results showed an intense disruption of Sertoli cells and germ cells within the seminiferous tubules of experimental mice, along with the destruction of their gap junction (GJ). Significant generalized apoptosis of germ cells within seminiferous tubules was determined by TUNEL staining (P=0.0159). In addition, a significant number of infiltrating macrophages were found in the luminal space of these seminiferous tubules (P<0.0001). Finally, electron microscopy studies revealed structural and morphological abnormalities in the somatic cells (Sertoli and Leydig cells) and in the germ cells, primarily in the round and elongate spermatids.
Subject(s)
Ascitic Fluid/chemistry , Cysticercosis/parasitology , Cysticercus/metabolism , Testis/pathology , Animals , Apoptosis , Ascitic Fluid/parasitology , Chromatography, Gel , Cysticercosis/immunology , Cysticercosis/pathology , Cysticercus/immunology , Electrophoresis, Polyacrylamide Gel , Female , In Situ Nick-End Labeling , Macrophages/pathology , Male , Mice , Mice, Inbred BALB C , Microscopy, Electron, Transmission , Molecular Weight , Seminiferous Epithelium/pathology , Seminiferous Epithelium/ultrastructure , Testis/ultrastructure , UltrafiltrationABSTRACT
This research was carried out to study the effects of infection with Taenia crassiceps cysticerci on the seminiferous epithelium histoarchitecture in the testes of male mice. Our results showed a severe disruption of the histoarchitecture of the testis epithelium in infected mice. In these animals, a significant infiltration of macrophages within seminiferous tubules was observed (P < 0.001). Generalized apoptosis of germ cells within the seminiferous tubules was observed, as assessed by TUNEL assay and apoptotic nuclei were quantified. The total number of fluorescent objects (DNA) (including clusters, singles, and objects in clusters) was significantly higher in the infected cells than in the control group (P = 0.0286). Observation of the interstitial tissue showed disorder and deterioration of many Leydig cells of infected mice, as well as intense vacuolization and destruction of their inter-cellular junctions. Several ultrastructural abnormalities were observed through electron microscopy as well. The observed pathology could lead to a state of infertility.
Subject(s)
Apoptosis , Seminiferous Tubules/pathology , Taenia/pathogenicity , Taeniasis/pathology , Acridine Orange , Animals , Coloring Agents , Disease Models, Animal , Fluorescent Dyes , In Situ Nick-End Labeling , Male , Mice , Mice, Inbred BALB C , Microscopy, Confocal , Microscopy, Electron, Transmission , Seminiferous Tubules/parasitology , Seminiferous Tubules/ultrastructure , Tolonium ChlorideABSTRACT
After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis.
