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1.
Insect Biochem Mol Biol ; 37(12): 1359-65, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17967354

ABSTRACT

The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from G. assimilis. Partial genomic sequence encoding the Jhe contains two large (1330 and 2918bp) introns. No coding DNA sequence variation was observed over a 1293bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F(2) progeny of crosses between them. Phylogenetic analyses localised the G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade.


Subject(s)
Carboxylic Ester Hydrolases/metabolism , Fat Body/enzymology , Gryllidae/enzymology , Hemolymph/enzymology , Amino Acid Sequence , Animals , Base Sequence , Carboxylic Ester Hydrolases/genetics , Carboxylic Ester Hydrolases/isolation & purification , Cloning, Molecular , Gastrointestinal Tract/enzymology , Gene Library , Gryllidae/chemistry , Gryllidae/genetics , Introns , Isoenzymes/genetics , Isoenzymes/isolation & purification , Isoenzymes/metabolism , Molecular Probe Techniques , Molecular Sequence Data , Phylogeny , Selection, Genetic , Sequence Analysis, DNA , Structure-Activity Relationship
2.
Evolution ; 55(3): 538-49, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11327161

ABSTRACT

The hormonal basis of variation in life-history traits is a poorly studied topic in life-history evolution. An important step in identifying the endocrine-genetic causes of life-history variation is documenting statistical and functional associations between hormone titers and genotypes/phenotypes that vary in life-history traits. To this end, we compared the blood ecdysteroid titer and the mass of the ovaries during the first week of adulthood among a flight-capable morph and two flightless morphs of the wing-polymorphic cricket Gryllus firmus. Ecdysteroids are a group of structurally related hormones that regulate many important aspects of reproduction in insects. Both the ecdysteroid titer and ovarian mass were significantly higher in each of two flightless morphs compared with the flight-capable morph throughout the first week of adulthood. Genetically based differences in the ecdysteroid titer and ovarian mass between morphs from different selected lines were similar to phenotypically based differences among morphs from the same control (unselected) lines. By day 7 of adulthood, ovaries were typically 200-400% larger and the ecdysteroid titer was 60-300% higher in flightless versus the flight-capable morph. In addition, highly significant, positive, phenotypic correlations were observed between the ecdysteroid titer and ovarian mass in pooled samples of the two flightless and flight-capable crickets from control lines or from selected lines. The ecdysteroid titer was sufficiently elevated in the flightless morphs to account for their elevated ovarian growth. This is the first direct documentation that naturally occurring phenotypes/genotypes that differ in early fecundity, a key life-history trait, also differ phenotypically and genetically in the titer of a key reproductive hormone that potentially regulates that trait.


Subject(s)
Genetic Variation , Gryllidae/genetics , Steroids/blood , Wings, Animal/physiology , Animals , Ecdysteroids , Female , Gryllidae/metabolism , Gryllidae/physiology , Hemolymph/metabolism , Male , Organ Size/genetics , Ovary/anatomy & histology , Ovary/physiology , Reproduction , Wings, Animal/growth & development
3.
Physiol Biochem Zool ; 74(2): 293-306, 2001.
Article in English | MEDLINE | ID: mdl-11247748

ABSTRACT

The wing-polymorphic cricket, Gryllus firmus, contains (1) a flight-capable morph (LW(f)) with long wings and functional flight muscles, (2) a flightless morph with reduced wings and underdeveloped flight muscles (SW), and (3) a flightless morph with histolyzed flight muscles but with fully developed wings (LW(h)). The LW(f) morph differed genetically from the SW morph and phenotypically from the LW(h) morph in the size of flight muscles, ovarian growth during the first week of adulthood, and the hemolymph titer of juvenile hormone (JH). This is the first study to document that phenotypes that differ genetically in morphological aspects of dispersal capability and in ovarian growth also differ genetically in the titer of a hormone that potentially regulates those traits. The JH titer rose 9-100-fold during the photophase in the flight-capable LW(f) morph but did not change significantly during this time in either flightless morph. Prolonged elevation of the in vivo JH titer in flight-capable females, by topical application of a hormone analogue, caused a substantial increase in ovarian growth and histolysis of flight muscles. The short-term, diurnal rise in the JH titer in the dispersing morph may be a mechanism that allows JH to positively regulate nocturnal flight behavior, while not causing maladaptive histolysis of flight muscles and ovarian growth. This is the first demonstration of naturally occurring, genetically based variation for diurnal change in a hormone titer in any organism.


Subject(s)
Biological Evolution , Circadian Rhythm , Gryllidae/physiology , Juvenile Hormones/genetics , Juvenile Hormones/physiology , Animals , Genotype , Gryllidae/genetics , Phenotype , Polymorphism, Genetic
4.
J Insect Physiol ; 47(11): 1337-1347, 2001 Nov.
Article in English | MEDLINE | ID: mdl-12770186

ABSTRACT

The flight-capable morph of the wing-polymorphic cricket, Gryllus firmus, exhibited significantly higher activities of each of five lipogenic enzymes compared with the obligately flightless morph on a standard and a high-carbohydrate diet during early adulthood. Similarly, the rate of incorporation of [14C]-acetate into total lipid was higher in the flight-capable morph during this time. By contrast, activities of lipogenic enzymes and rates of lipid biosynthesis, in general, did not differ between morphs on a low nutrient diet during early adulthood. Differences in lipid biosynthesis account for previously documented differences in lipid reserves between morphs on some, but not all, diets. Results of the present and previous studies indicate that increased lipid biosynthesis in the flight capable morph on standard and high-carbohydrate diets constitutes an important adaptation for flight (production of lipid flight fuel). Lipid biosynthesis is negatively correlated with ovarian growth, and may be an important biochemical component of the trade-off between flight capability and ovarian growth in G. firmus. Morphs also differed in activities of three enzymes of lipid catabolism. However, the extent to which variation in activities of these enzymes between morphs results in variation in lipid catabolism is unclear. Finally, the flight-capable morph had a substantially higher activity of alanine aminotransferase in the fat body. Amino acids may be utilized for lipid biosynthesis or energy production to a greater degree in the dispersing morph compared with the oligately flightless morph. This study is the first to document differences in intermediary metabolism that underlie adaptations of morphs of a dispersal-polymorphic species for flight vs. egg production.

5.
J Insect Physiol ; 47(10): 1147-1160, 2001 Sep.
Article in English | MEDLINE | ID: mdl-12770193

ABSTRACT

The flight-capable morph of the wing-polymorphic cricket, Gryllus firmus, accumulated a substantially greater quantity of total lipid and triglyceride, compared with the obligately flightless morph, during the first five days of adulthood. Increased lipid accumulation in the flight-capable morph was genetically based, and was produced when ovarian growth is substantially reduced in that morph. Temporal changes in lipid levels suggest that the higher triglyceride reserves in the flight-capable morph fed a high-nutrient diet were produced by elevated lipid biosynthesis. By contrast, on a low-nutrient or high carbohydrate diet, increased lipid levels in the flight-capable morph appeared to result primarily from decreased lipid utilization. Increased biosynthesis or retention of triglyceride (the major flight fuel in Gryllus) by the flight-capable morph may significantly divert nutrients from egg production and hence may be an important physiological cause of its reduced ovarian growth. The obligately flightless morph allocated a greater proportion of total lipid to phospholipid than did the flight-capable morph. No functionally-significant differences in total lipid or triglyceride were produced between morphs during the last nymphal stadium. A second flightless morph, derived from the flight-capable morph by histolysis of flight muscles during adulthood, also had reduced amounts of total lipid and triglyceride but increased ovarian growth compared with the flight capable morph on the standard (high-nutrient) diet. Important qualitative and quantitative aspects of lipid metabolism differ genetically between the flight-capable and flightless morphs of G. firmus and likely contribute importantly to their respective adaptations for flight capability vs. reproduction. This is the first study to document genetically-based differences in energy reserves between morphs of a complex (phase, caste, flight) polymorphism in which morphs also differ genetically in key life history traits.

6.
Mol Phylogenet Evol ; 17(1): 48-57, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11020304

ABSTRACT

A well-supported molecular phylogeny for North American Gryllus species based on a combined data set of mitochondrial (mt) DNA is presented. A total of 26 individuals representing 13 populations of 11 species of the genus Gryllus and 4 individuals of two outgroup species, Teleogryllus oceanicus and Acheta domestica, were sampled in this study. The complete cytochrome b gene (1036 bp) and a 500-bp fragment of the 16S rRNA gene were sequenced for each individual. Since results from separate analyses of the cytochrome b and 16S data sets, as well as a previously published mtDNA restriction-site data set, were not conflicting, all data were combined for phylogenetic analyses. The clade of European Gryllus was clearly separated from the North American clade. The amount of sequence divergence between these clades was significantly greater than within the clades, suggesting a basal drift-vicariant event in the genus. This is the first phylogenetic analysis of North American Gryllus that includes western species. Four well-supported groups were identified but their relationships showed no clear east-west structure. Our phylogeny supports the recent reassignment of G. integer Scudder 1901 from Texas to G. texensis Cade and Otte 2000. The evolution of cricket song and life cycle is discussed using the new phylogenetic framework.


Subject(s)
DNA, Mitochondrial/genetics , Gryllidae/classification , Gryllidae/genetics , Animals , Cytochrome b Group/genetics , Evolution, Molecular , Genetic Variation , North America , Phylogeny , RNA, Ribosomal, 16S/genetics , Restriction Mapping
7.
J Insect Physiol ; 46(4): 585-596, 2000 Apr.
Article in English | MEDLINE | ID: mdl-12770222

ABSTRACT

Juvenile hormone titers and reproductive characteristics were measured in adult wing and flight-muscle morphs of the wing-polymorphic cricket, Gryllus firmus, during the first week of adulthood. This species has three morphs: one flight capable morph with fully-developed wings and fully-developed flight muscles [LW(F)], one flightless morph with fully-developed wings and histolyzed (non-functional) flight muscles [LW(H)], and another flightless morph with underdeveloped (short) wings and underdeveloped flight muscles (SW). Both flightless morphs [LW(H) and SW] had larger ovaries which contained a greater number of postvitellogenic eggs compared with the flight capable [LW(F)] morph. The juvenile hormone titer was significantly higher in SW compared with LW(F) females on days 3-7 of adulthood. On these days, the JH titer also was significantly higher in the other flightless morph, LW(H), compared with flight-capable [LW(F)] females as determined by one statistical test, but did not differ significantly by another test. The JH titer was positively correlated with ovarian mass or terminal oocyte length, but not with the number of post-vitellogenic eggs. This study is the first direct comparison of juvenile hormone titers in adult wing morphs of a wing-polymorphic insect. Results indicate that an elevated juvenile hormone titer may be at least partly responsible for one of the most distinctive features of wing-polymorphic species, the increased early fecundity of flightless females.

8.
J Insect Physiol ; 45(3): 275-285, 1999 Mar.
Article in English | MEDLINE | ID: mdl-12770375

ABSTRACT

Female Gryllus assimilis subjected to 4.5-7.7h continuous tethered flight had significantly lower amounts of total lipid, triglyceride and total soluble carbohydrate compared with unflown controls. A much greater amount of total lipid (6.3mg) was used during flight compared with carbohydrate (0.14mg). Flown individuals also had substantially reduced amounts of injected, radiolabeled [(14)C]-oleic acid. Activities of lipid, carbohydrate and amino acid catabolizing enzymes in flight muscles of G. assimilis and its wing-polymorphic congener, G. firmus, were very similar to activities in insects which primarily utilize lipid to power flight. By contrast, enzyme activities were very different from those in insects which primarily or exclusively use carbohydrate or proline as a flight fuel. These results strongly implicate lipid as the major flight fuel in Gryllus. Previous studies have shown that lipid levels are higher in flight-capable (long-winged) G. firmus that have small ovaries compared with flightless (short-winged) females that have large ovaries. Results of the present and previous studies collectively indicate that elevated lipid in long-winged G. firmus represents an energetic cost of flight capability which reduces (trade-offs with) reproduction in Gryllus. In G. firmus, mass-specific activities of nearly all enzymes were considerably reduced in underdeveloped, and to a lesser degree in histolyzed muscle, compared with fully-developed flight muscle. An important exception was alanine aminotransferase, whose activity was the highest in histolyzed muscle, and which may be involved in the catabolism of amino acids derived from muscle degradation. Despite the dramatic differences in enzyme activity, electrophoretic profiles of soluble flight-muscle proteins differed only subtly between fully-developed and underdeveloped or histolyzed flight muscles.

9.
Am Nat ; 152(1): 7-23, 1998 Jul.
Article in English | MEDLINE | ID: mdl-18811398

ABSTRACT

Adult Gryllus assimilis given an analog of juvenile hormone exhibited reduced flight muscles and enlarged ovaries similar to those found in naturally occurring flightless individuals of species that are polymorphic for dispersal capability. Control and hormone-treated (flightless) G. assimilis did not differ in the amount of food consumed or assimilated on any of three diets that differed in nutrient quantity. Thus, enhanced ovarian growth of flightless individuals resulted from increased allocation of internal nutrients to reproduction (i.e., a trade-off) rather than from increased acquisition of nutrients. Compared with flight-capable controls, flightless G. assimilis also had reduced whole-organism respiration, reduced respiration of flight muscles, and reduced lipid and triglyceride (flight fuel) reserves. These differences are remarkably similar to those between naturally occurring flightless and flight-capable morphs of other Gryllus species. Results collectively suggest that the increased allocation of nutrients to ovarian growth in flightless G. assimilis and other Gryllus species results from reduced energetic costs of flight muscle maintenance and/or the biosynthesis or acquisition of lipids. Reduction in these energetic costs appears to be an important driving force in the evolution of flightlessness in insects. Respiratory metabolism associated with flight capability utilizes an increasing proportion of the energy budget of crickets as the quantity of nutrients in the diet is decreased. This leads to a magnification of greater ovarian growth of flightless versus flight-capable individuals on nutrient-poor diets.

10.
Physiol Zool ; 70(5): 519-29, 1997.
Article in English | MEDLINE | ID: mdl-9279919

ABSTRACT

Flight muscles of the cricket Gryllus firmus are polymorphic, existing as pink or white phenotypes. White muscles are smaller in size, have reduced number and size of muscle fibers, and have reduced in vitro enzyme activities and respiration rates relative to pink muscles of newly molted, fully winged adults. G. firmus is also polymorphic for wing length. All newly molted long-winged adults exhibited the pink-muscle phenotype, while most newly molted short-winged adults exhibited the white-muscle phenotype, which resulted from arrested muscle growth. As long-winged adults aged, fully grown pink muscle was transformed into white muscle via histolysis. The substantially higher respiration rate of pink muscle likely contributes to the elevated whole-organism respiration rate of long-winged females, which has been documented previously and which is thought to divert nutrients from egg production. Histolyzed white flight muscle from long-winged crickets also exhibited significantly elevated respiration rate and enzyme activities compared with underdeveloped white muscle from short-winged adults, although these differences were not as great as those between pink and white muscles. Fecundity was much more elevated in females with white verus pink flight muscles than it was in females with short versus long wings. The fitness gain resulting from flightlessness has typically been estimated in previous studies by comparing enhanced egg production of short-winged and long-winged females, without considering the influence of flight-muscle variation. Our results suggest that the magnitude of this fitness gain has been substantially underestimated.


Subject(s)
Aging/physiology , Flight, Animal/physiology , Gryllidae/physiology , Muscles/physiology , Wings, Animal/physiology , Aging/metabolism , Analysis of Variance , Animals , Citrate (si)-Synthase/metabolism , Female , Gryllidae/anatomy & histology , Gryllidae/metabolism , Isocitrate Dehydrogenase/metabolism , Male , Muscles/enzymology , Muscles/metabolism , Organ Size/physiology , Ovary/anatomy & histology , Ovary/physiology , Oviposition/physiology , Oxygen Consumption/physiology , Phenotype , Reproduction/physiology , Sex Characteristics , Testis/anatomy & histology , Testis/physiology , Wings, Animal/anatomy & histology , Wings, Animal/metabolism
11.
Annu Rev Entomol ; 42: 207-30, 1997.
Article in English | MEDLINE | ID: mdl-15012313

ABSTRACT

Studies of dispersal polymorphism in insects have played a pivotal role in advancing our understanding of population dynamics, life history evolution, and the physiological basis of adaptation. Comparative data on wing-dimorphic insects provide the most definitive evidence to date that habitat persistence selects for reduced dispersal capability. The increased fecundity of flightless females documents that a fitness trade-off exists between flight capability and reproduction. However, only recently have studies of nutrient consumption and allocation provided unequivocal evidence that this fitness trade-off results from a trade-off of internal resources. Recent studies involving wing-dimorphic insects document that flight capability imposes reproductive penalties in males as well as females. Direct information on hormone titers and their regulation implicates juvenile hormone and ecdysone in the control of wing-morph determination. However, detailed information is available for only one species, and the physiological regulation of wing-morph production remains poorly understood. Establishing a link between the ecological factors that influence dispersal and the proximate physiological mechanisms regulating dispersal ability in the same taxon remains as a key challenge for future research.

12.
Biochem Genet ; 34(11-12): 421-35, 1996 Dec.
Article in English | MEDLINE | ID: mdl-9126671

ABSTRACT

In a previous study, activity of the insect endocrine regulator juvenile hormone esterase (JHE), in the cricket Gryllus assimilis, was subjected to bidirectional selection. This resulted in three pairs of high- and low-selected lines, each of which differed by 3.5-fold in JHE activity. In the present study, juvenile hormone esterases from these lines were characterized with respect to the Michaelis constant (K(m)), thermostability, and inhibition. None of three high-selected JHEs differed from its respective low-selected JHE in the Michaelis constant (K(m)) for juvenile hormone. Similarly, the high-selected JHEs did not differ from the low selected JHEs in thermostability or inhibition by either of two general esterase inhibitors (DFP, eserine) or a "JHE-specific" inhibitor (OTFP). Thus no evidence was obtained to suggest that the response to selection was due to allozymes or isozymes with altered kinetic or stability properties. Kinetic and stability properties were also very similar for the JHEs from the three high-selected or the three low-selected lines. Finally, none of the thermostability or inhibition profiles for any of the six JHEs exhibited sharp discontinuities, thus providing no evidence for the existence of multiple isozymes. The available evidence points to genetically variable regulators which affect the synthesis, degradation, or tissue distribution of JHE as being responsible for the divergence in JHE activity between the selected lines.


Subject(s)
Carboxylic Ester Hydrolases/metabolism , Gryllidae/enzymology , Juvenile Hormones/metabolism , Acetone/analogs & derivatives , Acetone/pharmacology , Animals , Carboxylic Ester Hydrolases/antagonists & inhibitors , Carboxylic Ester Hydrolases/chemistry , Enzyme Inhibitors/pharmacology , Enzyme Stability , Hemolymph/enzymology , Hot Temperature , Isoflurophate/pharmacology , Kinetics , Physostigmine/pharmacology
13.
Genetics ; 141(3): 1125-34, 1995 Nov.
Article in English | MEDLINE | ID: mdl-8582618

ABSTRACT

Hemolymph juvenile hormone esterase (JHE) activity on the third day of the last stadium in the cricket, Gryllus assimilis, exhibited a significant response to selection in each of six replicate lines. Mean realized heritability was 0.26 +/- 0.04. The response was due to changes in whole-organism enzyme activity as well as to changes in the proportion of enzyme allocated to the hemolymph compartment. In vivo juvenile hormone metabolism differed between some lines selected for high vs. low enzyme activity. Only minimal differences were observed between lines with respect to hemolymph protein concentration or whole-cricket activity of juvenile hormone epoxide hydrolase, the other major JH-degrading enzyme. Dramatic correlated responses to selection, equal in magnitude to the direct response, were observed for JHE activity on each of three other days of the last juvenile stadium. In contrast, no correlated responses in JHE activity were observed in adults. This indicates that JHE activities throughout the last stadium will evolve as a highly correlated unit independent of adult activities and the evolution of endocrine mechanisms regulating juvenile development can be decoupled from those controlling adult reproduction. This study represents the first quantitative-genetic analysis of naturally occurring endocrine variation in an insect species.


Subject(s)
Carboxylic Ester Hydrolases/blood , Gryllidae/enzymology , Age Factors , Animals , Biological Evolution , Breeding , Epoxide Hydrolases/blood , Female , Genetic Variation , Gryllidae/genetics , Gryllidae/growth & development , Hemolymph/enzymology , Male , Selection, Genetic
14.
Oecologia ; 80(2): 249-255, 1989 Aug.
Article in English | MEDLINE | ID: mdl-28313115

ABSTRACT

The genetic basis of wing morph determination and fertility differences between wing morphs were studied in the wing-dimorphic cricket, Gryllus rubens. Using pair corsses, a significant effect of genotype on morph determination was documented in F3 progeny of field-collected crickets. The effect of genotype was significantly stronger in females than in males. Results are consistent with an earlier study (Zera and Tiebel 1988) showing that wing development is more strongly buffered from environmental variation in females. Segregation patterns were consistent with a polygenic mode of inheritance and provided no evidence for the existence of genes of major effect, maternal effects, or sex linkage. Only a weak morph x sex association was observed. These results contrast those of Walker (1987) where sex-linked loci of major effect on morph determination were identified in crosses between long-wing and short-wing-selected strains of G. rubens. Short-winged female G. rubens began ovipositing earlier and oviposited significantly more eggs than long-winged females during the first 24 days after adult eclosion. The greater reproductive output of the short-winged morph was due entirely to greater oviposition during the first two weeks after adult eclosion. Preliminary results indicate that flight may further accentuate the reduced reproductive output of long-winged versus short-winged females. These data provide the foundation for investigating the endocrine basis of morph-associated fertility variation in G. rubens and its mechanistic relationship with morph determination.

15.
Mol Biol Evol ; 4(3): 266-85, 1987 May.
Article in English | MEDLINE | ID: mdl-3447011

ABSTRACT

Phosphoglucose isomerase (PGI) allozymes were isolated from the wing-polymorphic water strider, Limnoporus canaliculatus, and were characterized biochemically with respect to temperature-dependent kinetic and thermostability properties. At higher temperatures, the allozymes exhibited significant differences in Michaelis constant (Km) values for substrates of both the forward and reverse reaction directions. Results were consistent with expectations of adaptive kinetic differentiation based on the latitudinal variation of PGI allele frequencies. PGI genotypes also differed with regard to maximal velocity (Vmax)/Km ratios at higher temperatures. These differences were due primarily, if not exclusively, to allozyme-dependent variation in Km values. The allozymes also exhibited dramatic differences in thermostability. However, no thermostability differences were observed when the substrate analogue 6-phosphogluconate was present in the incubation medium. The data from this study, together with data from Mytilus edulis and Metridium senile on temperature-dependent kinetic variation among PGI allozymes, form a consistent picture of natural selection influencing the clinal variation of alleles at this locus in these three phylogenetically distant organisms. More definitive support of this hypothesis, however, must await additional studies on the physiological effects of the allozymic variation as well as direct measurements of fitness differences among the enzyme genotypes.


Subject(s)
Genetic Variation , Glucose-6-Phosphate Isomerase/genetics , Hemiptera/genetics , Isoenzymes/genetics , Alleles , Animals , Female , Gene Frequency , Genes , Glucose-6-Phosphate Isomerase/metabolism , Hemiptera/enzymology , Isoenzymes/metabolism , Kinetics , Male , Polymorphism, Genetic , Thermodynamics , Wings, Animal
16.
Biochem Genet ; 25(3-4): 205-23, 1987 Apr.
Article in English | MEDLINE | ID: mdl-3606558

ABSTRACT

Inhibition of phosphoglucose isomerase (PGI) allozymes from the wing-polymorphic waterstrider, Limnoporus canaliculatus, by three pentose-shunt metabolites was studied at several different temperatures. This was done to determine if the allozymes exhibited a differential ability to participate in lipid biosynthesis via differential partitioning of carbon flux through the pentose shunt versus glycolysis. 6-Phosphogluconate and erythrose-4-phosphate proved to be strong competitive inhibitors of PGI, while sedoheptulose-7-phosphate was a very weak inhibitor. The PGI allozymes from L. canaliculatus were differentially inhibited by 6-phosphogluconate at two of the three temperatures studied. However, this property does not appear to be an adaptive difference between the allozymes but, rather, a correlated effect resulting from variation in substrate binding. Estimates of reaction rates for the allozymes indicate that the differences in inhibition result in no detectable differences in reaction velocities. Thus, no evidence in support of the hypothesis that PGI allozymes from Limnoporus canaliculatus were adapted to function in different metabolic capacities via differential inhibition was obtained in this study. However, the importance of this characteristic in allozymic adaptation in natural populations remains an open question.


Subject(s)
Glucose-6-Phosphate Isomerase/antagonists & inhibitors , Hemiptera/metabolism , Pentose Phosphate Pathway , Adaptation, Physiological , Animals , Gluconates/pharmacology , Glucose-6-Phosphate Isomerase/genetics , Hemiptera/genetics , Kinetics , Polymorphism, Genetic , Sugar Phosphates/pharmacology
17.
Biochem Genet ; 19(7-8): 797-812, 1981 Aug.
Article in English | MEDLINE | ID: mdl-6794565

ABSTRACT

Variation at the alpha-glycerophosphate dehydrogenase (alpha-Gpdh; EC 1.1.1.8) locus was surveyed in 11 species of waterstriders (Gerridae: Hemiptera) and five other species of aquatic Hemiptera. Species of waterstriders exhibited considerable inter- and intraspecific variation in degree of winglessness. Average heterozygosity (0.401 +/- 0.090) and average number of observed electromorphs (5.36 +/- 0.96) for the 11 gerrid species were well above values reported for nearly all other insect species surveyed to date. Wing-monomorphic and wing-polymorphic species did not differ in average alpha Gpdh heterozygosity. Of the three wing-polymorphic species surveyed geographically, two species exhibited marked variation in wing-morph frequencies but homogeneous alpha-Gpdh allele frequencies. The third species exhibited geographically homogeneous alpha-Gpdh and wing-morph frequencies, but no significant association between alpha-Gpdh phenotype and wing morph was observed in any surveyed population. These results are consistent with hypotheses evoking either relaxed purifying selection at the alpha-Gpdh locus in species of Gerridae due to the apparent reduced importance of flight, or selective maintenance of common alpha-Gpdh electromorphs.


Subject(s)
Genetic Variation , Glycerolphosphate Dehydrogenase/genetics , Hemiptera/genetics , Alleles , Animals , Gene Frequency , Hemiptera/enzymology , Phenotype , Species Specificity
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