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Curr Protoc ; 4(3): e1014, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38506436

ABSTRACT

This article presents a practical guide to mass spectrometry-based data-independent acquisition and label-free quantification for proteomics analysis applied to cerebrospinal fluid, offering a robust and scalable approach to probing the proteomic composition of the central nervous system. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Cerebrospinal fluid sample collection and preparation for mass spectrometry analysis Basic Protocol 2: Mass spectrometry sample analysis with data-independent acquisition Support Protocol: Data-dependent mass spectrometry and spectral library construction Basic Protocol 3: Analysis of mass spectrometry data.


Subject(s)
Proteome , Proteomics , Humans , Proteomics/methods , Proteome/analysis , Mass Spectrometry/methods , Cerebrospinal Fluid Proteins/chemistry
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