Advances in bio/chemical approaches for sustainable recycling and recovery of rare earth elements from secondary resources.

Rare Earth Elements (REEs) are indispensable in the growing smart technologies, such as smart phones and electronic devices, renewable energy, new generation of hybrid cars, etc. These elements are naturally occurring in specific geological deposits (bastnäsite, monazite, and xenotime), primarily concentrated in the regions of China, Australia, and the USA. The extraction and processing of REEs and the mismanagement of secondary REE resources, such as industrial waste, end-of-life materials, and mining by-products, raise major environmental and health concerns. Recycling represents a convincing solution, avoiding the necessity to separate low-value or coexisting radioactive elements when REEs are recovered from raw ore. Despite these advantages, only 1 % of REEs are usually recycled. This review overreached strategies for recycling REEs from secondary resources, emphasizing their pivotal role. The predominant approach for recycling REEs involves hydrometallurgical processing by leaching REEs from their origins using acidic solutions and then separating them from dissolved impurities using techniques like liquid-liquid extraction, membrane separation, chromatography, adsorption, flotation, and electrochemical methods. However, these methods have notable disadvantages, particularly their over requirements for water, reagents, and energy. Biohydrometallurgy introduces an innovative alternative using microorganisms and their metabolites to extract REEs through bioleaching. Other investigations are carried out to recover REEs through biological strategies, including biosorption, affinity chromatography with biological ligands, bioflotation employing biological surfactants, and bioelectrochemical methods. However, biohydrometallurgical processes can also be relatively slow and less suitable for large-scale applications, often lacking specificity for targeted REEs recovery. Overcoming these challenges necessitates ongoing research and development efforts to advance recycling technologies.

Volatilization of mercury by immobilized bacteria (Klebsiella pneumoniae) in different support by using fluidized bed bioreactor.

Klebsiella pneumoniae, a mercury-resistant bacterial strain able to reduce ionic mercury to metallic mercury, was isolated from wastewater of Casablanca. This strain exhibits high minimal inhibition concentrations for heavy metals such as mercury 2400 microM, lead 8000 microM, silver 2400 microM, and cadmium 1000 microM. This bacterium was immobilized in alginate, polyacrylamide, vermiculite, and cooper beech and was used for removing mercury from a synthetic water polluted by mercury by using a fluidized bead bioreactor. Immobilized bacterial cells of Klebsiella pneumoniae could effectively volatilize mercury and detoxify mercury compounds. Moreover, the efficiency of mercury volatilization was much greater than with the native cells. The highest cleanup and volatilization rates were obtained when Klebsiella pneumoniae was entrapped in alginate beads, with a cleanup rate of 100% and a volatilization rate of 89%. Immobilized cells in alginate continuously volatilized mercury even after 10 days without loss of activity.

Waste water bacterial isolates resistant to heavy metals and antibiotics.

Sewage water of Casablanca, an industrial city in Morocco, was studied for microorganisms resistant to heavy metals. Isolates were purified and collected on agar slants to be screened for resistance to heavy metals, including mercury in vitro. The strains that showed high resistance to heavy metals were also studied for their resistance to antibiotics and aromatic hydrocarbons. Results indicated that the strains most resistant to all tested products belonged to Ps. fluorescens, Ps. aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus sp. These strains exhibit high minimal inhibitory concentrations for heavy metals such as cadmium (2 mm) or mercury (1.2 mm). Growth of Ps. fluorescens and Klebsiella pneumoniae in the presence of heavy metals was also determined, and the growth curves indicated that mercury, copper, and zinc present a slight inhibitory action, while cadmium and silver could have a potent inhibitory action on growth compared with the controls. These studies also investigated growth in media containing aromatic compounds as the sole source of carbon. The results demonstrate that these strains could be good candidates for remediation of some heavy metals and aromatic compounds in heavily polluted sites.