Edible Snail Production in Europe.

The human population is growing; food production is becoming insufficient, and the growing awareness of the negative impact of traditional animal husbandry on the environment means that the search for alternative methods of providing animal protein is continuously underway. The breeding of edible snails seems to be a promising option. The most popular species of edible snails in Europe include the brown garden snail Cornu aspersum (Müller, 1774) (previously divided into two subspecies: Cornu aspersum aspersum (Müller, 1774) and Cornu aspersum maxima (Taylor, 1883)), as well as the Roman Snail-Helix pomatia Linnaeus, 1758. These animals are highly productive, require relatively little space, are easy to breed and their maintenance does not require large financial outlays. This review focuses on the prospects of food snail farming in Europe. It discusses the living conditions, the nutritional value of the snails' meat, and the way of feeding the animals, paying particular attention to issues still not scientifically resolved, such as the need for micro and macro elements, as well as fat and carbohydrates.

Effect of radiant catalytic ionization on environmental conditions in rodent rooms and the haematological status of mice.

High stocking densities, closed animal houses, and elevated concentrations of bacteria, fungi, and the products of their activity, including ammonia and hydrogen sulphide, have adverse health effects. Active techniques used to reduce unfavourable environmental conditions, such as ventilation, sprinkling, bedding sorbents, and nutritional treatments, are not always sufficient to improve the animals' living environment. The current paper aims to evaluate the effect of radiant catalytic ionization (RCI) on airborne microorganisms, cage microbiological status, gaseous ammonia concentrations, and the haematological status of mice in animal houses. After one week of operation of an RCI system, the number of airborne bacteria and fungi in the experimental room decreased in comparison to the first day of the experiment (p < 0.05 and p < 0.05 respectively), as did the concentrations of ammonia (p < 0.01) and dust. At the same time, the basic health parameters of the mice, determined in the blood, were very similar between the control and experimental room. RCI seems to be an ideal solution to ensure high hygiene standards in animal rooms and houses with limited use of disinfectants or antibiotic treatment of sick animals. An additional, environmental benefit is the limited amount of nitrogen released.

Effect of extracts from eggs of Helix aspersa maxima and Helix aspersa aspersa snails on Caco-2 colon cancer cells.

Background: Colorectal cancer is the third most commonly diagnosed cancer. Natural compounds, administered together with conventional chemotherapeutic agent(s) and/or radiotherapy, may be a novel element in the combination therapy of this cancer. Considering the anticancer properties of compounds derived from different tissues of various snail species confirmed earlier, the purpose of the present research was to evaluate the effect of extracts from eggs of Helix aspera maxima and Helix aspersa aspersa snails, and fractions of extracts containing particles of different molecular weights on Caco-2 human epithelial colorectal adenocarcinoma cells. Methods: The extracts and fractions were analyzed for antioxidant activity, phenols and total carbohydrates using colorimetric methods. Lipid peroxidation products and glutathione in eggs were also examined using these methods. Crude protein and fat in eggs were determined. Molecular weights of egg proteins and glycoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Astaxanthin, selected vitamins and amino acids in eggs were measured using liquid chromatography methods, and minerals by emission spectroscopy, mass spectrometry or X-ray fluorescence. The action of extracts on the cell viability was determined by the MTT (methylthiazolyldiphenyl-tetrazolium bromide) test, based on the mitochondrial oxidative activity, after 24 and 72 h of treatment. The influence of fractions on the cell viability was assayed after 24 h. The effect of extracts on the percentage of live and dead cells was evaluated by the trypan blue assay, in which live cells exclude trypan blue, while dead cells take up this dye, after 12, 24, 48 and 72 h of treatment. Their influence on the integrity of cell membranes was determined based on the activity of LDH (lactate dehydrogenase), released from damaged cells, after 24 and 72 h of treatment. Then, the effect of extracts on the content of lipid peroxidation products in cells was examined using colorimetric method, after 24 h of treatment. Their influence on types of cell death was determined by flow cytometry, after this time. Results: The extracts and their fractions containing molecules <3 kDa decreased the cell viability, after 24 h of treatment. The extracts reduced the percentage of live cells (also after 48 h), increased the degree of cell membrane damage and the amount of lipid peroxidation products, induced apoptosis and reduced necrosis. Conclusions: Antioxidants, phenols, lipid peroxidation products, anticancer peptides, restriction of methionine, appropriate ratio of essential amino acids to non-essential amino acids, vitamin D3, Ca, Mg, S, Cu, Mn, Zn, Se and other bioactive compounds comprised in the extracts and their additive and synergistic effects may have influenced Caco-2 cells. Natural extracts or the chemical compounds contained in them might be used in the combination therapy of colorectal cancer, which requires further research.

Radiant catalytic ionization improves the microbiological status of rodent facilities without affecting the prooxidative status of mice.

The main microbial contaminants of rooms in which laboratory rodents are housed are bacteria and fungi. Restriction of microbial growth to below threshold levels requires the application of various sophisticated antimicrobial techniques that must be effective and safe for the animals. Some of the most commonly used techniques, including chemical disinfection, ventilation, filtration, sterilization and radiation, are not always sufficiently effective. The aim of the current study was to evaluate the efficacy of a modern technique (i.e. radiant catalytic ionization (RCI)) on the microbiological status of an animal care facility, and the health of the mice housed therein. The experiment, conducted over seven days, compared an experimental room with an RCI system permanently turned on with a negative control room. At the completion of the experiment, the number of bacteria in the RCI room air and on its walls was lower than that in the control room (p < 0.01 in both cases). Values of the basic prooxidative parameter, thiobarbituric acid reactive substances concentration, in tissues of mice from the RCI room were within allowed boundaries. Hence, application of an RCI system proved to be an ideal technique to ensure high hygienic standards in animal rooms without any adverse effects on the animals housed therein.

Impact of Ag Nanoparticles (AgNPs) and Multimicrobial Preparation (EM) on the Carcass, Mineral, and Fatty Acid Composition of Cornu aspersum aspersum Snails.

The hygienic practices on farms should reduce pathogenic microorganisms while simultaneously not harming the animals themselves; they must also not degrade the products' quality. We assessed the effect of covering feed tables with paint containing silver nanoparticles (AgNPs) and the periodic spraying of effective microorganisms (EM) on production indicators and basic chemical composition, mineral content and fatty acid profiles in the bodies of Cornu aspersum aspersum snails. The animals were divided into four groups: (1) control, (2) with feed tables covered with AgNPs paint, (3) with EM spray applied and (4) with both factors-AgNP paint and EM spray. The highest increase in Ag, Zn, Fe and Ca retention, and the remodelling of the fatty acid profile in the carcasses of snails was found to be in the group of animals in contact with the feed tables covered with AgNP paint. In the group of animals exposed to the action of EM, an increased retention of Fe, Cu, P, Mg and Zn was found.

Effect of Elaeagnus umbellata (Thunb.) fruit extract on H2O2-induced oxidative and inflammatory responses in normal fibroblast cells.

BACKGROUND: Elaeagnus umbellata is a plant commonly used in traditional Asian medicine for its many health benefits and strong antioxidative activity. Its therapeutic potential is believed to be connected to its effect on fibroblasts. This study aimed to investigate E. umbellata methanol-acetone extract's (EUE) defense against hydrogen peroxide (H2O2)-induced fibroblast damage. METHODS: Because the main biologically active compounds of E. umbellata are water-insoluble, we evaluated the effects of methanol-acetone fruit extracts using liquid chromatography (for ascorbic acid and beta-carotene) and spectrophotometry (for lycopene and total phenolics). The extract's antioxidative activity was measured using DPPH radical inhibition, and EUE's effect on human fibroblasts was also evaluated. We assessed the metabolic activity and apoptosis of HFFF-2 fibroblasts exposed to EUE and/or H2O2using the XTT test and flow cytometry, respectively. Superoxide dismutase activity and reactive oxygen species (ROS) production were evaluated using colorimetric and fluorometric assays, respectively. We measured pro-inflammatory cytokine (MIF, fractalkine, MCP-4, BLC, GCP-2, NAP-2, Eotaxin-2, and Eotaxin-3) expression in HFFF-2 cells using immunocytochemistry. RESULT: The extract increased HFFF-2 cell proliferation and reduced cell death caused by H2O2-induced stress. H2O2-treated fibroblasts had greater ROS production than cells treated with both H2O2 and EUE. Additionally, the group treated with H2O2 alone showed higher pro-inflammatory cytokine (MIF, MCP-4, NAP-2, Eotaxin-2, and Eotaxin-3) expression. CONCLUSION: EUE protected human fibroblasts from H2O2-induced oxidative stress and reduced the fibroblast-mediated inflammatory response triggered by ROS.

The Effect of Ag Nanoparticles and Multimicrobial Preparation as Factors Stabilizing the Microbiological Homeostasis of Feed Tables for Cornu aspersum (Müller) Snails on Snail Growth and Quality Parameters of Carcasses and Shells.

The aim of this research was to evaluate the effect of Ag nanoparticles (nano-Ag) used in the paint covering feed tables or a multimicrobial preparation applied to feed tables on the microbiological composition of the feed table environment, the growth and mortality of snails, and selected parameters for assessing the quality of carcasses and snail shells. The research was carried out in a farm of Cornu aspersum (Müller) snails. In the control (K) group, paint without nano-Ag was used. In two other groups (N-Ag and N-Ag + effective microorganisms (EM)), the feed tables were covered with the same paint as in the control group but with the addition of 100 mg/L of nano-Ag it (N-Ag group). Additionally, multimicrobial preparation (EM Bokashi®) at a concentration of 10% was spread on the tables in the N-Ag + EM group. In the last group (EM), the feed tables were covered with paint without nano-Ag, and only multimicrobial preparation was applied at a concentration of 10%. During the tests, the body weight of snails was measured three times, and swab samples were taken from the feed tables for the examination of microbiological composition. At the end of the experiment, the snails were killed, and the weight of the carcass and the size of the shell were measured. The content of Ag and the degree of lipid oxidation (thiobarbituric acid reactive substances (TBARS)) in the carcasses were analyzed, and the content of Ca and the crushing strength of the shells were determined. In the N-Ag and N-Ag + EM groups, a significant reduction in the total number of bacteria, fecal streptococci, and Escherichia coli was found, while there was also a reduction in mold and fungi in the N-Ag + EM and EM groups. In the K and EM groups, the mortality of animals was higher than in the nano-Ag groups. In subsequent weight checks, the highest body weight was found in the EM group and the lowest in the N-Ag and N-Ag + EM groups. In addition, the carcass weight and shell size in the N-Ag group was significantly lower compared to the K and EM groups. In the N-Ag and N-Ag + EM groups, a higher Ag content in the carcasses and a greater degree of lipid peroxidation were found. The Ca content of the shells was the highest in the N-Ag group, and the hardness of shells was the highest in the N-Ag and N-Ag + EM groups.