[The serological properties of a lysine producer developing in a batch culture]. / Serologicheskie svoistva produtsenta lizina, razvivaiushchegosia v periodicheskoi kul'ture.

Immune sera specific to bacterial cells at various development stages of periodic culture have been obtained with the purpose to study serological properties of lysin producer. Using the method of immunoenzyme analysis ELISA it is shown that there is certain dependence between the producer properties and the culture age. Different strains-producers used in production to-day are serologically close between each other. When using 49 strains-contaminants of lysin production (representatives of 10 genera) the absence of serological affinity between the producer and foreign microflora has been demonstrated. Preliminary data prove the prevalence of cells producers of thermostable antigens on the cell surface.

[Gram-negative bacteria contaminating the process of producing lysine]. / Gramotritsatel'nye bakterii, kontaminiruiushchie protsess proizvodstva lizina.

The authors have isolated Gram-negative bacteria of the Enterobacteriaceae family from the culture liquid of industrial fermenters with low yield of lysine. Most of them possessed the characters typical of Klebsiella pneumoniae and Escherichia coli, the rest were identified as the representatives of genera Proteus, Providencia, Enterobacter, Hafnia. Strains of Klebsiella pneumoniae, E.coli, Proteus rettgeri manifested lysine-decarboxylase activity. The capacity of some strains to destruct lysine synthesized by the target culture in the process of fermentation with formation of cadaverin was experimentally proved and confirmed under production conditions. Technological water is the source of distribution of gram-negative bacteria (first of all Klebsiella) in lysine production.

[The inhibition of the cellular immune response by Pseudomonas aeruginosa extracts]. / Ingibirovanie kletochnogo immunnogo otveta ékstraktami sinegnoinoi palochki.

Aquatic-phenol trichloroacetic and salt extracts of Pseudomonas aeruginosa have been studied for their effect on hypersensitivity of the delayed type to guinea pig antigen in mice. It is found that salt extracts of wild strains contain thermostable immunosuppressive factors which are of the lipid nature, have high molecular weight and can be inactivated by trichloroacetic acid and phenol. The salt extracts of museum strains as well as aquatic-phenol and trichloroacetic extracts obtained from wild and museum strains had no immunosuppressive activity.