ABSTRACT
OBJECTIVE: To screen the fractions from guava leaf to ascertain the effective anti-diarrhea fraction and to establish the method of quality control. METHODS: A series of samples from guava leaf were prepared through extraction and separation by solvents progressively, and their effect on the mice diarrhea models induced by senna decoction were tested individually; and the constituent correlated with the effect of the samples on the models was determined by HPLC. RESULTS: The effect of the fractions with moderate-polar glucosides from guava leaf was better than that of the other fractions; the effect of the fractions with the glucosides was correlated with the content of the quercetin glucosides in the fractions. CONCLUSION: The fractions with the moderate-polar glucosides is the effective anti-diarrhea fraction of guava leaf; determination of the quercetin glucosides can be used for quality control of guava leaf and its extracts.
Subject(s)
Antidiarrheals/pharmacology , Diarrhea/pathology , Drugs, Chinese Herbal/chemistry , Plant Leaves/chemistry , Psidium/chemistry , Quercetin/analysis , Animals , Antidiarrheals/chemistry , Antidiarrheals/isolation & purification , Chromatography, High Pressure Liquid , Diarrhea/chemically induced , Disease Models, Animal , Drugs, Chinese Herbal/isolation & purification , Drugs, Chinese Herbal/pharmacology , Female , Male , Mice , Plants, Medicinal/chemistry , Quality Control , Quercetin/chemistryABSTRACT
OBJECTIVE: To develop self-microemulsifying drug delivery system (SMEDDS) of Jiaotai Pill active components and its quality control method. METHODS: The solubility of berberine hydrochloride in different auxiliary-materials were investigated, and the self-microemulsion formula was optimized by studying the self-microemulsifying efficiency. The content of berberine hydrochloride and cinnamaldehyde in the preparation were determined by HPLC. RESULTS: The optimal self-microemulsion formula was composed of OP, propanediol, cinnamon oil, and total alkaloid from Rhizoma Coptidis with the ratio of 4:8:3:6. The average size of the microemulsion particle was 15.8 nm; the average content of berberine hydrochloride and cinnamaldehyde in the preparation was above 20.0% and 10.0%, respectively. CONCLUSION: The acquired microemulsion with small particle size is stable. The determination method of berberine hydrochloride and cinnamaldehyde in the preparation is accurate and reliable.
