ABSTRACT
This study introduces a meticulously constructed genome assembly at the chromosome level for the Rosaceae family species Prinsepia uniflora, a traditional Chinese medicinal herb. The final assembly encompasses 1272.71 megabases (Mb) distributed across 16 pseudochromosomes, boasting contig and super-scaffold N50 values of 2.77 and 79.32 Mb, respectively. Annotated within this genome is a substantial 875.99 Mb of repetitive sequences, with transposable elements occupying 777.28 Mb, constituting 61.07% of the entire genome. Our predictive efforts identified 49,261 protein-coding genes within the repeat-masked assembly, with 45,256 (91.87%) having functional annotations, 5127 (10.41%) demonstrating tandem duplication, and 2373 (4.82%) classified as transcription factor genes. Additionally, our investigation unveiled 3080 non-coding RNAs spanning 0.51 Mb of the genome sequences. According to our evolutionary study, P. uniflora underwent recent whole-genome duplication following its separation from Prunus salicina. The presented reference-level genome assembly and annotation for P. uniflora will significantly facilitate the in-depth exploration of genomic information pertaining to this species, offering substantial utility in comparative genomics and evolutionary analyses involving Rosaceae species.
Subject(s)
Rosaceae , Rosaceae/genetics , Molecular Sequence Annotation , Phylogeny , Genomics , DNA Transposable Elements/geneticsABSTRACT
Astragalus galactites is a medicinal plant. The total plastome length of A. galactites is 126,117 bp. It contains a large single-copy region of 69,805 bp, two inverted repeat regions of 20,638 bp, and a small single-copy region of 15,036 bp. The cp genome contains 110 complete genes, including 75 protein-coding genes (75 PCGs), 4 ribosomal RNA genes (4 rRNAs), and 30 tRNA genes (30 tRNAs). The overall GC content of cp DNA is 33.9%, the corresponding values of the LSC, SSC, and IR regions are 33.0%, 30.4%, and 43.3% respectively. The phylogenetic tree shows that A. galactites has the closest relationship with A. laxmannii.
