[Supercritical CO2 fluid extraction of Rosmarinus officinalis and capability of extracts eliminate OH radical].

OBJECTIVE: Supercritical CO2 fluid extraction process of antioxidation active components from Rosmarinus officinalis was studied. METHOD: A new extraction process of components extracted from R. officinalis by supercritical CO2 fluid extraction (SFE-CO2 ) was studied in detail. The capability of that the extract eliminate *OH radical was tested by electron paramagnetic resonance (EPR) technique and spin catch technique. RESULT AND CONCLUSION: With free radical clearance as index, by range and variance analysis, the optimum extraction process conditions were: keeping pressure at 30 MPa and temperature at 75 degrees C for 1 h, in the same time adding alcohol 0.30 mL x g(-1).