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1.
Plants (Basel) ; 13(10)2024 May 17.
Article in English | MEDLINE | ID: mdl-38794467

ABSTRACT

In the period 2022-2023, an analysis of fourteen phenotypic traits was conducted across 192 maize accessions in the Aral region of Xinjiang. The Shannon-Wiener diversity index was employed to quantify the phenotypic diversity among the accessions. Subsequently, a comprehensive evaluation of the index was performed utilizing correlation analysis, principal component analysis (PCA) and cluster analysis. The results highlighted significant findings: (1) A pronounced diversity was evident across the 192 maize accessions, accompanied by complex interrelationships among the traits. (2) The 14 phenotypic traits were transformed into 3 independent indicators through principal component analysis: spike factor, leaf width factor, and number of spikes per plant. (3) The 192 materials were divided into three groups using cluster analysis. The phenotypes in Group III exhibited the best performance, followed by those in Group I, and finally Group II. The selection of the three groups can vary depending on the breeding objectives. This study analysed the diversity of phenotypic traits in maize germplasm resources. Maize germplasm was categorised based on similar phenotypes. These findings provide theoretical insights for the study of maize accessions under analogous climatic conditions in Alar City, which lay the groundwork for the efficient utilization of existing germplasm as well as the development and selection of new varieties.

2.
PeerJ ; 12: e16838, 2024.
Article in English | MEDLINE | ID: mdl-38304185

ABSTRACT

Soil salinization is a widely recognized global environmental concern that has a significant impact on the sustainable development of agriculture at a global scale. Maize, a major crop that contributes to the global agricultural economy, is particularly vulnerable to the adverse effects of salt stress, which can hinder its growth and development from germination to the seedling stage. This study aimed to screen highly salt-tolerant maize varieties by using four NaCl concentrations of 0, 60, 120, and 180 mMol/L. Various agronomic traits and physiological and biochemical indices associated with salt tolerance were measured, and salt tolerance was evaluated using principal component analysis, membership function method, and GGE biplot analysis. A total of 41 local maize varieties were assessed based on their D values. The results show that stem thickness, germ length, radicle length, leaf area, germination rate, germination index, salt tolerance index, and seed vigor all decreased as salt concentration increased, while electrical conductivity and salt injury index increased with the concentration of saline solution. Under the stress of 120 mMol/L and 180 mMol/L NaCl, changes in antioxidant enzymes occurred, reflecting the physiological response mechanisms of maize under salt stress. Principal component analysis identified six major components including germination vigor, peroxidase (POD), plant height, embryo length, SPAD chlorophyll and proline (PRO) factors. After calculating the comprehensive index (D value) of each variety's performance in different environments using principal component analysis and the membership function method, a GGE biplot analysis was conducted to identify maize varieties with good salt tolerance stability: Qun Ce 888, You Qi 909, Ping An 1523, Xin Nong 008, Xinyu 66, and Hong Xin 990, as well as varieties with poor salt tolerance: Feng Tian 14, Xi Meng 668, Ji Xing 218, Gan Xin 2818, Hu Xin 712, and Heng Yu 369. Furthermore, it was determined that a 120 mMol/L NaCl concentration was suitable for screening maize varieties during germination and seedling stages. This study further confirmed the reliability of GGE biplot analysis in germplasm selection, expanded the genetic resources of salt-tolerant maize, and provided theoretical references and germplasm utilization for the introduction of maize in saline-alkali areas. These research findings contribute to a better understanding of maize salt tolerance and promote its cultivation in challenging environments.


Subject(s)
Salt Tolerance , Zea mays , Zea mays/genetics , Salt Tolerance/genetics , Reproducibility of Results , Sodium Chloride/pharmacology , Seedlings/genetics
3.
J Chromatogr A ; 1702: 464100, 2023 Aug 02.
Article in English | MEDLINE | ID: mdl-37263056

ABSTRACT

Herein, a chiral multishelled mesoporous carbon nanospheres (MCNs) with unique spiral multishelled hollow mesoporous chiral structure is synthesized; the MCNs can be used as stationary phases for high-resolution gas chromatography (GC) and have good separation capacity. The successful preparation of MCNs is verified by a variety of characterizations. In addition, the MCNs-coated capillary column shows excellent separation performance for n-alkanes, n-alcohols, aromatic compounds, and esters, and it has a faster analysis time than the HP-5 commercial capillary column. The chromatography separation performance for various isomers and racemates of the MCNs stationary phase was evaluated, and it showed good separation capability for amino acid derivatives. The MCNs-coated capillary column has been demonstrated to present good reproducibility and stability. In summary, all of the chromatography experiments in this work indicate that this new stationary phase of the MCNs has good application potential for GC capillary separation.


Subject(s)
Carbon , Nanospheres , Reproducibility of Results , Chromatography, Gas/methods , Esters
4.
J Mater Chem B ; 11(3): 631-639, 2023 01 18.
Article in English | MEDLINE | ID: mdl-36537727

ABSTRACT

Interferon-γ (IFN-γ) is one of the crucial inflammatory cytokines as an early indicator of multiple diseases. A fast, simple, sensitive and reliable IFN-γ detection method is valuable for early diagnosis and monitoring of treatment. In this work, we creatively developed an electrochemical aptasensor based on the topological material Bi2Se3 for sensitive IFN-γ quantification. The high-quality Bi2Se3 sheet was directly exfoliated from a single crystal, which immobilized the synthesized IFN-γ aptamer. Under optimal conditions, the electrochemical signal revealed a wide linear relation along with the logarithmic concentration of IFN-γ from 1.0 pg mL-1 to 100.0 ng mL-1, with the limit of detection as low as 0.5 pg mL-1. The topological material Bi2Se3 with Dirac surface states improved the electrochemical signal/noise ratio and thus the sensitivity of the sensors. Furthermore, this electrochemical aptasensor exhibited excellent specificity and stability, which could be attributed to the large-scale smooth surface of the Bi2Se3 sheet with few defects decreasing the non-specific absorption. The developed biosensor has the same good performance as the ELISA method for detecting the real serum samples. Our work demonstrates that the developed electrochemical aptasensors based on topological materials have great potential in the field of clinical determination.


Subject(s)
Aptamers, Nucleotide , Biosensing Techniques , Aptamers, Nucleotide/chemistry , Biosensing Techniques/methods , Interferon-gamma , Bismuth/chemistry , Selenium/chemistry
5.
Wei Sheng Yan Jiu ; 47(2): 246-276, 2018 Mar.
Article in Chinese | MEDLINE | ID: mdl-29903277

ABSTRACT

OBJECTIVE: To understand the status of community residents' satisfaction on food safety in Jiangxi Province. METHODS: Using stratified random sampling method, a total of 1216 residents of Jiangxi Province were selected. Questionnaire on food safety for residents was adopted. The investigation was conducted by means of door-to-door inquiry in July 2016. The main statistical method for satisfaction Likert score and rank test. RESULTS: The total satisfaction degree on food safety of 1216 residents in Jiangxi Province was 3. 00 ± 0. 59. The older, less educated, low-income people, rural residents 'satisfaction is higher( all P < 0. 05). 61. 5% of the residents thought the food "pesticide pollution"was serious, 54. 1% thought the food "illegal addition"was serious, 47. 7%thought the "microbial contamination " was serious, 42. 2% thought the "heavy metal pollution"was serious, 36. 2% thought "food safety information"was opaque, 34. 0%thought "food safety complaints channels"was not smooth, 33. 0% thought the crackdown on food safety behavior was low, 32. 0% was unsatisfied with "food safety knowledge propaganda". CONCLUSION: Jiangxi residents are not satisfied with food safety. Reducing pesticide pollution and heavy metal enrichment, preventing illegal addition, avoiding microbial pollution, strengthening the crackdownand food safety knowledge propaganda, openning complaints channelsand food safety information were urgent problems in food safety supervision in Jiangxi Province.


Subject(s)
Food Safety , Personal Satisfaction , China , Humans , Pesticide Residues , Rural Population , Surveys and Questionnaires
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