Androgen synthesis cell-specific CREBZF deficiency alters adrenal cortex steroid secretion and develops behavioral abnormalities in adult male mice.

The global challenge of male infertility is escalating, notably due to the decreased testosterone (T) synthesis in testicular Leydig cells under stress, underscoring the critical need for a more profound understanding of its regulatory mechanisms. CREBZF, a novel basic region-leucine zipper transcription factor, regulates testosterone synthesis in mouse Leydig cells in vitro; however, further validation through in vivo experiments is essential. Our study utilized Cyp17a1-Cre to knock out CREBZF in androgen-synthesis cells and explored the physiological roles of CREBZF in fertility, steroid hormone synthesis, and behaviors in adult male mice. Conditional knockout (cKO) CREBZF did not affect fertility and serum testosterone level in male mice. Primary Leydig cells isolated from CREBZF-cKO mice showed impaired testosterone secretion and decreased mRNA levels of Star, Cyp17a1, and Hsd3b1. Loss of CREBZF resulted in thickening of the adrenal cortex, especially X-zone, with elevated serum corticosterone and dehydroepiandrosterone levels and decreased serum dehydroepiandrosterone sulfate levels. Immunohistochemical staining revealed increased expression of StAR, Cyp11a1, and 17ß-Hsd3 in the adrenal cortex of CREBZF-cKO mice, while the expression of AR was significantly reduced. Along with the histological changes and abnormal steroid levels in the adrenal gland, CREBZF-cKO mice showed higher anxiety-like behavior and impaired memory in the elevated plus maze and Barnes maze, respectively. In summary, CREBZF is dispensable for fertility, and CREBZF deficiency in Leydig cells promotes adrenal function in adult male mice. These results shed light on the requirement of CREBZF for fertility, adrenal steroid synthesis, and stress response in adult male mice, and contribute to understanding the crosstalk between testes and adrenal glands.

Comparison of different drying technologies for kiwifruit pomace: Changes in physical characteristics, nutritional properties and antioxidant capacities.

The objective of this study was to evaluate the impacts of different drying technologies including microwave drying (MD), vacuum microwave drying (VMD), sun drying (SD), vacuum drying (VD), hot air drying (HAD), and vacuum freeze drying (VFD) on the physical characteristics, nutritional properties and antioxidant capacities of kiwifruit pomace in order to realize by-product utilization and improve energy efficiency. Results showed that both MD and VMD significantly reduced drying time by >94.6%, compared to traditional thermal drying which took 14-48 h. MD exhibited the highest content of soluble dietary fiber (9.5%) and the lowest energy consumption. Furthermore, VMD resulted in the highest content of vitamin C (198.78 mg/100 g) and reducing sugar (73.78%), and the antioxidant capacities ranked only second to VFD. Given the financial advantages and product quality, VMD was suggested to be advantageous technology in actual industrial production.

Use of buffer treatment to utilize local non-alkali tolerant bacteria in microbial induced calcium carbonate sedimentation in concrete crack repair.

Concrete often suffers cracks due to its low tensile strength. The repair process can vary ranging from surface coating, grouting, and strengthening. Microbial induced calcium carbonate sedimentation process (MICP) is a process of utilizing non-pathogenic bacteria to produce calcium carbonate through its urease activity in crack repair (filling). It is known as an alternative crack repair method that does not utilize Portland cement. In general, the bacteria used in MICP are alkali tolerant bacteria that have a higher chance of surviving the high alkalinity environment in concrete. However, in some regions, alkali tolerant bacteria are difficult to acquire and unavailable locally. This study introduced a technique to utilize non-alkali tolerant bacteria in MICP using buffer treatment. Instead of injecting bacteria directly onto the crack surface, the buffer solution was applied onto the crack surface prior to the bacteria injection. Results from the laboratory indicated a higher bacteria survival rate when the buffer treatment was applied to the medium. For the crack filling, with the buffer treatment, the crack was completely filled within 21-28 days. The microstructure results also showed that the crystal deposits from both laboratory and crack surface were similar in both physical appearance and phase composition.

Antioxidant Dipeptides Enhance Osmotic Stress Tolerance by Regulating the Yeast Cell Wall and Membrane.

This study aimed to investigate the role of the yeast cell wall and membrane in enhancing osmotic tolerance by antioxidant dipeptides (ADs) including Ala-His (AH), Thr-Tyr (TY), and Phe-Cys (FC). Results revealed that ADs could improve the integrity of the cell wall by restructuring polysaccharide structures. Specifically, FC significantly (p < 0.05) reduced the leakage of nucleic acid and protein by 2.86% and 5.36%, respectively, compared to the control. In addition, membrane lipid composition played a crucial role in enhancing yeast tolerance by ADs, including the increase of cell membrane integrity and the decrease of permeability by regulating the ratio of unsaturated fatty acids. The up-regulation of gene expression associated with the cell wall integrity pathway (RLM1, SLT2, MNN9, FKS1, and CHS3) and fatty acid biosynthesis (ACC1, HFA1, OLE1, ERG1, and FAA1) further confirmed the positive impact of ADs on yeast tolerance against osmotic stress.

Paralysis of the lower limbs caused by spontaneous spinal epidural hematoma: A case report.

INTRODUCTION AND IMPORTANCE: Spontaneous spinal epidural hematoma (SSEH) is a rare surgical emergency, often with acute onset, causing severe spinal cord injury. This report presents a case of SSEH treated by surgery to enhance understanding of this disease. CASE PRESENTATION: A 76-year-old male patient sought medical care for unexplained sudden chest and back pain and paralysis of both lower extremities. Magnetic resonance imaging (MRI) confirmed the occupation of thoracolumbar spinal canal. The blood clot was completely removed by emergency surgery, and then the neurological function recovered. CLINICAL DISCUSSION: Spontaneous spinal epidural hematoma is an uncommon disease, its acute onset, resulting in difficult to recover spinal cord injury, or even permanent paralysis. Therefore, early diagnosis and early operation are the key to the protection of neurological function. CONCLUSIONS: Surgical intervention is the first choice for treatment, especially in acute and subacute spontaneous spinal epidural hematoma. Once diagnosed, and there are no contraindications, it is recommended to remove and decompress the hematoma as soon as possible.

Oligodendrocytes Play a Critical Role in White Matter Damage of Vascular Dementia.

With the deepening of population aging, the treatment of cognitive impairment and dementia is facing increasing challenges. Vascular dementia (VaD) is a cognitive dysfunction caused by brain blood flow damage and one of the most common causes of dementia after Alzheimer's disease. White matter damage in patients with chronic ischemic dementia often occurs before cognitive impairment, and its pathological changes include leukoaraiosis, myelin destruction and oligodendrocyte death. The pathophysiology of vascular dementia is complex, involving a variety of neuronal and vascular lesions. The current proposed mechanisms include calcium overload, oxidative stress, nitrative stress and inflammatory damage, which can lead to hypoxia-ischemia and demyelination. Oligodendrocytes are the only myelinating cells in the central nervous system and closely associated with VaD. In this review article, we intend to further discuss the role of oligodendrocytes in white matter and myelin injury in VaD and the development of anti-myelin injury target drugs.

Hollow Bowl NiS2 @polyaniline Conductive Linker/Graphene Conductive Network: A Triple Composite for High-Performance Supercapacitor Applications.

The achievement of the outstanding theoretical capacitance of nickel sulfide (NiS2 ) is challenging due to its low conductivity, slow electrochemical kinetics, and poor structural stability. In this study, we utilize polyaniline (PANI) as a linker to anchor the NiS2 with a hollow bowl-like structure, uniformly dispersed at the surface of graphene oxide (GO)(NiS2 @15PG). The presence of PANI provides growth sites, resulting in a uniform and dense arrangement of NiS2 . This morphological modulation of NiS2 increases the contact area between the active material to electrolyte. Additionally, PANI effectively connects NiS2 with the conductive network of GO, which advances the electrical conductivity and ion diffusion properties. As a result, the Rct (charge transfer resistance) and Zw (Warburg impedance) of NiS2 @15PG decrease by 82.61 % and 66.76 % respectively. This unique structure confers NiS2 @15PG with high specific capacitance (536.13 C g-1 at 1 A g-1 ) and excellent multiplicative property of 60.93 % at 20 A g-1 . The assembled NiS2 @15PG//YP-50 supercapacitors (HSC) demonstrates an energy density (13.09 Wh kg-1 ) at a high-power density (16 kW kg-1 ). The capacity retention after 10,000 cycles at 5 A g-1 is 86.59 %, indicating its significant potential for practical applications.

Layered Structural PBAT Composite Foams for Efficient Electromagnetic Interference Shielding.

The utilization of eco-friendly, lightweight, high-efficiency and high-absorbing electromagnetic interference (EMI) shielding composites is imperative in light of the worldwide promotion of sustainable manufacturing. In this work, magnetic poly (butyleneadipate-co-terephthalate) (PBAT) microspheres were firstly synthesized via phase separation method, then PBAT composite foams with layered structure was constructed through the supercritical carbon dioxide foaming and scraping techniques. The merits of integrating ferroferric oxide-loaded multi-walled carbon nanotubes (Fe3O4@MWCNTs) nanoparticles, a microcellular framework, and a highly conductive silver layer have been judiciously orchestrated within this distinctive layered configuration. Microwaves are consumed throughout the process of "absorption-reflection-reabsorption" as much as possible, which greatly declines the secondary radiation pollution. The biodegradable PBAT composite foams achieved an EMI shielding effectiveness of up to 68 dB and an absorptivity of 77%, and authenticated favorable stabilization after the tape adhesion experiment.

2D/2D MgO/g-C3N4 S-scheme heterogeneous tight with Mg-N bonds for efficient photo-Fenton degradation: Enhancing both oxygen vacancy and charge migration.

Construction of S-scheme heterojunction is an efficient strategy to enhance photocatalytic efficiency. Besides the retained redox ability, the wide work function gap and intimate interface contact are essential for efficient degradation. Nontoxic magnesium oxide (MgO) with two dimensional (2D) structures and high work function is a potential material for S-scheme photocatalysts. Herein, MgO was used to in-situ grown on graphitic carbon nitride (g-C3N4) for constructing the strongly connected MgO/g-C3N4 S-scheme photocatalyst with tight Mg-N bonds. Meanwhile, the presence of Mg-N bonds induces the formation of oxygen vacancy in MgO, which enhances the Fenton-like degradation. Furthermore, the Mg-N bond promotes the charge migration between MgO and g-C3N4. Consisting of the enhanced Fenton-like process and photocatalysis, the MgO/g-C3N4 shows a higher photo-Fenton degradation activity (80.01%) for degradation of organic pollutants (Rhodamine B, 100 mg L-1) in water, than g-C3N4 (28.46%) and MgO (55.64%). Therefore, the interfacial chemical bonds in heterojunction photocatalysts provide an efficient strategy for further enhancing the photocatalysis of S-scheme photocatalysts.

Knee joint pain caused by synovial hemangioma: A case report.

INTRODUCTION AND IMPORTANCE: Knee joint synovial hemangioma is an uncommon condition primarily characterized by knee pain, often accompanied by other symptoms. This report presents a case of knee joint synovial hemangioma to enhance understanding of this condition. CASE PRESENTATION: An 18-year-old male patient sought medical attention due to unexplained knee pain. Physical examination revealed noticeable tenderness points in the knee joint. Magnetic resonance imaging (MRI) confirmed the presence of an internal knee bump. Arthroscopic surgery resulted in the complete resolution of symptoms. CLINICAL DISCUSSION: Knee joint synovial hemangioma is a rare ailment, and its relatively inconspicuous symptoms can lead to misdiagnosis, delayed treatment, or even overlooked cases. Therefore, it is crucial for healthcare professionals, especially young doctors, to be familiar with the distinctive attributes of this infrequent disorder in order to aid patients in regaining their normal lives. CONCLUSIONS: When encountering patients with unexplained knee pain, medical practitioners should consider the possibility of knee joint synovial hemangioma. Early identification and treatment are essential for prompt recovery and optimal outcomes for patients.

Mechanisms of Antioxidant Dipeptides Enhancing Ethanol-Oxidation Cross-Stress Tolerance in Lager Yeast: Roles of the Cell Wall and Membrane.

High concentrations of ethanol could cause intracellular oxidative stress in yeast, which can lead to ethanol-oxidation cross-stress. Antioxidant dipeptides are effective in maintaining cell viability and stress tolerance under ethanol-oxidation cross-stress. In this study, we sought to elucidate how antioxidant dipeptides affect the yeast cell wall and membrane defense systems to enhance stress tolerance. Results showed that antioxidant dipeptide supplementation reduced cell leakage of nucleic acids and proteins by changing cell wall components under ethanol-oxidation cross-stress. Antioxidant dipeptides positively modulated the cell wall integrity pathway and up-regulated the expression of key genes. Antioxidant dipeptides also improved the cell membrane integrity by increasing the proportion of unsaturated fatty acids and regulating the expression of key fatty acid synthesis genes. Moreover, the addition of antioxidant dipeptides significantly (p < 0.05) increased the content of ergosterol. Ala-His (AH) supplementation caused the highest content of ergosterol, with an increase of 23.68 ± 0.01% compared to the control, followed by Phe-Cys (FC) and Thr-Tyr (TY). These results revealed that the improvement of the cell wall and membrane functions of antioxidant dipeptides was responsible for enhancing the ethanol-oxidation cross-stress tolerance of yeast.

Bioactive dipeptides enhance the tolerance of lager yeast to ethanol-oxidation cross-stress by regulating the multilevel defense system.

Although high gravity brewing technology has been widely used for beer industries due to its economic benefits, yeast cells are subjected to multiple environmental stresses throughout the fermentation process. Eleven bioactive dipeptides (LH, HH, AY, LY, IY, AH, PW, TY, HL, VY, FC) were selected to evaluate their effects on cell proliferation, cell membrane defense system, antioxidant defense system and intracellular protective agents of lager yeast against ethanol-oxidation cross-stress. Results showed that the multiple stresses tolerance and fermentation performance of lager yeast were enhanced by bioactive dipeptides. Cell membrane integrity was improved by bioactive dipeptides through altering the structure of macromolecular compounds of the cell membrane. Intracellular reactive oxygen species (ROS) accumulation was significantly decreased by bioactive dipeptides, especially for FC, decreasing by 33.1%, compared with the control. The decrease of ROS was closely related to the increase of mitochondrial membrane potential, intracellular antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and glycerol level. In addition, bioactive dipeptides could regulate the expression of key genes (GPD1, OLE1, SOD2, PEX11, CTT1, HSP12) to enhance the multilevel defense systems under ethanol-oxidation cross-stress. Therefore, bioactive dipeptides should be potentially efficient and feasible bioactive ingredients to improve the multiple stresses tolerance of lager yeast during high gravity fermentation.

Scapulothoracic separation: A severe injury to the upper extremity.

INTRODUCTION AND IMPORTANCE: Scapulothoracic separation is an infrequent ailment marked by the detachment of the upper limb bones from the chest wall, resulting in a range of symptoms. In this report, we present a collection of instances of Scapulothoracic separation. CASE PRESENTATION: A female patient, aged 35, was referred to our emergency department from a primary healthcare center for treatment following a high-energy motor vehicle accident that occurred two days prior. Upon examination, no vascular damage was detected. Following the critical period, the patient underwent surgery to repair a clavicle fracture. Despite the passage of three months since the surgery, the patient continues to experience functional limitations in the affected limb. CLINICAL DISCUSSION: The incidence of Scapulothoracic separation.is uncommon and stems from forceful injuries, predominantly resulting from vehicular mishaps. In managing this condition, it is imperative to prioritize the individual's safety and prioritize targeted treatment thereafter. CONCLUSIONS: The presence or absence of vascular injury determines the need for emergency surgical treatment, while the presence or absence of neurological injury affects the recovery of limb function.

Oxygen vacancies-rich Cu-W18O49 nanorods supported on reduced graphene oxide for electrochemical reduction ofN2to NH3.

High-performance nitrogen fixation is severely limited by the efficiency and selectivity of a catalyst of electrochemical nitrogen reduction reaction (NRR) under ambient conditions. Here, the RGO/WOCu (reduced graphene oxide and Cu-doping W18O49) composite catalysts with abundant oxygen vacancies are prepared by the hydrothermal method. The obtained RGO/WOCu achieves an enhanced NRR performance (NH3 yield rate:11.4 µg h-1 mgcat-1, Faradaic efficiency: 4.4%) at -0.6 V (vs. RHE) in 0.1 mol L-1 Na2SO4 solution. Furthermore, the NRR performance of the RGO/WOCu still keeps at 95% after four cycles, demonstrating its excellent stability. The Cu+-doping increases the concentration of oxygen vacancies, which is conducive to the adsorption and activation of N2. Meanwhile, the introduction of RGO further improves the electrical conductivity and reaction kinetics of the RGO/WOCu due to the high specific surface area and conductivity. This work provides a simple and effective method for efficient electrochemical reduction ofN2.

Progress in the Foaming of Polymer-based Electromagnetic Interference Shielding Composites by Supercritical CO2.

As a critical action plan formulated for peaking carbon dioxide emissions, polymeric electromagnetic interference (EMI) shielding materials based on CO2 foaming technology have recently been attracting widespread attention in both research and industry, attributable to their efficient use of CO2 , high specific strength, corrosion resistance and low-cost characteristics. In the past decade, the emergence of novel design concepts and preparation techniques for CO2 foaming technology has led to the development of new high-performance EMI shielding materials in this field. This review summarizes the research progress made to date on the fabrication of EMI shielding composite foams by supercritical carbon dioxide (scCO2 ) foaming. We also explore the structure-activity relationships between the component/distribution and EMI shielding properties. Additionally, the application prospects and development challenges of new EMI shielding composite foams are described.

A clarification of concepts related to the digestion and absorption of carotenoids and a new standardized carotenoids bioavailability evaluation system.

The published literature has shown that there are various evaluation methods for the process of carotenoids digestion and absorption. However, it was found that many concepts are ambiguous in the evaluation process, and the selection of evaluation methods is inappropriate and inaccurate. These deficiencies trouble readers, hamper comparisons among different studies, and generate controversy in different literature. Therefore, it is imperative to establish a complete and standardized system for evaluating the digestion and absorption process of carotenoids. This review begins by clarifying confusing concepts during the process of carotenoids digestion and absorption, including the release rate, micellization rate, bioaccessibility, relative bioavailability and absolute bioavailability. Then this review discusses relevant factors affecting the key process of the digestion and absorption of carotenoids. Finally, a more specific and standardized system for evaluating carotenoids bioavailability was suggested based on four dimensions: intake, digestion, absorption and metabolic process. Clarifying concepts such as digestion and absorption and standardizing corresponding research methods will help to obtain reliable data and support interoperability and comparisons across studies.

A Novel Strategy for Alzheimer's Disease Based on the Regulatory Effect of Amyloid-ß on Gut Flora.

Alzheimer's disease (AD) is one of the most common neurodegenerative diseases worldwide. The accumulation of amyloid-ß (Aß) protein and plaque formation in the brain are two major causes of AD. Interestingly, growing evidence demonstrates that the gut flora can alleviate AD by affecting amyloid production and metabolism. However, the underlying mechanism remains largely unknown. This review will discuss the possible association between the gut flora and Aß in an attempt to provide novel therapeutic directions for AD treatment based on the regulatory effect of Aß on the gut flora.

A New Extension of the Topp-Leone-Family of Models with Applications to Real Data.

In this article, we proposed a new extension of the Topp-Leone family of distributions. Some important properties of the model are developed, such as quantile function, stochastic ordering, model series representation, moments, stress-strength reliability parameter, Renyi entropy, order statistics, and moment of residual life. A particular member called new extended Topp-Leone exponential (NETLE) is discussed. Maximum likelihood estimation (MLE), least-square estimation (LSE), and percentile estimation (PE) are used for the model parameter estimation. Simulation studies were conducted using NETLE to assess the MLE, LSE, and PE performance by examining their bias and mean square error (MSE), and the result was satisfactory. Finally, the applications of the NETLE to two real data sets are provided to illustrate the importance of the NETLG families in practice; the data sets consist of daily new deaths due to COVID-19 in California and New Jersey, USA. The new model outperformed many other existing Topp-Leone's and exponential related distributions based on the real data illustrations.

Use of Cement Mortar Incorporating Superabsorbent Polymer as a Passive Fire-Protective Layer.

Concrete structures, when exposed to fire or high temperatures for a certain time, could suffer partial damage or complete structural failure. Passive fire-protective coating materials are an alternative way to prevent or delay damage to concrete structures resulting from fire. Superabsorbent polymer (SP) is a synthetic material known for its ability to absorb and retain a large volume of water within itself. With this unique property, the SP exhibits great potential for use as a passive fire protection material. Although several studies have been carried out to investigate the effect of SP as a surface coating material for fire protection, very few have been investigated on the potential use of SP mixed with mortar as a passive fire-protective layer. The objective of this study is to introduce the use of SP in plastering mortar as a fire-protective layer for concrete subjected to temperatures up to 800 °C. This study is divided into two parts: (1) investigating the properties of cement mortar mixed with SP at 0.5% (CONC/SP-0.5) and 1.0% (CONC/SP-1.0) by weight of cement, and (2) investigating the potential use of SP mortar as a plastering layer for concrete subject to high temperatures. The experimental results showed that the density and compressive strength of SP mortar decreases with increasing SP dosages. From the heat exposure results, SP mortar exhibited lower strength loss due to the ability to mitigate moisture through its interconnected pore system. As for the use of SP mortar as a plastering layer, the results demonstrated the concrete specimen plastered with SP mortar had a lower temperature at the interface and core than that plastered with plain mortar. This led to a reduced strength loss of 20.5% for CONC/SP-0.5 and 17.2% for CONC/SP-1.0.

Laser-treated wood for high-efficiency solar thermal steam generation.

Solar-driven water vaporization is considered one of the most sustainable ways to solve water scarcity. The design of highly efficient solar absorber systems has received extensive attention. Here, we report a novel light absorption material for water evaporation using laser-treated wood. The obtained laser-treated wood possesses interconnected 3D porous networks formed by the random construction of carbon arrays and a hydrophilic surface due to the oxygen implantation by laser treatment. When under 1 sun solar-simulated light irradiation (1 kW m-2), the surface temperatures of dry and water-saturated wood reach 59.5 °C and 40.4 °C, respectively, indicating good heat localization. As a result, the laser-treated wood under 1 sun illumination shows high solar to vapor efficiencies of 93.1% and 92.6% for pure water and seawater, respectively, which are higher than that of most wood-based reported photo-thermal conversion materials. Therefore, the fabricated laser-treated wood may pave the way for harvesting solar energy to produce clean water at low cost.