Asprenols A-H, phenolic constituents from the stems of Ilex asprella.

Encouraged by the in vivo anti-inflammatory effect of aqueous extract of Ilex asprella stems, a further phytochemical investigation on I. asprella stems oriented by the in vitro NO production inhibition in RAW264.7 cells was conducted, which led to the isolation of eight new phenolic constituents, namely asprenols A-H (1-8), together with 12 known ones (9-20). The structures of the new compounds were established by extensive spectroscopic data analyses of HR-ESI-MS, IR, UV, and 1D and 2D NMR, and the absolute configurations were determined by comparison of experimental and calculated ECD analyses. All isolated were evaluated for the inhibition against NO production in RAW 264.7 cells, and several compounds showed moderate inhibitory effect.

Four new spirobenzylisoquinoline N-oxide alkaloids from the whole plant of Corydalis hendersonii.

Encouraged by the anti-myocardial ischemic effect of Corydalis hendersonii ethanol extract, a chemical reinvestigation of the whole plant of C. hendersonii was performed, which led to the isolation of four new spirobenzylisoquinoline N-oxide alkaloids, hendersines C-F (1-4), along with seven known isoquinoline alkaloids (5-11). The structures of the new isolates including absolute configurations were elucidated by the analysis of spectroscopic data and comparison of the experimental and calculated electronic circular dichroism (ECD) data. Compound 1 inhibited the NO production in lipopolysaccharide (LPS)-induced RAW 264.7 cells with an IC50 value of 70.3 µM, and increased the cell viabilities with 40.0 ±â€¯3.9% against the oxygen glucose deprivation injury in H9c2 cells at 40 µM.